Physiology and Biophysics

Seminar Archives

‘2015-2016’ and ‘2016-2017’ Seminar Series

Oct
1
Thu
2015
Alex Huk, PhD, UT-Austin @ HSB G-328
Oct 1 @ 9:30 am – 10:30 am

Motion processing in the primate dorsal stream

Short summary: In this talk I will present the dorsal stream of primates as a model system for understanding rich aspects of sensorimotor integration and neural coding. Building off classic work that has characterized the encoding of frontoparallel visual motion underlying the perception of simple forms off motion, I will describe our work attempting to learn more about how the brain extracts 3-dimentional velocities, forms perceptual decisions, and how it functions in free-viewing contexts that do not require training or conventional threshold-level tasks.

Huk Vision / Decision Lab

host: Greg Horwitz

Oct
15
Thu
2015
Mike Manookin, PhD, University of Washington @ HSB G-328
Oct 15 @ 9:30 am – 10:30 am

Distinctive biophysical and light-encoding properties of inhibitory neurons in the macaque monkey retina.

Abstract:
The retina is the only part of the brain that is visible to the naked eye.  It’s natural isolation from the rest of the brain makes the retina an ideal model for studying the biophysical properties of neurons and the computational properties of neural circuits in an intact experimental preparation.  In this talk, I will discuss the biophysical and light-encoding properties of an inhibitory neuron—the wiry-type amacrine cell.  These cells have been identified morphologically in the macaque and human retina.  They exhibit long, thin dendritic processes that exhibit regenerative potentials likely arising from NMDA spikes.  In addition, these cells show asymmetrical responses to visual motion, suggesting that they contribute to motion processing in the primate visual stream.

website

host: Stan Froehner

Oct
22
Thu
2015
Jeffrey Holt, PhD, Harvard @ HSB G-328
Oct 22 @ 9:30 am – 10:30 am

TMC function, dysfunction and the prospects for inner ear gene therapy

 

Jeffrey R. Holt, Ph.D.
Associate Professor
Department of Otolaryngology
Harvard Medical School

Holt/Geleoc Lab

abstract:

TMC proteins are of considerable interest for basic inner ear biologists and for translational and clinical neuroscientists because they cause deafness in mice and humans when mutated.  Our research group has proposed they may be components of the elusive mechanotransduction channel in sensory hair cells.  Evidence for and against this hypothesis will be presented.  In addition, a potential gene therapy approach to restore hair cell and auditory function in mice and humans with Tmc1 mutations will be discussed.

host: Ed Rubel

Nov
12
Thu
2015
Kurt Haas, UBC @ HSB G-328
Nov 12 @ 9:30 am – 10:30 am

Experience-driven brain circuit remodeling 

The spatial arrangement of a neuron’s synapses determines how inputs interact to perform computations, such as through recruitment of nonlinear conductances by spatially clustered activity. However, it remains poorly understood how such functional arrangements arise. We developed a random access microscope able to simultaneously record activity of every excitatory synapse, somatic firing, and dendrite morphology of an individual neuron throughout plasticity-inducing visual training in awake animals. We find that dendrite growth and pruning in the developing retinotectal system of transparent Xenopus tadpoles is regulated by sensory experience in a manner strongly dependent on each neuron’s evoked responses. We identify rules based on local dendritic activity patterns that promote clustering of synaptic inputs with shared tuning and promote processing of the specific stimuli experienced. 

Kurt Haas, Ph.D.

Assistant Professor
Brain Research Centre

Department of Cellular and
Physiological Sciences

University of British Columbia

lab website: http://www.haaslab.com/

Host: Andres Barria

Dec
3
Thu
2015
Henry Markram, PhD, Ecole Polytechnique Federale de Lausanne @ HSB G-328
Dec 3 @ 9:30 am – 10:30 am

Reconstruction and Simulation of Neocortical Microcircuitry

Host: Eb Fetz

Dec
17
Thu
2015
Miriam B. Goodman, PhD, Stanford @ HSB G-328
Dec 17 @ 9:30 am – 10:30 am

Miriam B. Goodman, Ph.D.

