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PBIO SEMINAR SERIES

Location: HSB G-328, 10:30am (unless otherwise noted)

January 30, 2014 - 2013-2014 SEMINAR SERIES

"Microtubule Dynamic Instability: Molecular Mechanisms Revealed by In Vitro Reconstitution"

Marija Zanic, Yale University

Host: Stan Froehner

Microtubules are dynamic cytoskeletal polymers essential for cell division, motility, shape and intracellular transport. Remodeling of the microtubule cytoskeleton relies on precise regulation of switching between growth and shrinkage of individual polymers, behavior known as microtubule dynamic instability. I use bottom-up in vitro reconstitution approaches with purified proteins and total-internal-reflection-fluorescence microscopy to elucidate the molecular mechanisms underlying dynamic instability and its regulation. My research showed that microtubules age: the switch between growth and shrinkage requires multiple steps, allowing for greater control of microtubule length distributions. Additionally, I identified a minimal system needed for reconstitution of physiological microtubule growth rates, never previously obtained using purified components. The acceleration of growth is achieved through collective effects of two microtubule-regulating proteins, leading to fast and dynamic microtubule behavior typically observed in cells.


Alt Time: 10:30 a.m.
Alt Location: HSB G-328