Schedule for P450 Conference

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Date Time Event Speakers Session Chair
1:00 - 6:00 Registration Opens    
  3:45-4:00 Introductory Comments   Allan Rettie and William Atkins
  4:00-5:00 The Bill Peterson Memorial Plenary Lecture "Bill Peterson and the Reaction ‘Wheel’ of Cytochrome P450"
Stephen G. Sligar,
University of Illinois
  5:00-7:00 Welcome Reception    
9:00-noon Session 1 - Cytochrome P450 Gene Regulation 9:00 - 9:35 a.m.
Roland Wolf
University of Dundee
"Defining Functions and Electron Transfer Pathways of the P-450 System in vivo: Studies with Knockout and Humanized Models"

9:35 - 10:10 a.m.
Masahiko Negishi
"Epidermal Growth Factor Receptor: A Phenobarbital Receptor to Activate CAR and CYP Genes"

Morning Break: 10:10 - 10:30 a.m.

Abstract talk 10:30 - 10:50 a.m.
Shigeyuki Uno
Nihon University
"Coordinate Expression of P450 Enzymes by Aryl Hydrocarbon Receptor and Vitamin D Receptor"

Abstract talk 10:50 - 11:10 a.m.
Ulrich Zanger
University of Tuebingen
"Adme-Related Micrornas in Human Liver: Expression, Variability and Association with Drug Metabolizing Cytochromes P450"

Abstract talk 11:10 - 11:30 a.m.
Alan Anderson
Université Laval
"Induction of CYP2B Genes by Phenobarbital and Dexamethasone: Two Inducers and Two Mechanisms

11:30 - 12:00 p.m.
Frank J. Gonzalez
"PPARα and Hepatic Oxidative Stress Activate the GADD45B Gene Via Degradation of the Transcriptional Repressor STAT3"
Masahiko Negishi, NIEHS

Frank J. Gonzalez, NCI
  9:00-noon Session 2 - Catalytic Mechanisms of Cytochrome P450s 9:00 - 9:35 a.m.
William Atkins
University of Washington
"Functional and Spectroscopic Effects of Ligand Interactions with Axial Water in Cytochrome P450s"

9:35 - 10:10 a.m.
James DeVoss
University of Queensland
"Mechanistic Insights from Bacterial P450 Catalyzed Oxidations"

Morning Break: 10:10 - 10:30 a.m.

10:30 - 11:05 a.m.
John Groves
Princeton University
"C-H Hydroxylation Mechanisms of Novel Heme-Thiolate Proteins: What They Tell Us About Cytochrome P450"

11:05 - 11:40 a.m.
John C. Hackett
Virginia Commonwealth University
"Mechanisms of P450-Catalyzed Deformylation"

11:40 - 12:10 a.m.
Paul R. Ortiz de Montellano
UC San Francisco
"Mechanism of CYP1A2-Catalyzed Carbon-Carbon Bond Cleavage in Nabumetone Activation"
Paul Ortiz de Montellano, UC San Francisco

John Groves, Princeton University
  Noon-1:30 Buffet Lunch    
  1:30-4:30 Session 3 - Cytochrome P450-Dependent Drug Metabolism 1:30 - 2:05 p.m.
Kent Kunze
University of Washington
"Non-Dissociative Sequential Metabolism of Alkylamines by P450 Enzymes"

2:05 - 2:40 p.m.
Hiroshi Yamazaki
Showa Pharmaceutical University
"Drug Oxidations and Interactions Mediated by Monkey and Human Cytochrome P450 Enzymes"

Afternoon Break: 2:40 - 3:00 p.m.

3:00 - 3:35 p.m.
Amit Kalgutkar
"Metabolism-Guided Drug Design: Emphasis on Bioactivation"

3:35 - 4:10 p.m.
Kaushik Mitra
"Identifying and Eliminating Biotransformation Liabilities Early in Drug Discovery"

Abstract talk 4:10 - 4:30 p.m.
Kathrin Klein
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology
"Novel CYP2B6 Polymorphisms and Their Impact on Efavirenz and Bupropion Metabolism"
Larry Wienkers, Amgen

Kent Kunze, University of Washington
  1:30-4:30 Session 4 - Nanotechnology and Cytochrome P450 Biophysics 1:30 - 2:05 p.m.
Teruyuki Nagamune
University of Tokyo
"Artificial Self-Sufficient Bacterial P450 System with PCNA as a Scaffold"

2:05 - 2:40 p.m.
James Kincaid
Marquette University
"Probing Reactive Intermediates of Nanodisc-Associated Cytochromes P450 with Resonance Raman Spectroscopy"

Afternoon Break: 2:40 - 3:00 p.m.