Stanford University
Associate Professor of Molecular and Cellular Physiology and, by courtesy, of Mechanical Engineering

seminar title: TBD

Lab: http://wormsense.stanford.edu/

Jan
14
Thu
2016
Rizal Hariadi, PhD, Harvard University @ HSB G-328
Jan 14 @ 9:30 am – 10:30 am

Tuning collective protein interactions using programmable DNA nanostructures

The emergence of collective movement, as in stellar streaming, flocks of birds, and ant colonies, is a widespread phenomenon across all scales. At the cellular level, the collective movement of myosins motors drives cell division, membrane trafficking, and muscle contraction. Understanding collective movements of myosin motors from whole-cell  systems is challenging because the overly-complex cellular environment obscures the underlying interactions governing the higher order functions.
To systematically dissect cellular dynamics of  motor proteins, we engineered two model systems consisting of programmable DNA nanostructures, patterned with precise numbers, types, and spatial arrangements of purified myosin motors. First, we use rectangular DNA origami nanostructures to dissect the tug-of-war in motor protein ensembles with opposite polarity myosin motors. Second, we use DNA nanotubes as platforms for engineering artificial thick filaments, the basic contractile unit of muscle, to reveal the behavior of a large number of myosin motors. Beyond controlling the spatial arrangement of myosin motors, we use DNA nanotubes as mechanical sensors for forces at short length scales and fast time scales that would be difficult to investigate by other means. These studies uncover elegant engineering principles for designing force sensors and molecular transporters on complex landscapes. Further, these DNA nanostructures can serve as platforms to reconstitute other emergent systems in molecular biology and far-from-equilibrium systems in soft condensed matter physics.
Finally, I will conclude with a discussion on the transformative potential and future directions, including the biological physics of malaria parasite invasion and mechanically active nanostructures for solving protein structures under defined tension.
Rizal F. Hariadi, Ph.D.
Wyss Institute for Biologically Inspired Engineering at Harvard University

Chalk talk, Friday, January 15th, at 9:30 in G-417.

Jan
21
Thu
2016
Rose E. Dixon, PhD, University of Washington @ HSB G-328
Jan 21 @ 9:30 am – 10:30 am

L-type Ca2+ Channel Oligomerization in Hearts and Minds

In ventricular myocytes, excitation-contraction (EC) coupling occurs via a Ca2+ induced Ca2+ release mechanism such that the depolarization created by an action potential (AP) triggers Ca2+ influx through voltage-dependent L-type Ca2+ channels (i.e., Cav1.2 channels), which in turn stimulates further Ca2+ release from ryanodine receptors (RyRs) on the nearby junctional sarcoplasmic reticulum. The simultaneous activation of multiple RyRs across the myocyte results in a cell-wide increase in intracellular Ca2+ and triggers contraction. This process is remarkably reproducible despite the confounding fact that, at the membrane potential reached during the AP plateau, the driving force for Ca2+ entry through a single Cav1.2 channel is not sufficient to reliably activate RyRs. Instead it has been proposed that up to ten Cav1.2 channels must open simultaneously during the AP plateau to achieve this coupling fidelity. The mechanism that permits coordinated opening of multiple Cav1.2 channels during EC-coupling, however, has not yet been elucidated. Using electrophysiological, and optical approaches, we have found that an allosteric interaction between the C-terminal domains of voltage-gated CaV1.2 channels induces an increase in the open probability of the channels that amplifies Ca2+ influx during the AP and contributes to reproducible EC-coupling. We have also discovered that Cav1.3 channels that regulate excitability in neurons often display the same cooperative behavior. The objectives of this presentation are to summarize the molecular details of the channel interactions that we have resolved thus far and to highlight the significance of these findings to the cardiac and neuronal field

Rose Dixon, Ph.D.

University of Washington

 

Chalk talk, Friday, January 22nd, at 9:30 in G-417.

Jan
28
Thu
2016
Ivana Yih-Tsue Kuo, PhD, Yale University @ HSB G-328
Jan 28 @ 9:30 am – 10:30 am

Where the kidney meets the heart: calcium signaling, cardiomyopathies and polycystic kidney disease

Polycystin 2 mutations lead to polycystic kidney disease (PKD), a condition with no cure. Although cysts are the defining characteristic of PKD, cardiac dysfunction is the main cause of mortality, however, the underlying reasons are not known. Polycystin 2 acts both as an intracellular calcium channel and a modulator to alter intracellular signaling pathways. I will present evidence demonstrating that perturbations to calcium signaling result in cysts. Second, I will show that mutations and decreased availability of polycystin 2 results in cardiac dysfunction. Understanding the molecular actions of polycystin 2 in multiple tissue settings will help direct therapeutic efforts into treating PKD.

Ivana Yih-Tsue Kuo, PhD,

Yale University

Chalk talk, Friday, January 29th, at 9:30 in G-417.

