HLADIK, MD, PhD, Florian
Research Assistant Professor
Affiliate Investigator, Vaccine and Infectious Disease Division
Fred Hutchinson Cancer Research Center (FHCRC)
• MD, PhD degrees
(University of Vienna, Austria, 1993)
• Residency in Dermatology and Sexually Transmitted Diseases
(University of Vienna, Austria, 1995)
• Fellowship in Infectious Diseases
(University of Washington, 1998)
• Fellowship in Dermatology and Sexually Transmitted Diseases
(University of Vienna, Austria, 2000)
• Dermatology and Sexually Transmitted Diseases
(European Union, 2000)
• Dermatology and Sexually Transmitted Diseases
(University of Vienna, Austria, 2003)
• Pathogenesis of mucosal HIV transmission
• Development and toxicity evaluation of topical anti-HIV microbicides
• Immunology of the reproductive tract
• Evaluation of mucosal immune responses to HIV vaccination
• The role of exosomes in semen for HIV infection in the genital mucosa
in women (UW Royalty Research Award & NIH R21 award)
• Standardization and comparative assessment of mucosal sampling
techniques for evaluating vaccine-induced immune responses
(HIV Vaccine Trials Network Genitourinary Working Group Award)
- International collaboration (Seattle, Chicago Nairobi, Cape Town)
• Optimization of T lymphocyte and antigen-presenting cell (APC)
recovery from frozen genital secretions and tissues in women (HIV
Vaccine Trials Network Genitourinary Working Group) - Collaboration
with UW Department of Mechanical Engineering
• Mucosal anti-HIV antibodies in exposed uninfected women participating
in microbicide trials (Microbicide Trials Network Ancillary Project, Bill
and Melinda Gates Foundation)
• Associate Director, Microbicide Trials Network Immunology Core
- Discovery of microbicide toxicity biomarkers using global gene
- Impact of sytemic and topical pre-exposure prophylaxis on the
immunological course of break-through HIV infection
- Monitoring of peripheral blood mononuclear cell processing, freezing
and storage procedures at participating MTN trial sites in Africa
• Co-investigator, HIV Vaccine Trials Network Laboratory Program
- Vaccine-related changes of the mucosal immune environment
• Co-investigator, Viral determinants of HIV-1 transmission in African
heterosexuals (NIH Program Project)
• Co-investigator, Nanoparticle microbicides for delivery of combination
antiretroviral drugs - Collaboration with UW Department of Bioengineering
(NIH R21/R33 award)
M. J. McElrath, L. Ballweber, A. Terker, A. Kreger, P. Sakchalathorn, B.Robinson, M. Fialkow, G. Lentz, F. Hladik. Ex vivo comparison ofmicrobicide efficacies for preventing HIV-1 genomic integration in intraepithelial vaginal cells. Antimicrobial Agents and Chemotherapy 2010, 54:763-72.
J. Zhu, F. Hladik, A. Woodward, A. Klock, T. Peng, C. Johnston, M. Remington, A. Magaret, D. M. Koelle, A. Wald, L. Corey. Persistence of HIV-1 receptor-positive cells after HSV-2 reactivation is a potential mechanism for increased HIV-1 acquisition. Nature Medicine 2009, 15:886-93.
B. Doehle, F. Hladik, J. McNevin, M. J. McElrath, M Gale. HIV antagonizes innate antiviral signaling pathways by targeting Interferon Regulatory Factor-3. Journal of Virology 2009, 83:10395-405.
F. Hladik & M. J. McElrath. Setting the stage - HIV host invasion. Nature Reviews Immunology 2008, 8:447-457.
F. Hladik, P. Sakchalathorn, L. Ballweber, G. Lentz, M. Fialkow, D. Eschenbach, M. J. McElrath. Initial events in establishing vaginal HIV-1 entry and infection. Immunity 2007, 26:257-70 and cover image.
J. Zhu, D. M. Koelle, J. Cao, J. Vazquez, M. Li Huang, F. Hladik, A. Wald, L. Corey. Virus-specific CD8+ T cells accumulate near sensory nerve endings in genital skin during subclinical HSV-2 reactivation. Journal of Experimental Medicine 2007, 19;204:595-603.
F. Hladik, H. Liu, E. Speelmon, D. Livingston-Rosanoff, S. Wilson, E. Sakchalathorn, Y.Hwangbo, B. Greene, T. Zhu, and M. J. McElrath. Combined effect of CCR5Δ32 heterozygosity and CCR5 promoter polymorphism A -2459G on CCR5 expression and resistance to sexual HIV-1 transmission. Journal of Virology 2005, 79:11677-11684.
F. Hladik, A. Desbien, J. Lang, L. Wang, Y. Ding, S. Holte, A. Wilson, J. Xu, M. Moerbe, S. Schmechel, and M. J. McElrath. Most highly exposed seronegative men lack HIV-1-specific, IFN-γ secreting T cells. Journal of Immunology 2003, 171:2671-2683.
F. Hladik, S. Bender, R. E. Akridge, Y. X. Hu, C. Galloway, D. Francis, M. J. McElrath. Recombinant HIV-1 glycoprotein 120 induces distinct types of delayed hypersensitivity in persons with or without pre-existing immunologic memory. Journal of Immunology
F. Hladik, G. Lentz, R. E. Akridge, G. Peterson, H. Kelley, A. McElroy, M. J. McElrath. Dendritic cell/T cell interactions support co-receptor independent HIV-1 transmission in the human genital tract. Journal of Virology 1999, 73:5833-5842.
See at http://www.cell.com/Cell_Picture_Show-immunology how Langerhans cells in the human vagina