3:00 - 3:35 p.m.
Thomas Pochapsky
Brandeis University
"‘Are you my substrate?’ NMR Investigations of Substrate Recognition in Cytochromes P450"

3:35 - 4:10 p.m.
John Dawson
University of South Carolina
"Spectral Models for Cytochrome P450 Based on the H93G Myoglobin Cavity Mutant"

Abstract talk 4:10 - 4:30 p.m.
Lisa Martin
Monash University
"The Role of Dimerization and Protein-Protein Interactions for P450 Aromatase (CYP19) Proteins"
Andrew Munro,
University of Manchester

David Leys,
University of Manchester
  4:30-6:30 Poster Session A    
9:00-noon Session 5 - Structural Biology of Cytochrome P450s 9:00 - 9:35 a.m.
Emily Scott
University of Kansas
"Cytochrome P450 17A1 Interactions with Substrates and Cytochrome b5"

9:35 - 10:10 a.m.
James Halpert
UC San Diego
"Structural Determinants of Drug and Hydrocarbon Binding to P450 2B Enzymes"

Morning Break: 10:10 - 10:30 a.m.

10:30 - 11:05 a.m.
Eric Johnson
Scripps Research Institute
"Probing P450 2D6 Structure Function Relationships Using X-Ray Crystallography"

11:05 - 11:40 a.m.
David Leys
University of Manchester
"Small Mutations, Big Effects: Altering the P450 BM3 Conformational Landscapes"

Abstract talk 11:40 - 12:00 p.m.
Yarrow Madrona
UC Irvine
"P450CIN Water Plays a Role in Coupling and Substrate-Binding"
Emily Scott,
University of Kansas

Jim Halpert,
UC San Diego
  9:00-noon Session 6 - Protein-Protein Interactions and Cytochrome P450Ss 9:00 - 9:35 a.m.
Thomas Poulos
UC Irvine
"Crystal Structure of the P450 CAM-PDX Complex"

9:35 - 10:10 a.m.
Marcellus Ubbink
Universitat Leiden
"Biophysical Characterization of the Complex of Cytochrome P450CAM and Putidaredoxin"

Morning Break: 10:10 - 10:30 a.m.

10:30 - 11:05 a.m.
Sheila Sadeghi
University of Torino
"Application of Chimeric Human P450 Enzymes: Drug-Drug and Drug-Food Interactions"

11:05 - 11:40 a.m.
Lucy Waskell
University of Michigan
"Characterization of Cytochrome P450 Reductase Mutants that Regulate the Formation and Stability of the FMN Semiquinone"

11:40 - 12:10 p.m.
Satya Panda
University of Texas Health Sciences San Antonio
"Conditional Deletion of Cytochrome P450 Reductase in Osteoprogenitor Cells in Mice Recapitulates Antley-Bixler Syndrome"
Rita Bernhardt, Universität des Saarlandes

Lucy Waskell, University of Michigan
  12:00 noon Box Lunch Distribution    
  1:00 p.m. Afternoon Excursions    
9:00-noon Session 7 - Steroidogenic/ Sterol-Metabolizing P450s 9:00 - 9:35 a.m.
Marion Sewer
UC San Diego
"Signaling Pathways Regulating Steroid Hydroxylase Gene Expression"

9:35 - 10:10 a.m.
F. Peter Guengerich
Vanderbilt University
"Zebrafish Cytochrome P450 17A2: Structure, Function, and Relevance to other Cytochrome P450s Catalyzing Steroid 17-Oxidations"

Morning Break: 10:10 - 10:30 a.m.

10:30 - 11:05 a.m.
Evan Simpson
Prince Henry's Institute of Medical Research
"Aromatase Cytochrome P450 (CYP19A1) in Obesity and Breast Cancer: Role of Inflammation and Dysregulated Metabolism"

11:05 - 11:40 a.m.
Amit Pandey
University Children's Hospital, Bern
"Human Disorders of Steroidogenic P450s"

Abstract talk 11:40 - 12:00 p.m.
Elaine Tieu
University of Western Australia
"Kinetic Analysis of the Vitamin D3 C24-Oxidation Pathway Catalysed by Human CYP24A1"
Irina Pikuleva, Case Western Reserve

Marion Sewer,
UC San Diego
  9:00-noon Session 8 - Non-CYP Heme Proteins 9:00 - 9:35 a.m.
Yoshitsugu Shiro
Harima Institute
"Fungal and Bacterial Nitric Oxide Reductases: Structures and Functions"

9:35 - 10:10
CS Raman
University of Maryland-Baltimore
"Functional Repurposing of the Cytochrome P450 Scaffold"

Morning Break: 10:10 - 10:30 a.m.

10:30 - 11:05 a.m.
Carrie Wilmot
University of Minnesota
"MauG Catalysis: A Tale of Ferryl Iron, Radicals, and Long Distance Hopping"

11:05 - 11:40 a.m.
Syun-ru Yeh
Albert Einstein College of Medicine
"Tryptophan Dioxygenation by Heme-Containing Dioxygenases - from Stucture to Function"

Abstract talk 11:40 - 12:00 p.m.
Martin Hofrichter
TU Dresden
"Insights into Catalysis, Structure and Phylogeny of Fungal Peroxygenases"
Tom Poulos,
UC Irvine
  Noon-1:30 Poster Session B / Buffet Lunch    
  1:30-4:30 Session 9 - Cytochrome P450s and Drug Design 1:30 - 2:05 p.m.
Galina Lepesheva
Vanderbilt University
"Use of Structure/Function for Design of Drugs to Inhibit CYP51"

2:05 - 2:40 p.m.
Irina Pikuleva
Case Western Reserve University
"Co-crystallization of CYP46A1 with Different Drugs: Interactions in the Active Site and Beyond"

Afternoon Break: 2:40 - 3:00 p.m.