Feb
4
Thu
2016
Andy J. Chang, PhD, Stanford @ HSB G-328
Feb 4 @ 9:30 am – 10:30 am

A molecular mechanism of acute oxygen sensing

While the molecular mechanism for slower adaptations to low oxygen (hypoxia) is known, mechanisms for rapid oxygen sensing are not well understood.  Located at the bifurcation of the carotid artery in the neck, the mammalian carotid body is a chemosensory organ that senses decreases in blood oxygen to stimulate breathing within seconds.  I will present the identification of an acute oxygen-sensing pathway in the mouse carotid body that senses hypoxia indirectly through detection of a metabolite.

Andy J. Chang, PhD

Stanford University

 

Chalk Talk on Friday, February 5th, 9:30am in G-417. 

Mar
10
Thu
2016
Gary Brouhard, PhD., McGill University @ HSB G-328
Mar 10 @ 9:30 am – 10:30 am

Seminar Title:  Thoughts (and Experiments) on Microtubule Nucleation

Abstract:   Microtubules are born and reborn continuously, even during quiescence. These polymers are nucleated from templates, namely γ-tubulin ring complexes (γ-TuRCs) and severed microtubule ends. Interestingly, the rate of microtubule nucleation increases as cells enter mitosis, and all cells nucleate microtubules in distinct regions of their cytoplasms. How are these spatial and temporal profiles for microtubule nucleation established? I will discuss my lab’s recent attempts to answer this question using a mix of single-molecule biophysics, cell biology, and structural biology.

Host:  Chip Asbury

Apr
14
Thu
2016
Vanessa Ruta, Rockefeller University @ HSB G-328
Apr 14 @ 9:30 am – 10:30 am

Circuit Mechanisms for Flexible Sensory Processing

In a complex and dynamic environment, animals must constantly vary their behavior to accommodate changing circumstances and contingencies. Yet, how associative brain centers flexibly couple the same sensory input to alternative behavioral pathways remains unclear. My lab takes advantage of the relative simplicity 
of the Drosophila olfactory system to gain insight into the synaptic and circuit mechanisms through which context and experience can modify odor processing.
 In Drosophila, the mushroom body is a higher brain center that integrates olfactory and neuromodulatory reinforcement signals to generate learned and adaptive behaviors. I will describe recent work in which we used functional synaptic imaging and electrophysiology to show that the mushroom body functions like a switchboard in which dopaminergic neuromodulation can reroute the same odor signals to different behavioral circuits depending on the state and experience of the fly. Our data suggest a circuit mechanism for behavioral flexibility in which neuromodulatory networks act with exquisite spatial precision to transform a single sensory input into different patterns of output activity.

Vanessa Ruta, Ph.D.

Gabrielle H. Reem and Herbert J. Kayden Assistant Professor
Laboratory of Neurophysiology and Behavior

Rockefeller University

 

Host: John Tuthill

Apr
28
Thu
2016
James Trimmer @ HSB G-328
Apr 28 @ 9:30 am – 10:30 am
Ion Channels and Their Specialized Membrane Domains

The restricted and dynamically regulated subcellular localization of signaling proteins in highly differentiated cells such as mammalian neurons defines intra- and inter-cellular signaling events.  Among neuronal proteins exhibiting the most highly compartmentalized expression are ion channels, whose localization at specific sites can both define signaling in that neuronal compartment, but also allow for discrete regulation of the population of channels found at these sites. I will describe the diverse mechanisms that establish, maintain and dynamically regulate ion channel expression at specific sites to support neuronal function and confer plasticity to neuronal signaling.

James Trimmer, Ph.D.

UC Davis, Departments of Physiology and Membrane Biology and Neurobiology, Physiology and Behavior

 

host: Nicholas Poolos

May
12
Thu
2016
2016 Hille Lecture, Catherine Dulac, PhD, Harvard @ HSB T-625
May 12 @ 4:00 pm – 5:00 pm
“Molecular and Neural Architecture of Social Behavior Circuits in the Mouse.”

