Click on items in Services, Description, or Price for details and methods.
Prices listed are only for University of Washington Investigators, effective 10/19/09. Please contact Center Administrator for other pricing.
| Core |
Services |
Unit Description |
Price |
| Diabetes and Energy Balance |
Composition |
body mass - per mouse, minimum 5 mice |
$30 |
| tissue mass - per sample, minimum 5 samples |
$50 |
| Calorimetry |
first 36 hr measurement period - per mouse |
$240 |
| additional 24 hr measurement - per mouse |
$120 |
| data summary/interpretaion - per set (up to 8 mice/set) |
$200 |
| Meal Pattern Analysis |
24 hr measurement - per mouse |
under review |
Body Temperature
|
24 hr measurement - per mouse |
$30 |
| transponder implantation - per mouse |
$15 |
| Running Wheels Activity |
24 hr measurement - per mouse (Future Service) |
$40 |
| Diabetes Resource Usage |
per diem - per cage, per day |
$0.985 |
| experiment room - per day |
$80 |
| special diets |
variable |
Diabetes and Energy Balance-
Metabolic HUB |
Glucose Tolerance Tests |
per mouse (5 time points) |
$60 |
| Insulin Sensitivity Tests |
per mouse (5 time points) |
$60 |
| Treatment with Streptozotocin or Other Agents |
Drug Treatment |
$5 |
Animal Care
|
$15 |
| Necropsy |
sacrifice |
$2 |
| extract blood, serum, plasma samples |
$1 |
| excise tissue, up to 5 tissues |
$6 |
| excise tissue, > 5 tissues |
$10 |
| heart preparation |
$2 |
| Meta HUB Resource Usage |
control mice - each |
$60 |
| per diem - per cage, per day |
$0.985 |
| dissection room - per half day |
$35 |
| experiment room - per half day |
$60 |
| tech time - per hour |
$57 |
| special diets |
variable |
Diabetes and Energy Balance-
Analytical HUB |
General Chemistry |
|
| Glucose |
sample volumes of 80 uL |
$8 |
| Lipids |
|
| Lipid Extraction |
|
$4 |
| Free Fatty Acids |
sample volumes of 15 uL |
$10 |
| HDL (micro method) |
sample volumes of 10 uL |
$11 |
| Triglyceride TG (micro method) |
sample volumes of 10 uL |
$14 |
| Cholesterol TC (micro method) |
sample volumes of 10 uL |
$9 |
FPLC
(includes TC/TG & profile for fractions) |
|
$150 |
| Cytokines & Hormones |
|
| TNF Alpha |
Proteins are quantified using Luminex and Linco Kits. Costs are determined by the particular bead combinations and pricing provided by vendors. For example, adiponectin must be quantified apart from other proteins such as TNF, IL-6, and insulin. Approximate costs are below, but are subject to change. Please contact Center Administrator for exact pricing.
single-plex $15/sample
2-plex $25/sample
3-plex $27/sample
4-plex $32/sample
7-plex $38/sample
sample volumes of 10uL per sample done in duplicate required, so total 25uL per sample is optimal. |
| IL-6 |
| IL-4 |
| Leptin |
| Insulin |
| PAI-1 and others |
| Glucagon |
| Adiponectin |
| Urine Albumin |
per sample |
$12 |
| Urine Creatinine |
per sample |
$8 |
| BUN |
per sample |
$8 |
| Insulin (by Eliza) |
per sample |
$9 |
| Taqman PCR Quantification of Mouse Tissues |
Cyber Green per design |
$20 |
| Cyber Green per plate |
$30 |
| Primer Sets per design |
$120 |
| Primer Sets per plate |
$50 |
| Cardiovascular |
Echocardiography
(non-nvasive) |
basic measurements |
$70 |
| additional measurements |
$10 |
| data analysis and interpretation |
$60 |
Electrocardiography
(non-invasive) |
per animal |
under review |
| Invasive Hemodynamics |
surgical model w/basic measurements
(echocardiography required) |
$140 |
| additional measurements |
$10 |
| data analysis and interpretation |
$60 |
| Telemetry |
invasive |
$45 |
| Blood Pressure |
non-invasive |
under review |
| Carotid Stenosis |
surgical model w/arterial response to injury |
under review |
| arterial response to injury only |
under review |
| Myocardial Infarction Models |
