Model Development

Model refinement in Pigtailed Macaques

Dorothy L Patton, PhD, Principal Investigator

The objective of the proposed studies is to develop new protocols and/or, modify existing protocols that are designed to evaluate safety and efficacy to further investigate the effectiveness of newly developed MPTs or other therapeutics in the preclinical pigtailed macaque animal model. The pigtailed macaque is an ideal model for studying the female reproductive tract in that the menstrual cycle is similar to that of human females in length and pattern, and the species is naturally susceptible to these human STIs without the need for exogenous hormone treatments.

Funding Source: NIH/National Institutes of Allergy and Infectious Diseases/ BIOQUAL
End Date: 4-30-17

Development of nonhuman primate model of S. aureus nasal carriage

Dorothy L Patton, PhD, Principal Investigator

Currently small animal models of nasal carriage of S. aureus are less than relevant to the human condition. Therefore it is scientifically important to establish a nonhuman primate model as this will likely be the closest to human nasal colonization. The Patton Laboratory has recently found that the pigtailed macaque is naturally colonized by S. aureus in their noses. In the first aim we will colonize pigtailed macaques with isolates of S. aureus relevant to the human nose and clinical infections. In the second aim, we will explore how SA can affect the innate nasal host defense by examining changes in the expression of mucosal factors that occur during experimental nasal SA colonization in these monkeys. These studies will allow for the development of a useful model of SA nasal carriage in macaques, as well as determining how successive recolonization of SA affects macaque nasal microflora and mucosa.

Funding Source: University of Central Florida (NIH)
End Date: 6-30-20