Catherine Dulac, PhD,

Higgins Professor of Molecular and Cellular Biology

Howard Hughes Medical Institute Investigator

Harvard University

host: Stan Froehner

May
26
Thu
2016
Andrea Meredith, PhD, University of Maryland @ HSB G-328
May 26 @ 9:30 am – 10:30 am

Big Time for BK: Mechanisms of Circadian Rhythm in Neuronal Activity

Andrea L. Meredith Ph.D.
Associate Professor
University of Maryland, Physiology

Meredith Lab

host: Sharona Gordon

seminar abstract:

Inactivation gating is an intrinsic property of several types of voltage-dependent ion channels, closing the conduction pathway during membrane depolarization and dynamically regulating neuronal activity. BK large conductance voltage- and Ca2+-activated K+ channels undergo N-type inactivation via their β2 subunit, but the physiological significance has not been clear. To understand the role of BK channel inactivation in neuronal excitability, we identified a circuit where β2 is expressed and where dynamic regulation of the BK current is critical for neural coding, the suprachiasmatic nucleus (SCN) ‘clock circuit’ of the hypothalamus. BK channel regulation of SCN action potentials is dynamic, with a significant effect on nighttime firing, but little effect during the day. Correlated with this dynamic role in SCN excitability, we found that inactivating BK currents predominate during the day, reducing steady-state current levels. At night inactivation is diminished, resulting in larger BK currents. Loss of β2 eliminates BK channel inactivation, abolishing the diurnal variation in both BK current magnitude and SCN firing, and disrupting behavioral rhythmicity. Selective restoration of inactivation via the isolated β2 N-terminal ‘ball-and-chain’ domain rescues BK current levels and firing rate, unexpectedly contributing to the sub-threshold membrane properties that shift SCN neurons into the daytime ‘upstate’. These findings reveal that the intrinsic clock employs inactivation gating as a biophysical switch to set the diurnal variation in SCN excitability that underlies circadian rhythm.

Jun
9
Thu
2016
2016 Crill Lecture, Mark Goldman, PhD, UC Davis @ T-435, H.S.B.
Jun 9 @ 3:30 pm – 4:30 pm
2016 Wayne E. Crill Lecture
“Microcircuits for short-term memory storage and neural integration”
Time: 3:30 pm
Location: T-435, H.S.B.
Mark Goldman
Professor of Neurobiology, Physiology, and and Behavior
UC Davis
Jun
16
Thu
2016
James W. Grau PhD, Texas A&M @ HSB G-328
Jun 16 @ 9:30 am – 10:30 am

Exploring the nature of spinal cord plasticity: Neurobiological mechanisms and implications for recovery after injury

Prior research has shown that pain (nociceptive) circuits within the spinal cord are affected by environmental relations and support some simple forms of learning (e.g., sensitization, instrumental conditioning). Further, learning can induce a modification in the capacity for learning (a form of metaplasticity); controllable stimulation enables learning through a process that depends upon brain derived neurotrophic factor (BDNF) while uncontrollable stimulation induces a lasting learning impairment that has been linked to the development of central sensitization and the cytokine tumor necrosis factor (TNF). I review data that show temporal predictability also affects spinal function by engaging an internal oscillator. Spinal injury disrupts descending serotonergic fibers that appear to quell nociceptive sensitization. This process is related to a change in the co-transporters that regulate spinal GABA function. It is also shown that nociceptive stimulation impairs recovery after a contusion injury. This effect is related to increased cell death and alterations in BDNF/TNF. Potential therapeutic treatments will be discussed.

 

 

Mary Tucker Currie Professor,
Department of Psychology and Neuroscience
Texas A&M University
host: Nik Dembrow
Sep
29
Thu
2016
PBIO Seminar Series: Andrea Wills @ HSB G-328
Sep 29 @ 9:30 am – 10:30 am
“Remodeling transcription and the chromatin landscape during vertebrate regeneration”

Andrea Wills, PhD

Assistant Professor of Biochemistry, University of Washington

Abstract: Humans have a limited capacity to regenerate complex differentiated tissues. In contrast, many amphibians are readily able to regenerate muscle, spinal cords, eyes and even entire limbs. Among amphibians, frogs such as Xenopus tropicalis occupy a uniquely powerful position as a regenerative system, because they can regenerate all of these tissues well as tadpoles, but lose this competence as they undergo metamorphosis. Our goal is to identify the molecular mechanisms that enable differentiated tissues to regenerate in tadpoles but are restricted in adults. In pursuit of this goal, our particular interest is in understanding the gene regulatory landscape that underlies transcriptional activation of new gene products during regeneration. While many individual genes have been implicated in specific regenerative processes, a comprehensive understanding of how the transcriptome and chromatin environment are remodeled during regeneration has been lacking. We have taken an integrated genomic approach during X. tropicalis tail regeneration to understand the dynamic changes that occur in gene expression and the underlying chromatin landscape. Using this combined approach, our research has uncovered new categories of gene expression that are prioritized early in regeneration, as well as evidence that chromatin is rapidly remodeled throughout the nucleus in response to amputation. These discoveries provide new insights into the molecular mechanisms that govern regenerative responses in complex vertebrate tissues.

host: Greg Horwitz

Oct
20
Thu
2016
PBIO Seminar Series: Jeffrey Ruberti @ HSB G-328
Oct 20 @ 9:30 am – 10:30 am

How are load-bearing soft tissues built?

