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Molecular Basis of Drug Addiction
Pharmacology 534 - Autumn 2004

C Chavkin, K Mackie, N Stella, J Neumaier, and P Phillips - Instructors

The class format starts with an introduction by the instructor followed by a discussion of the papers listed. Students are expected to have read the papers beforehand, and come to class prepared to discuss the methods, experimental design, and principal conclusion of the assigned papers. The course is designed to enhance the critical reading skills of the students and to familiarize the students with the strengths and limits of the key techniques in this research area.

9/30/04
2:00-4:00 PM
Introduction - Reward pathways (CC) Kalivas PW. Glutamate systems in cocaine addiction. Curr Opin Pharmacol. 4(1):23-9 (2004).
 
Nestler EJ. Historical review: Molecular and cellular mechanisms of opiate and cocaine addiction. Trends Pharmacol Sci. 25(4):210-8 (2004).
 
Deroche-Gamonet V, Belin D, Piazza PV. Evidence for addiction-like behavior in the rat. Science 305(5686):1014-7 (2004).
10/7/04
4:00-6:00 PM
Nicotine Addiction (CC) Batra V, Patkar AA, Berrettini WH, Weinstein SP, Leone FT. The genetic determinants of smoking. Chest. 123(5):1730-9 (2003).
 
Stolerman IP, Chamberlain S, Bizarro L, Fernandes C, Schalkwyk L. The role of nicotinic receptor alpha 7 subunits in nicotine discrimination. Neuropharmacology. 46(3):363-71 (2004).
 
Champtiaux N, Gotti C, Cordero-Erausquin M, David DJ, Przybylski C, Lena C, Clementi F, Moretti M, Rossi FM, Le Novere N, McIntosh JM, Gardier AM, Changeux JP. Subunit composition of functional nicotinic receptors in dopaminergic neurons investigated with knock-out mice. J Neurosci. 23(21):7820-9 (2003).
10/14/04
4:00-6:00 PM
Transporters and Drug Self-administration (JN) Mateo Y, Budygin EA, John CE, Jones SR. Role of serotonin in cocaine effects in mice with reduced dopamine transporter function. Proc Natl Acad Sci U S A. 101:372-7 (2004).
 
Mead AN, Rocha BA, Donovan DM, Katz JL. Intravenous cocaine induced-activity and behavioural sensitization in norepinephrine-, but not dopamine-transporter knockout mice. Eur J Neurosci. 16:514-20 (2002).
10/21/04
4:00-6:00 PM
Cocaine effects on reward circuitry (PP) Borgland SL, Malenka RC, Bonci A. Acute and chronic cocaine-induced potentiation of synaptic strength in the ventral tegmental area: electrophysiological and behavioral correlates in individual rats. J Neurosci 24:7482-7490 (2004).
 
Kolb B, Gorny G, Li Y, Samaha AN, Robinson TE. Amphetamine or cocaine limits the ability of later experience to promote structural plasticity in the neocortex and nucleus accumbens. Proc Natl Acad Sci USA 100:10523-10528 (2003).
 
Phillips PEM, Stuber GD, Heien MLAV, Wightman RM, Carelli RM. Subsecond dopamine release promotes cocaine seeking. Nature 422:614-618 (2003).
10/28/04
4:00-6:00 PM
Endocannabinoid inactivation and drug addiction (NS) Sipe JC, Chiang K, Gerber AL, Beutler E, Cravatt BF. A missense mutation in human fatty acid amide hydrolase associated with problem drug use. Proc Natl Acad Sci U S A.;99(12):8394-9 (2002).
 
Chiang KP, Gerber AL, Sipe JC, Cravatt BF. Reduced cellular expression and activity of the P129T mutant of human fatty acid amide hydrolase: evidence for a link between defects in the endocannabinoid system and problem drug use. Hum Mol Genet. 13(18):2113-9 (2004).
 
Marsicano G, Wotjak CT, Azad SC, Bisogno T, Rammes G, Cascio MG, Hermann H, Tang J, Hofmann C, Zieglgansberger W, Di Marzo V, Lutz B. The endogenous cannabinoid system controls extinction of aversive memories. Nature. 418(6897):530-4 (2002).
 
Piomelli D. The molecular logic of endocannabinoid signalling. Nat Rev Neurosci.;4(11):873-84 (2003).
11/4/04
4:00-6:00 PM
Endocannabinoids and short term synaptic plasticity. (KM) Wilson RI, Kunos G, Nicoll RA. Presynaptic specificity of endocannabinoid signaling in the hippocampus. Neuron 31(3):453-462 (2001).
 
Brager DH, Luther PW, Erdelyi F, Szabo G, Alger BE. Regulation of exocytosis from single visualized GABAergic boutons in hippocampal slices. J. Neurosci 23(33):10475-10486 (2003).
 
Kim J, Alger BE. Inhibition of cyclooxygenase-2 potentiates retrograde endocannabinoid effects in hippocampus. Nat. Neurosci. 7(7):697-698 (2004).
 
