NIDA Center

Molecular Basis of Drug Addiction
Pharmacology 534 - Autumn 2014

C Chavkin, N Stella, J Neumaier, P Phillips, L Zweifel, S Ferguson - Instructors

The class format starts with an introduction by the instructor followed by a discussion of the papers listed. Students are expected to have read the papers beforehand, and come to class prepared to discuss the methods, experimental design, and principal conclusion of the assigned papers. The course is designed to enhance the critical reading skills of the students and to familiarize the students with the strengths and limits of the key techniques in this research area.

3:30-5:30 PM
Charles Chavkin & Susan Ferguson Liu Z, Zhou J, Li Y, Hu F, Lu Y, Ma M, Feng Q, Zhang JE, Wang D, Zeng J, Bao J, Kim JY, Chen ZF, El Mestikawy S, Luo M. Dorsal raphe neurons signal reward through 5-HT and glutamate. Neuron. 81:1360-74.
Deroche-Gamonet V, Piazza PV. (2014) Psychobiology of cocaine addiction: Contribution of a multi-symptomatic animal model of loss of control. Neuropharmacology. 76 Pt B:437-49. (review)
Hyman SE, Malenka RC, Nestler EJ. (2006) Neural mechanisms of addiction: the role of reward-related learning and memory. Annu Rev Neurosci. 29:565-98. (review)
3:30-5:30 PM
Cannabinoids and addictive drugs: neuronal populations & molecular details (Stella with Erik Carlson, discussant) Tart CT. Marijuana intoxication common experiences. Nature. 226(5247):701-4.
Häring M, Kaiser N, Monory K, Lutz B. (2011) Circuit specific functions of cannabinoid CB1 receptor in the balance of investigatory drive and exploration. PLoS One. 6(11):e26617.
Ren Y, Whittard J, Higuera-Matas A, Morris CV, Hurd YL. (2009) Cannabidiol, a nonpsychotropic component of cannabis, inhibits cue-induced heroin seeking and normalizes discrete mesolimbic neuronal disturbances. J Neurosci. 29(47):14764-9.
3:30-5:30 PM
Genetics of nicotine addiction (Charles Chavkin with Zweifel, discussant) Bierut LJ. (2011) Genetic vulnerability and susceptibility to substance dependence. Neuron. 69(4):618-27. (review)
A. Kuryatov, W. Berrettini, J. Lindstrom (2011) Acetylcholine receptor (AChR) alpha5 subunit variant associated with risk for nicotine dependence and lung cancer reduces (alpha4beta2)alpha5 AChR function. Mol. Pharmacol., 79 : 119–125.
Smith JS, Schindler AG, Martinelli E, Gustin RM, Bruchas MR, Chavkin C. (2012) Stress-induced activation of the dynorphin/k-opioid receptor system in the amygdala potentiates nicotine conditioned place preference. J Neurosci. 32(4):1488-95.
3:30-5:30 PM
Plasticity associated with withdrawal and craving" ‘Stress potentiation of addiction risk (Neumaier with Sunila Nair, discussant) Krasnova IN, Marchant NJ, Ladenheim B, McCoy MT, Panlilio LV, Bossert JM, Shaham Y, Cadet JL. (2014) Incubation of methamphetamine and palatable food craving after punishment-induced abstinence. Neuropsychopharmacology. 39(8):2008-16.
Lee BR, Ma YY, Huang YH, Wang X, Otaka M, Ishikawa M, Neumann PA, Graziane NM, Brown TE, Suska A, Guo C, Lobo MK, Sesack SR, Wolf ME, Nestler EJ, Shaham Y, Schlüter OM, Dong Y. (2013) Maturation of silent synapses in amygdala-accumbens projection contributes to incubation of cocaine craving. Nat Neurosci. 16(11):1644-51.
Dong Y, Nestler EJ. (2014) The neural rejuvenation hypothesis of cocaine addiction. Trends Pharmacol Sci. 35(8):374-83. (review)
3:30-5:30 PM
Drug-induced plasticity in dopamine neurons (Zweifel with Nephi Stella, discussant ) Liu QS, Pu L, Poo MM. (2005) Repeated cocaine exposure in vivo facilitates LTP induction in midbrain dopamine neurons. Nature. 437:1027-31.