Force-structure causality in the matrix

Jeffrey W. Ruberti
Professor, Bioengineering
Northeastern University

host: Chip Asbury

 

Abstract: Shockingly, there exists no established model that can explain how a cluster of vertebrate cells which are expressing matrix proteins manage to produce, refine and grow load-bearing structures that are organized over much longer length scales than the cells themselves. The current best guess model, which was proposed in the mid-1980s by David Birk and Robert Trelstad (subsequently carried forward by Karl Kadler’s group) suggests that cells extrude formed collagen fibrils into the extracellular space via structures which are termed “fibripositors”. Thus, one imagines that the cells, working together, somehow weave the collagen into the matrix, thread by thread with the necessary exposed loose ends finding each other and fusing to form long-range, organized connective tissue. However, gathering evidence to support this model is severely hampered because it is nearly impossible to observe cells in the act of producing matrix while observing the collagen fibril deposition directly at the nanoscale (but we sure are trying to do that in the lab). In addition, the fibripositor model does not contemplate either matrix refinement or growth. Thus, we are not only in need of experimental evidence to support the fibripositor theory, we are short of a comprehensive testable hypothesis in general for how tissue is built. To address this dearth, we have chosen to make a simple (and risky) assumption: We reject the idea that the cells directly manipulate individual collagen monomers or fibrils to make tissue. Instead, we assume the cell has spent much of its time (~billion years) refining specific molecular systems (secretomes) that are designed to “settle” into their appropriate configuration simply by “reading” the energetic landscape. To generate load-bearing connective tissues, we suggest that the cells provide appropriate geometry by self-organizing and then produce an appropriate secretome that has been designed to assemble in opposition to the locally and globally applied mechanical forces which threaten to dissipate animal structure. In effect, we predict that mechanical strain is actually a long-range structure producing signal which works via mechanical allostery to modulate both collagen fibril assembly and retention. Because collagen is generally found resisting tension in load-bearing soft-connective tissue, we expect that the mechanical environment directly shifts the molecular energetics such that collagen’s inherent stability and assembly kinetics are enhanced in the direction of applied tensile forces. I will present the current state and limitations of our investigation of this risky assumption and entertain your thoughts, concerns and comments.

 

Oct
27
Thu
2016
PBIO Seminar Series: Samuel J. Sober PhD @ HSB G-328
Oct 27 @ 9:30 am – 10:30 am

Vocal motor control and sensorimotor learning: behavior, neurophysiology, and biomechanics

The brain uses sensory feedback to calibrate the performance of complex behaviors. However, the neural and computational bases of sensorimotor learning remain mysterious. Our lab uses behavioral, physiological, biomechanical, and computational techniques to investigate the biological underpinnings vocal learning in songbirds. My talk will cover three ongoing lines of investigation into how songbirds correct vocal errors and precisely coordinate the acoustics of vocal production. First, our behavioral studies demonstrate that songbirds use vocal variability to constrain the speed and extent of vocal learning, and that the dynamics of learning across a number of experimental conditions can be understood as the result of an iterative process of Bayesian inference. Second, recent behavioral and anatomical studies demonstrate a crucial role for dopaminergic inputs to a basal ganglia nucleus in mediating vocal reinforcement learning. Third, neurophysiological recordings and computational analyses suggest that cortical motor neurons employ a millisecond-resolution spike timing code to regulate vocal behavior. Recent single-unit recordings from muscle tissue in behaving animals and in vitro measures of vocal biomechanics further suggest that millisecond-scale spike timing is an essential component of motor control, suggesting that reorganization of fine temporal spiking patterns might underlie vocal plasticity.

Samuel J. Sober, Ph.D.

