Physiology and Biophysics

Seminars

Oct
29
Tue
2019
2019 Lamport Lecture: Brian Kobilka @ Hogness Auditorium
Oct 29 @ 4:00 pm – 5:00 pm

Structural insights into the dynamic process of G protein coupled receptor activation

Brian Kobilka, MD Professor Department of Molecular and Cellular Physiology Stanford University School of Medicine Hélène Irwin Fagan Chair in Cardiology host: Stan Froehner abstract: G protein coupled receptors (GPCRs) conduct the majority of transmembrane responses to hormones and neurotransmitters, and mediate the senses of sight, smell and taste. The b2 adrenergic receptor (b2AR), the M2 muscarinic receptor and the mu-opioid receptor are prototypical Family A GPCRs. We have obtained three-dimensional structures of these receptors in inactive and active conformations, as well as a structure of the b2AR in complex with the G protein Gs. Comparison of these structures provides insights into common mechanisms for propagation of conformational changes from the agonist binding pocket to the G protein coupling interface. Crystal structures of inactive and active states may give the impression that GPCRs behave as simple two-state systems. However, cellular signaling assays reveal that many GPCRs signal through more than one G protein isoform, and through G protein independent pathways. This complex functional behavior provides evidence for the existence of multiple functionally distinct conformational states. We have used fluorescence, EPR and NMR spectroscopy to study the dynamic properties of several GPCRs. I will discuss what we these studies have taught us about allosteric regulation of GPCR structure by G proteins and ligands.    
Dec
5
Thu
2019
PBIO seminar series: Elena Gracheva
Dec 5 @ 9:30 am – 10:30 am

Molecular adaptations to the unique life style in mammalian hibernators.

Elena Gracheva, PhD Associate Professor, Dept Cellular & Molecular Physiology, Dept Neuroscience Yale Abstract: Currently, the vast majority of cellular and molecular research in biological sciences focuses just on a handful of species, and even fewer are used as experimental models. In my lab, we have been developing non-standard animal models. We use hibernating 13-lined Ground squirrels (an obligatory hibernator) and Syrian hamsters (a non-obligatory hibernator), to tackle fundamental biological questions from perspectives unachievable using the standard animal models alone. Specifically, we are interested in studying molecular evolution of mammalian hibernation and cellular adaptations that these animals evolve in order to survive prolonged periods of hypothermia, water deprivation and starvation. We are also trying to pin point the molecular and physiological basis of hibernation induction. Comparative analysis of three rodent species—such as ground squirrels, hamsters and mice (non-hibernator)—at the behavioral, cellular and molecular levels, will help us to delineate the multitude of adaptations that hibernators evolved in order to survive harsh environment, and as a result came to inhabit a wide geographical range.   host: John Tuthill
Dec
12
Thu
2019
PBIO seminar series: Timothy Murphy
Dec 12 @ 9:30 am – 10:30 am
Feb
6
Thu
2020
PBIO seminar series: Bita Moghaddam
Feb 6 @ 9:30 am – 10:30 am
Feb
20
Thu
2020
PBIO seminar series: Zhuan Zhou
Feb 20 @ 9:30 am – 10:30 am

-joint sponsored with Department of Pharmacology-

Voltage-dependent but calcium-independent exocytosis of neurotransmitters

Zhuan Zhou, Ph.D.
Professor and Senior Investigator
Laboratory of Cellular Biophysics and Neurodegeneration
Institute of Molecular Medicine
Peking University, Beijing, P.R. CHINA
host: Bertil Hille
Mar
19
Thu
2020
PBIO seminar series: Michael Reiser
Mar 19 @ 9:30 am – 10:30 am
Michael Reiser HHMI/Janelia   host: John Tuthill
Mar
26
Thu
2020
PBIO seminar series: Bret Smith
Mar 26 @ 9:30 am – 10:30 am
Bret N Smith, PhD Professor and Chair Department of Neuroscience Professor of Physiology Director, Epilepsy Research Center (EpiC) University of Kentucky College of Medicine host: Nick Poolos
Apr
2
Thu
2020
PBio seminar series: Lorrie Kirshenbaum
Apr 2 @ 9:30 am – 10:30 am
Dr. Lorrie A. Kirshenbaum Director, Institute of Cardiovascular Sciences Max Rady College of Medicine, University of Manitoba     Host: Chris Liu
Apr
16
Thu
2020
Barbara Ehrlich 2020 Hille Lecture @ TBD
Apr 16 all-day
  Barbara Ehrlich, PhD
Professor of Pharmacology and of Cellular And Molecular Physiology
Yale School of Medicine
Host: Stan Froehner