3:00 - 3:35 p.m.
Andrew Munro
University of Manchester
"Fragment Screening Strategies for Mycobacterium Tuberculosis P450 Inhibitor Development"

3:35 - 4:10 p.m.
John R. Falck
University of Texas Southwestern
"Role of Endogenous CYP Fatty Acid Metabolites and Clinical Applications"

4:10 - 4:30 p.m.
Matthew A. Hulverson
University of Washington
"CYP51 Inhibitors Derived from an Anti-Cancer Drug Lead to Treat Chagas Disease"
Michael Waterman
Vanderbilt University
  1:30-4:30 Session 10 - Cytochrome P450 Biotechnology and Protein Engineering 1:30 - 2:05 p.m.
Vlada Urlacher
Universität Dusseldorf
"The Challenge of Designing P450-based Biocatalysts: From Electron Transfer to Enzyme Selectivity"

2:05 - 2:40 p.m.
John McIntosh
California Institute of Technology
"Formal Nitrene and Carbene Transfers Catalyzed by Engineered Cytochrome P450 Enzymes"

Afternoon Break: 2:40 - 3:00 p.m.

3:00 - 3:35 p.m.
Frank Hannemann
Universität des Saarlandes
"Recombinant P450 Systems for Whole-Cell Biocatalysis of Hydrophobic Substrates"

3:35 - 4:10 p.m.
Toshiyuki Sakaki
Toyama Prefectural University
"Possible Application of Bacterial and Mammalian Vitamin D Hydroxylases"

Abstract talk 4:10 - 4:30 p.m.
Paul Kelly
Manchester Institute of Biotechnology
"Engineering Hybrid P450-Reductase Fusions for Biocatalysis"
Toshiyuki Sakaki, Toyama Prefectural University

Vlada Urlacher, Universität Dusseldorf
  5:30 p.m. Conference Dinner    
10:00-11:20 Selected Poster Presentations   Rheem Totah, University of Washington
  11:20-11:30 Presentation of Tabor Award   Fred Guengerich
  11:30-1:00 Box Lunch    
  1:00-4:00 Session 11 - P450 Bioactivation, Covalent Adduction, and Enzyme Processing 1:00 - 1:35 p.m.
Almira Correia
UC San Francisco
"Cytochromes P450 Chained and Fettered: The Road to Perdition"

1:35 - 2:10 p.m.
Paul Hollenberg
University of Michigan
"Mechanism-Based Inactivators of Cytochromes P450 as Probes of Structure and Function"

Afternoon Break: 2:10 - 2:30 p.m.

2:30 - 3:05 p.m.
Christopher Reilly
University of Utah
"Inhibition of CYP3A Enzymes by Fluticasone Propionate: Impact on Pharmacotherapy"

3:05 - 3:40 p.m.
Dan Rock
Amgen, Inc.
"The Roles of P450 in Drug Bioactivation and Exposure to Reactive Metabolites"

Abstract talk 3:40 - 4:00 p.m.
Brian Baer
Array BioPharma, Inc.
"A Reactive 3-OXO-Pyrazolopyrimidinium Cation Intermediate Formed by Cytochrome P450-Mediated Bioactivation of a 3-Urea-Substituted Pyrazolopyrimidine Can be Deactivated by Subsequent Reduction and Glucuronidation"
Paul Hollenberg, University of Michigan

Allan Rettie, University of Washington
  1:00-4:00 Session 12 - Heme Chemistry/ Spectroscopy 1:00 - 1:35 p.m.
Ilia Denisov
University of Illinois
"P450 Kinetics in Slow Motion Monitored by Cryospectroscopy"

1:35 - 2:10 p.m.
Paul Champion
Northeastern University
"Investigations of Heme Ligation and Ligand Switching in Cytochrome P420"

Afternoon Break: 2:10 - 2:30 p.m.

2:30 - 3:05 p.m.
Lionel Cheruzel
San Jose State University
"Light-Driven CYP102A1 Biocatalysts with High Catalytic Activity"

3:05 - 3:40 p.m.
Mike Green
Pennsylvania State University
"Understanding C-H Bond Activation in Cytochrome P450"

Abstract talk 3:40 - 4:00 p.m.
Daniel Collins
University of South Carolina
"The Peroxide Shunt Reaction of Ferric Cytochrome P450-CAM with Substituted Perbenzoic Acids: A Model for the O-O Bond Cleavage Step Leading to Compound I"
John Dawson, University of South Carolina
  4:00-4:30 Closing Remarks   Allan Rettie and William Atkins


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