Assistant Professor of Biology, Emory University

Host: David Perkel

Nov
3
Thu
2016
PBIO Seminar Series: Fan Wang @ HSB G-328
Nov 3 @ 9:30 am – 10:30 am

Examining neural circuits mediating social behaviors and motor learning using the CANE technology

Fan Wang, Ph.D.Associate Professor,
Department of Neurology
Duke University

 

Short summary:
We developed a new technology called CANE for capturing activated neuronal ensembles to dissect the functions and connectivity of behaviorally activated neurons. Using CANE in combination with optogenetics and imaging, we delineated a hypothalamic social fear circuit, and examined the cortical circuit underlying skilled motor learning.

host: John Tuthill

Nov
10
Thu
2016
PBIO Seminar Series: Gabriela Popescu @ HSB G-328
Nov 10 @ 9:30 am – 10:30 am

NMDA Receptors: learning from singles and beyond

Gabriela Popescu, PhD

ProfessorDepartment of Biochemistry, Jacobs School of Medicine and Biomedical SciencesUniversity of Buffalo

Popescu Laboratory

Abstract

NMDA receptors are members of the tetrameric ionotropic glutamate receptor family that fulfill unique and critical roles during the normal development and function of the central nervous system. These roles are supported by characteristic kinetic attributes of the glutamate-evoked output, which in turn reflect the receptor’s operation. In this talk, I will review the biologically salient features of the NMDA receptor response and describe recent mechanistic insights afforded by kinetic modeling of one channel currents. In addition, I will present unpublished data on novel modalities of gating

host: Andres Barria

Nov
17
Thu
2016
Lamport Lecture – Robert Lefkowitz @ HSB T-625
Nov 17 @ 4:00 pm – 5:00 pm

“Seven Transmembrane Receptors”

Robert J. Lefkowitz, MD

James B. Duke Professor of Medicine

Investigator, Howard Hughes Medical Institute

Duke University Medical Center

Seven transmembrane receptors (7TMRs), also known as G protein coupled receptors (GPCRs) represent by far the largest, most versatile, and most ubiquitous of the several families of plasma membrane receptors. They regulate virtually all known physiological processes in humans. As recently as 40 years ago, the very existence of cellular receptors for drugs and hormones was highly controversial, and there was essentially no direct means of studying these putative molecules. Today, the family of GPCRs is known to number approximately 1,000, and crystal structures have recently been solved of approximately 25 members of the family and even of a receptor-G protein complex. In my lecture, I will briefly review how the field has evolved over the past 40 years, hanging some of the story on my own research throughout this period. Then I will discuss recent developments in the field, which are changing our concepts of how the receptors function and are regulated in fundamental ways. These include the duality of signaling through G-proteins and β-arrestins; the development of “biased ligands”; and the possibility of leveraging this new mechanistic and molecular information to develop new classes of therapeutic agents. Finally, I will discuss recent biophysical and structural studies of receptor-barrestin interactions.

Dec
1
Thu
2016
PBIO Seminar Series: Dwight Bergles @ HSB G-328
Dec 1 @ 9:30 am – 10:30 am

Dwight Bergles, Ph.D.
Professor
The Solomon H. Snyder Department of Neuroscience
Johns Hopkins University
Website: http://www.bergleslab.com

Sounds in silence:  How glial cells in the ear promote development of the auditory system.

Spontaneous electrical activity is a prevalent feature of the developing nervous system, which has been shown to influence the maturation and survival of neurons, as well as the refinement of circuits in the brain.  In the auditory system, bursts of activity are initiated in the cochlea when ATP is released by supporting cells that lie adjacent to inner hair cells (IHCs).  This periodic release of ATP induces inward currents, crenations (cell shrinkage), and Ca2+ waves in supporting cells, events that are associated with periodic depolarization of inner hair cells and subsequent bursts of action potentials in primary auditory neurons.  This activity is prominent during the first two postnatal weeks in mice, prior to hearing onset, suggesting that it may influence development of the cochlea and maturation of central auditory circuits.  In this lecture, I will describe how glial cells in the inner ear have adapted a pathway used for fluid secretion in other organs to induce excitation of hair cells, define several key molecular components of this pathway and show using in vivo imaging how these peripheral glial cells control neural activity in auditory centers of the CNS.

 

host: Ed Rubel

Jan
12
Thu
2017
PBIO Seminar Series: A. Tzingounis @ HSB G-328
Jan 12 @ 9:30 am – 10:30 am

Anastasios V. Tzingounis

University of Connecticut

Seizing the brake: defining the role of KCNQ2/3 channels in the brain

My seminar will focus on KCNQ channels, a potassium channel family implicated in multiple neonatal epileptic encephalopathy disorders. I will first discuss the role of KCNQ2/3 channels in interneurons. Previous research on the function of KCNQ2/3 channels nearly exclusively focused on excitatory neurons, but in fact these channels are also expressed by inhibitory interneurons. Insight regarding the function of KCNQ2/3 channels in interneurons has become critical as some newly identified epilepsy-associated KCNQ2/3 mutations have a gain-of-function effect on channel activity, and such mutations may lead to seizures through diminished inhibitory neuron activity. Second, I will present data regarding the molecular components that underlie the slow afterhyperpolarization (sAHP) in hippocampus and the role of KCNQ2/3 channels in the sAHP.

