Peg3 Is A Mediator Between p53 and Bax In DNA Damage-Induced Neuronal
Death
Johnson M.D., Wu X., Aithmitti N. and Morrison R.S.
Peg3 Is A Mediator Between p53 and Bax In DNA Damage-Induced Neuronal Death.
In Press, J. Biol. Chem., 2002.
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Peg3/Pw1 protein is increased in postnatal cortical
neurons by DNA damage. Peg3/Pw1 is a putative transcription factor
that was originally identified as being involved in the development of
the myogenic and neuronal lineages. Peg3/Pw1 was recently identified as
a gene product that is specifically expressed in fibroblasts undergoing
p53-mediated apoptosis when compared to cells undergoing p53-mediated
growth arrest. Therefore, we chose to examine its role in p53-mediated
neuronal cell death occurring after DNA damage. DNA damage induced a significant
increase in Peg3/Pw1 immunoreactivity (B2), in contrast to glutamate exposure
which had no effect on Peg3/Pw1 immunoreactivity (C2). The induction of
Peg3/Pw1 protein was confirmed by Western blot analysis. Additional studies
from our laboratory demonstrated that a dominant negative form of the
Peg3/Pw1 protein (inhibitory form of the protein) containing a truncated
C-terminus inhibits both Bax translocation and neuronal death occurring
after DNA damage.
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