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SCCPIR Gene Array Facility Personnel
 | Paul Amieux, Ph.D., DirectorPaul Amieux received his Bachelor of Science Degree from San Diego State University in 1990 and spent two years
in the laboratory of Dr. Chris Glembotski studying corticosteroid and catecholamine-dependent regulation of ANF
(Atrial Natriuretic Factor) in primary rat cardiomyocytes. After entering the University of Washington Interdisciplinary
Molecular and Cellular Biology Program in 1991, Paul joined the Department of Pharmacology and the laboratory of
Dr. G Stanley Mcknight where his thesis work involved studying compensatory changes in the PKA enzyme
system which occur upon deletion of various subunits. The final part of his thesis work focused on the
targeted disruption of the RIa regulatory subunit of Protein Kinase A and characterization in vivo
and in cell culture of the early embryonic lethality caused by this mutation. Paul then completed a
postdoctoral fellowship in the Department of Physiology and Biophysics in the laboratory of Mark A. Bothwell
studying the mechanism of regulated cleavage of the low affinity neurotrophin receptor that belongs to the
TNF receptor superfamily. Paul joined the NICHD-funded SCCPIR gene array facility as Co-Director in July 2001.
Paul's main focus in the array facility is to assist SCCPIR researchers with experimental design, developing
statistically rigorous gene array experiments, and staying abreast of the latest developments in gene clustering
and statistical analysis of microarray data. |
 | G. Stanley McKnight, Ph.D., Co-DirectorDr. G. Stanley McKnight, Co-Director of the SCCPIR Gene Array Facility, has had a long term interest in molecular biology
and its application to endocrinology. As a graduate student of Dr. Robert T Schimke at Stanford University, Dr. McKnight
worked extensively in the chick oviduct model of steroid hormone regulation of gene expression. After postdoctoral
fellowships in Strasbourg working with Dr. Pierre Chambon and at the University of Washington working with
Dr. Richard D. Palmiter, Stan joined the Department of Pharmacology at the University of Washington in 1979 where his
lab first successfully isolated and cloned many of the cDNAs for the regulatory and catalytic subunits of the
cAMP-dependent Protein Kinase (PKA). Dr. McKnight's laboratory went on to develop expression vectors for the various
regulatory and catalytic subunits of PKA and subsequently performed gene knockouts for each of the PKA subunits followed
by detailed and ongoing analyses of the phenotypes of these knockouts which have led the lab into a variety of fields,
including neurobiology, development, metabolism /body weight regulation, and male reproduction. The arrival of microarray
technology in the mid-1990s and its extensive use for analysis of global changes in gene expression in various paradigms
was an obvious choice as an addition to our laboratory' s existing analysis systems. We are currently looking at changes
in gene expression patterns in testis and other tissues involved in reproduction comparing wild type and PKA mutant mice. |
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