Bax Involvement in p53-mediated Neuronal Cell Death
Xiang, H., Kinoshita, Y., Knudson, C. M., Korsmeyer,
S.J., Schwartzkroin, PA. and Morrison, R.S. Bax involvement in p53-mediated
neuronal cell death. J. Neurosci., 18:1363-1373, 1998.
Injury-induced alterations in neuronal morphology
and viability are dependent on the presence of Bax. Glutamate and
camptothecin induced a significant degree of cell death in neurons containing
the Bax gene (Wild-type) as demonstrated by their uptake of ethidium homodimer
(red fluorescence). In contrast, the majority of Bax-deficient cell bodies
that remained after glutamate and camptothecin treatment were healthy
as measured by the presence of calcein fluorescence (green) and the paucity
of ethidium homodimer staining (red fluorescence). These results demonstrate
that Bax is required for neuronal cell death in response to several forms
of cytotoxic injury.
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