Subsecond modulation of cost-benefit decision making by dopamine
R01 MH079292 (NIH/NIMH), PI: Phillips
Phasic dopamine release during decision making. Changes in extracellular dopamine concentration at the initiation of a discrete trial of a concurrent choice, effort-based decision-making task.
Dopamine may play an important role in dealing with these costs. For example, antagonism of dopamine is sufficient to lower the physical effort rats will make, or the amount of time they will wait to obtain greater rewards. Subsecond activity in dopamine neurons encodes the outcome value within a range of available rewards, and these rapid signals can promote decisions to engage in behaviors to obtain rewards. We propose that this fast transmission provides a threshold signal for the costs an animal should endure to obtain a reward. Since they encode the expected reward amplitude, this would drive animals to tolerate a higher cost for better (expected) outcomes.
In order to test this hypothesis, we will use a series of operant decision-making tasks that allow us to assess cost/benefit analysis and normalize across different costs, while measuring dopamine transmission with fast-scan cyclic voltammetry. In addition to investigating the subsecond temporal correlation between dopamine and behavior, we will also test causality of rapid dopamine release in cost/benefit decision making by electrically stimulating dopamine neurons.
First, we will use a single-choice paradigm to test how dopamine in the nucleus accumbens encodes reward value and imposed effort or time costs. Next we will explore this signaling further when animals are choosing between two options that differ in reward magnitudes and in (time or effort) cost. Finally we will evoke dopamine release by electrically stimulating dopamine neurons at the exact time that an endogenous dopamine signal is generated - at the time of the initial stimulus at the start of the trial. This stimulation will boost the endogenous signal and thus test the effect of releasing more dopamine at the critical time during the behavior is sufficient to bias decision making towards higher-cost options.