permanent ligation |
$130 |
| ischemia reperfusion |
$200 |
| Hindlimb Ischemia |
surgical model w/blood flow measurements |
$210 |
| blood flow measurements only |
$140 |
| Other Surgical Models |
open thoracotomy |
$90 |
| vein catheter insertion |
under review |
| Bone Marrow Transplantation |
preparation of mice and bone marrow |
$61 |
| chimera determinations |
$29 |
| Drug Treatment |
injection |
$5 |
| post-op animal care |
$15 |
| Morphometry |
cardiac morphology |
variable |
| vascular morphology |
variable |
| Ultrasound Imaging |
aortic |
under review |
| renal artery/vein |
under review |
| Necropsy |
sacrifice |
$2 |
| extract blood, serum, plasma samples |
$1 |
| excise tissue, up to 5 tissues |
$6 |
| excise tissue, > 5 tissues |
$10 |
| heart preparation |
$2 |
| Cardiovascular Resource Usage |
control animal - per animal |
$60 |
| handling fee, technical support - per hour |
$55 |
| surgery procedures - per hour |
$60 |
| experiment room, low level - per half day |
$35 |
| experiment room, high level - per half day |
$60 |
| per diem - per cage, per day |
$0.985 |
| special diet |
variable |
Cardiovascular-
Vasoreactivity HUB |
Myography |
basic protocol - per ring (minimum of 6 rings/day) |
$60 |
| additional response curves - per ring (basic required) |
$10 |
Nephrology, Macrovascular, and Microvascular
*Morphology* |
Necropsy |
sacrifice |
$2 |
| extract blood, serum, plasma samples |
$1 |
| excise tissue, up to 5 tissues |
$6 |
| excise tissue, > 5 tissues |
$10 |
| heart preparation |
$2 |
| Tissue Processing |
trim & cassette - each |
$3 |
| process & embed tissue (paraffin) - per block |
$9 |
| Tissue Sectioning |
paraffin block sectioning, 1st slide - per slide |
$7 |
| frozen block sectioning, 1st slide - per slide |
$9 |
| additional unstained slides - per slide |
$3 |
| decalcification - each |
$5 |
| serial sections/aortic root (extra tech time) - per min |
$0.87 |
| Histology Stains |
H & E - per slide |
$5 |
| PAS - per slide |
$7 |
| Picrosirius Red - per slide |
$7 |
| Masson's Trichrome - per slide |
$7 |
| von Kossa - per slide |
$7 |
| Silver Methenamine - per slide |
$21 |
| Movats pentachrome - per slide |
$21 |
| Oil Red O - per slide |
$5 |
| Please inquire for other stains. |
variable |
| Immunochemistry |
IHC/FITC staining - per slide |
$19 |
batch staining (up to 48 slides) - per batch
*price is $218 plus price of primary antibody |
$218*
|
| new antibody work up - per antibody |
$218 |
| TUNEL (Apoptag plus) - per slide |
$32 |
| Electron Microscopy |
process & embed - per block |
$21 |
| thick section - per block |
$38 |
| thin section - per block |
$128 |
| scope time - per 30 mins |
$109 |
| In Situ Hybridization |
probe labelling, radioactive - per template |
$125 |
| plasmid linearization - per plasmid |
$51 |
| in situ hybridization (up to 48 slides) - per run |
$592 |
| Morphometric Analysis |
atherosclerosis, aortic root w/Movats - per animal |
$110 |
| atherosclerosis, aortic root w/Oil Red O - per animal |
$150 |
| atherosclerosis, aortic root w/Movats & ORO - per animal |
$110 |
| atherosclerosis, whole aorta, en face - per animal |
$110 |
| atherosclerosis, inominates - per animal |
$110 |
| kidney, glomerular size & percent matrix - per animal |
$110 |
| kidney, interstitial fibrosis - per animal |
$75 |
| other analysis |
variable |
| Nephrology Resource Usage |
paper slide tray (20 slides) - each |
$6 |
| slide storage box (100 slides) - each |
$15 |
| tech time - per hour |
$57 |
| RUSH fee |
double regular charge |
| shipping to your institution |
variable |
**Prices include blocking reagents, antibody retrieval/enzyme treatments, new slides, and cover slips.
Prices subject to change depending on antibody/reagent costs.** |