Polter AM, Kauer JA. (2014) Stress and VTA synapses: implications for addiction and depression. Eur J Neurosci. 39(7):1179-88. (review)
Bocklisch C, Pascoli V, Wong JC, House DR, Yvon C, de Roo M, Tan KR, Lüscher C. (2013) Cocaine disinhibits dopamine neurons by potentiation of GABA transmission in the ventral tegmental area. Science. 341(6153):1521-5.
3:30-5:30 PM
Striatal circuitry and drug-seeking (Ferguson with Paul Phillips, discussant) MacAskill AF, Cassel JM and Carter AG (2014) Cocaine exposure reorganizes cell type- and input-specific connectivity in the nucleus accumbens. Nature Neuroscience 17(9):1198-207.
Britt JP, Benaliouad F, McDevitt RA, Stuber GD, Wise RA, Bonci A (2012) Synaptic and behavioral profile of multiple glutamatergic inputs to the nucleus accumbens. Neuron 76(4):790-803.
Stuber GD, Mason AO (2013) Integrating optogenetic and pharmacological approaches to study neural circuit function: current applications and future directions. Pharmacol Rev 65(1)156-70. (review)
11/6/14 Neurochemical changes associated with escalated and compulsive drug use (Phillips with Susan Ferguson, discussant) Pelloux Y, Dilleen R, Economidou D, Theobald D, Everitt BJ (2012) Reduced forebrain serotonin transmission is causally involved in the development of compulsive cocaine seeking in rats. Neuropsychopharmacology 37(11):2505-14.
Chen BT, Yau HJ, Hatch C, Kusumoto-Yoshida I, Cho SL, Hopf FW, Bonci A (2013) Rescuing cocaine-induced prefrontal cortex hypoactivity prevents compulsive cocaine seeking. Nature. 496(7445):359-62.
Everitt BJ (2014) Neural and psychological mechanisms underlying compulsive drug seeking habits and drug memories--indications for novel treatments of addiction. Eur J Neurosci 40(1):2163-82. (review)
11/13/14 Serotonin receptors & Hallucinogens (Neumaier with Elyse Dankowski, discussant ) Karaki S, Becamel C, Murat S, Mannoury la Cour C, Millan MJ, Prézeau L, Bockaert J, Marin P, Vandermoere F. (2014)Quantitative phosphoproteomics unravels biased phosphorylation of serotonin 2A receptor at Ser280 by hallucinogenic versus nonhallucinogenic agonists. Mol Cell Proteomics. 13(5):1273-85.
González-Maeso J, Weisstaub NV, Zhou M, Chan P, Ivic L, Ang R, Lira A, Bradley-Moore M, Ge Y, Zhou Q, Sealfon SC, Gingrich JA. (2007) Hallucinogens recruit specific cortical 5-HT(2A) receptor-mediated signaling pathways to affect behavior. Neuron. 53(3):439-52.
Benningfield MM, Cowan RL. (2013) Brain serotonin function in MDMA (ecstasy) users: evidence for persisting neurotoxicity. Neuropsychopharmacology. 38(1):253-5. (review)
11/20/14 Ethanol Addiction (Clark with Abbie Schindler, discussant ) Cuzon Carlson VC1, Seabold GK, Helms CM, Garg N, Odagiri M, Rau AR, Daunais J, Alvarez VA, Lovinger DM, Grant KA. (2011) Synaptic and morphological neuroadaptations in the putamen associated with long-term, relapsing alcohol drinking in primates. Neuropsychopharmacology. 2011 Nov;36(12):2513-28.
Corbit LH, Nie H, Janak PH. (2012) Habitual alcohol seeking: time course and the contribution of subregions of the dorsal striatum. Biol Psychiatry. 72(5):389-95.
Koob GF. (2004) A role for GABA mechanisms in the motivational effects of alcohol. Biochem Pharmacol. (2004) 68(8):1515-25. (review)
3:30-5:30 PM
General Discussion Marrs W, Stella N. (2009) Satel S, Lilienfeld SO. (2014) Addiction and the brain-disease fallacy. Front Psychiatry. 4:141.