Physiology and Biophysics

Seminars

Oct
12
Thu
2017
PBIO Seminar Series: Liangyi Chen @ HSB G-328
Oct 12 @ 9:30 am – 10:30 am

High spatiotemporal resolution, three-dimension fluorescence imaging of biological samples in vivo

Dr. Liangyi Chen
Professor
Laboratory of Cell Secretion and Metabolism
Institute of Molecular Medicine,
Peking University, Beijing, China

Host: Bertil Hille

Abstract: I will give two stories. (i) One story describes unpublished ultrasensitive Hessian structured illumination microscopy that enables ultrafast and long-term super-resolution (SR) live-cell imaging. At a photon dose one order less than point-scanning microscopy, Hessian-SIM has achieved 88-nm and 188-Hz spatial-temporal resolution for live cells imaging and lasted thousands of images without artifacts. Operating at 1 Hz, Hessian-SIM enables hour-long, time-lapse SR imaging with mitigatable photobleaching, highlighting the possibility of achieving SR imaging with commonly used fluorophores for an unlimited period of time. (ii) The second story is our recent Nature Methods paper, our invention of the fast high-resolution miniature two-photon microscope for brain imaging in freely-behaving mice at the single-spine level. With a headpiece weighing 2.15 g and a new type of hollow-core photonic crystal
fiber to deliver 920-nm femtosecond laser pulses, the mini-microscope is capable of imaging commonly used biosensors at high spatiotemporal resolution (0.64 μm laterally and 3.35 μm axially, 40 Hz at 256 × 256 pixels). It compares favorably with benchtop two-photon microscopy and miniature wide-field fluorescence microscopy in the structural and functional imaging of Thy1-GFP- or GCaMP6f-labeled neurons. Further, we demonstrate its unique application and robustness with hour-long recording of neuronal activities down to the level of spines in mice experiencing vigorous body and head movements or engaging in social interaction.

Oct
26
Thu
2017
PBIO Seminar Series: Brian Kalmbach @ HSB G-328
Oct 26 @ 9:30 am – 10:30 am

“Of Mice and Men: Intrinsic Membrane Properties of Human Cortical Pyramidal Neurons”

Brian Kalmbach, Ph.D.

Allen Institute for Brain Science

host: Nikolai Dembrow

Nov
2
Thu
2017
2017 Lamport Lecture – Gilles Laurent @ T-739 HSB
Nov 2 @ 4:00 pm – 5:00 pm

Evolution and brain computation

I will introduce our work towards identifying principles of brain function and computation, focused on using comparative approaches and exploiting unusual model systems (reptiles, cephalopods) to study sleep, texture perception and cerebral cortex evolution.

Gilles Laurent, PhD, DVM

Director

Max Planck Institute for Brain Research

http://www.brain.mpg.de/home/

 

4:00 PM

Location: T-739, HSB

 

host: Stan Froehner

Dec
7
Thu
2017
PBIO Seminar Series: Nikolai Dembrow @ HSB G-328
Dec 7 @ 9:30 am – 10:30 am

Title: TBA

Nikolai Dembrow, PhD

host: Stan Froehner

Jan
25
Thu
2018
PBIO Seminar Series: Amy Bastian, PhD @ HSB G-328
Jan 25 @ 9:30 am – 10:30 am

 

Amy Bastian, Ph.D.
Professor of Neuroscience
Johns Hopkins University

 

Host: John Tuthill

 

May
10
Thu
2018
2018 Crill Lecture – J. Anthony Movshon @ HSB G-328
May 10 all-day

Time & Location TBA

J. Anthony Movshon, PhD

Professor, Department of Ophthalmology

Professor, Department of Neuroscience and Physiology