Carlson G, Wang Y, Alger BE. Endocannabinoids facilitate the induction of LTP in the hippocampus. Nat. Neurosci 5(8):723-724 (2002).
Note change in day
11/9/04
4:30-6:30 PM
Endocannabinoid production and memory (NS) Kelley BG, Thayer SA. Delta 9-tetrahydrocannabinol antagonizes endocannabinoid modulation of synaptic transmission between hippocampal neurons in culture. Neuropharmacology. 46(5):709-15 (2004).
 
Losonczy A, Biro AA, Nusser Z. Persistently active cannabinoid receptors mute a subpopulation of hippocampal interneurons. Proc Natl Acad Sci U S A. 101(5):1362-7 (2004).
 
Brenowitz SD, Regehr WG. Calcium dependence of retrograde inhibition by endocannabinoids at synapses onto Purkinje cells. J Neurosci. 23(15):6373-84 (2003).
11/18/04
4:00-6:00 PM
Endocannabinoids, cannabis, cocaine, and long term synaptic plasticiy (KM) Fourgeaud L, Mato S, Bouchet D, Hemar A, Worley PF, Manzoni OJ. A single in vivo exposure to cocaine abolishes endocannabinoid-mediated long-term depression in the nucleus accumbens. J Neurosci. 24(31):6939-45 (2004).
 
Mato S, Chevaleyre V, Robbe D, Pazos A, Castillo PE, Manzoni OJ. A single in-vivo exposure to delta 9THC blocks endocannabinoid-mediated synaptic plasticity. Nat Neurosci. 7(6):585-6 (2004).
 
Robbe D, Kopf M, Remaury A, Bockaert J, Manzoni OJ. Endogenous cannabinoids mediate long-term synaptic depression in the nucleus accumbens. Proc Natl Acad Sci U S A. 99(12):8384-8 (2002).
 
Chevaleyre V, Castillo PE. Endocannabinoid-mediated metaplasticity in the hippocampus. Neuron. 43(6):871-81 (2004).
Note change in day
11/23/04
4:30-6:30 PM
Models of Reinstatement and Relapse (JN) Ciccocioppo R, Economidou D, Fedeli A, Angeletti S, Weiss F, Heilig M, Massi M. Attenuation of ethanol self-administration and of conditioned reinstatement of alcohol-seeking behaviour by the antiopioid peptide nociceptin/orphanin FQ in alcohol-preferring rats. Psychopharmacology (Berl). 172(2):170-8 (2004).
 
Fuchs RA, Evans KA, Ledford CC, Parker MP, Case JM, Mehta RH, See RE. The Role of the Dorsomedial Prefrontal Cortex, Basolateral Amygdala, and Dorsal Hippocampus in Contextual Reinstatement of Cocaine Seeking in Rats. Neuropsychopharmacology. 2004 Oct 13; [Epub ahead of print]
 
Lu L, Grimm JW, Hope BT, Shaham Y. Incubation of cocaine craving after withdrawal: a review of preclinical data. Neuropharmacology. 47 Suppl 1:214-26 (2004).
12/2/04
4:00-6:00 PM
Neuroimaging (PP) McClure SM, York MK, Montague PR. The neural substrates of reward processing in humans: the modern role of FMRI. Neuroscientist. 10(3):260-268 (2004).
 
Febo M, Segarra AC, Tenney JR, Brevard ME, Duong TQ, Ferris CF. Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats. J Neurosci Methods 139(2):167-176 (2004).
 
Chen YC, Choi JK, Andersen SL, Rosen BR, Jenkins BG. Mapping dopamine D2/D3 receptor function using pharmacological magnetic resonance imaging. Psychopharmacology (2005) [Epub ahead of print].
 
Volkow ND, Wang GJ, Ma Y, Fowler JS, Zhu W, Maynard L, Telang F, Vaska P, Ding YS, Wong C, Swanson JM. Expectation enhances the regional brain metabolic and the reinforcing effects of stimulants in cocaine abusers. J Neurosci 23(36):11461-11468 (2003).
12/9/04
4:00-6:00 PM
Genetic Approaches: QTLs, Microarrays and Candidate Genes (CC) Kreek MJ, Nielsen DA, LaForge KS. Genes associated with addiction: alcoholism, opiate, and cocaine addiction. Neuromolecular Med. 5(1):85-108 (2004).
 
Ferraro TN, Golden GT, Smith GG, Martin JF, Schwebel CL, Doyle GA, Buono RJ, Berrettini WH. Confirmation of a Major QTL Influencing Oral Morphine Intake in C57 and DBA Mice Using Reciprocal Congenic Strains. Neuropsychopharmacology. (2004) [Epub ahead of print]
 
Tapper AR, McKinney SL, Nashmi R, Schwarz J, Deshpande P, Labarca C, Whiteaker P, Marks MJ, Collins AC, Lester HA. Nicotine activation of alpha4* receptors: sufficient for reward, tolerance, and sensitization. Science. 306(5698):1029-32 (2004).