Host: Andres Barria

Jan
19
Thu
2017
PBIO Seminar Series: Polina Lishko @ HSB G-328
Jan 19 @ 9:30 am – 10:30 am

An ion channel on steroids: the unconventional pathway of calcium regulation by endogenous cannabinoids

Polina Lishko, PhD
Assistant Professor of Cell and Developmental Biology
Department of Molecular and Cell Biology
UC Berkeley

Host: Sharona Gordon

Ion channels control sperm activity by regulating intracellular levels of calcium, which stimulates cell motility and fertility. Steroid hormone progesterone produced by an ovulated egg promotes the entry of calcium through sperm channel CatSper- an event so central for fertilization that men lacking these channels are infertile. We have demonstrated that human CatSper is associated with a membrane progesterone receptor, which makes human spermatozoa controlled by the female reproductive cycle. The identity of this receptor has been recently revealed to be serine hydrolase ABHD2 that degrades endogenous CatSper inhibitor 2-arachidonoylglycerol upon progesterone exposure. ABHD2 is ubiquitously expressed, and the pathway we have discovered in spermatozoa, is likely a universal pathway that defines membrane steroid signaling in other tissues.

Mar
20
Mon
2017
PBIO seminar – Manu Ben-Johny, Ph.D. @ HSB G-328
Mar 20 @ 9:30 am – 10:30 am

Feedback control of sodium and calcium channels – from shared

molecular underpinnings to synthetic modulation.

Manu Ben-Johny, Ph.D.
Department of Bioengineering
Johns Hopkins University

 

Abstract:

The voltage-gated sodium (NaV) and calcium (CaV) channels constitutes two major ion-channel superfamilies with custom molecular attributes that enable a multitude of unique biological functions. For example, the NaV channels support brisk spatial propagation of action potentials while the CaV channels couple excitability to contraction, secretion, and transcription. Yet, one similarity of these channels is their homologous carboxy-tail, a segment that is a hotspot for diverse cardiac arrhythmogenic and neurological disorders. If this region were to support like-functions, deep mechanistic insights could be gleaned from the joint investigation of these channels, and shared principles derived for approaching related channelopathic diseases. For CaV channels, dynamic interactions between their tail domains and calmodulin elaborate rapid and recognizably similar forms of Ca2+-feedback regulation. However, for NaV channels, Ca2+ effects have long-appeared to be subtle and variable with divergent purported mechanisms, dimming prospects for unification. Here, using quantitative Ca2+-photouncaging and single-channel recordings, we show that these differences are only apparent and that Ca2+ regulatory function and mechanism are fundamentally conserved across the two channel families. Despite this high similarity and dependence on a modular structural element, we demonstrate how cells employ distinct auxiliary proteins to selectively switch feedback control of NaV versus CaV channels and vice versa. Overall, these results help substantiate the persistence of an ancient Ca2+-regulatory design across channel superfamilies, unravel the sophisticated mechanisms by which cytosolic proteins tune cellular excitability and calcium signaling, and reveal new strategies to engineer the molecular function of ion channels.

host: Stan Froehner

Mar
23
Thu
2017
PBIO seminar – Yao Chen, Ph.D. @ HSB G-328
Mar 23 @ 9:30 am – 10:30 am

Unexpected lessons on neuromodulator action from imaging molecular signals

Yao Chen, Ph.D.
Department of Neurobiology
Harvard Medical School

 

Abstract:

Neuromodulators such as acetylcholine and dopamine have profound effects on neural circuits and behavior. However, how neuromodulator-induced molecular signals influence behavior represents an outstanding gap in our understanding of neuromodulator action. In order to go beyond the identity to the action of molecular signals, we need to know the subcellular and cellular specificity as well as temporal dynamics of neuromodulator-induced molecular signals. One of the major challenges to uncover these features is the lack of methods to dynamically monitor the molecular signals induced by G protein-coupled receptor (GPCR) activation with high spatial resolution. A critical intracellular integrator of GPCRs is protein kinase A (PKA). PKA activity is stimulated by Gαs-coupled and inhibited by Gαi-coupled neuromodulator receptors, and this push-pull relationship can bidirectionally modulate synaptic function as well as transcription. In order to monitor PKA activity in the brain, we modified a PKA activity sensor and developed optical approaches that enable quantitative analysis of endogenous GPCR signaling in brain tissue with two-photon fluorescence lifetime imaging microscopy. Using this reporter, we have found that, contrary to the canonical model of GPCR signaling, endogenous Gαq-coupled neuromodulator receptors elevate phosphorylation by PKA in the mouse hippocampus. I will present this discovery that highlights PKA as a central integrator of three major types of GPCR signals. Furthermore, I will share ongoing work suggesting that the same neuromodulator input (e.g. acetylcholine) can produce different PKA signals depending on the context of cellular physiology and animal experience. Finally, I will outline my future directions that aim to examine the spatial location, temporal dynamics and context dependence of neuromodulator-induced molecular signals, and how these features of molecular signaling contribute to cellular physiology and animal behavior.

 

 

 

host: Stan Froehner

Mar
30
Thu
2017
PBIO seminar – Arnold Hayer, Ph.D. @ HSB G-328
Mar 30 @ 9:30 am – 10:30 am

Collective endothelial cell migration – cadherin fingers lead the way

Arnold L. Hayer, Ph.D.
Department of Chemical and Systems Biology
Stanford University

Abstract:

The development and maintenance of the vasculature requires collective cell movement, during which neighboring cells coordinate the polarity of their migration machineries. We addressed the unresolved question of how polarity signals are transmitted from one cell to another across symmetrical cadherin junctions, using an in vitro model of collective endothelial cell migration.

We found that collectively migrating endothelial cells have polarized VE-cadherin-rich membrane protrusions, ‘cadherin fingers’, which leading cells extend from their rear and follower cells engulf at their front. In follower cells, engulfment of cadherin fingers occurs along with the formation of a lamellipodia-like zone with low actomyosin contractility, and requires VE-cadherin/catenin complexes and Arp2/3-driven actin polymerization. Lateral accumulation of cadherin fingers in follower cells precedes turning, and increased actomyosin contractility can initiate cadherin finger extension as well as engulfment by a neighboring cell, to promote follower behavior. Cadherin fingers create positively curved membrane surfaces only in the front of follower cells, which selectively recruit and polarize curvature sensing regulatory proteins. Thus, engulfment of cadherin fingers at the cell front converts symmetric cadherin junctions into polarized structures that support collective cell guidance.

Further, I will discuss our recent identification of a BAR domain and RhoGAP protein, which is required both for coordinated endothelial cell movement and vascular sprouting in vitro, and therefore establishes an intriguing mechanistic link between the asymmetric cadherin finger structure and RhoGTPase signaling.

host: Stan Froehner

Apr
6
Thu
2017
2017 Hille Lecture – Thomas Schwarz @ Foege Auditorium
Apr 6 @ 4:00 pm – 5:00 pm

Moving and Removing Axonal Mitochondria

Thomas L. Schwarz, PhD
Professor Neurology
F.M. Kirby Center for Neurobiology
Children’s Hospital, Boston
and Dept. of Neurobiology, Harvard Medical School

Time: 4:00PM

Location: Foege Auditorium, GNOM S060

seminar abstract: Mitochondria are dynamic organelles.  In every cell they move and undergo fission and fusion.  Their distribution and associations with the cytoskeleton change in response to many signals, including the mitotic cell cycle.  In addition, because neurons look like no other cell in the organism, with axons of up to a meter in humans, mitochondrial motility is particularly crucial to the survival of the neuron. The neuron also needs to clear away damaged mitochondria efficiently wherever in the cell they may arise.  Not surprisingly then, defects in the transport machinery of neurons and in their mechanisms for removing damaged mitochondria have been linked to several neurodegenerative diseases, including ALS and Parkinson’s disease.  This talk will present the evidence for a motor/adaptor complex that is responsible for and regulates the movement of mitochondria and will discuss how that movement is regulated by the cell cycle, Ca++, and glucose.  We will look at the operation of two proteins PINK1 and Parkin that are mutated in forms of Parkinson’s disease and examine how these proteins operate in axons to clear away damaged mitochondria that might otherwise compromise the health of the cell.  Particularly in the case of mitophagy, we will consider the special challenges posed for neurons by their extended geometry and the difficulty of having a PINK1-dependent pathway operating far from the soma.