charles w. frevert, dvm, scd
Department of Comparative Medicine
Division of Pulmonary and Critical Care Medicine
Education and Training
D.V.M., Iowa State University, Ames, IA, 1984.
Sc.D., Harvard University, Cambridge, MA, 1994.
Internship in Large Animal Internal Medicine, Tufts University, Medford, MA, 1985.
Residency in Large Animal Internal Medicine, Tufts University, Medford, MA, 1987.
Clinical fellowship in Large Animal Internal Medicine, Tufts University and Harvard University, Cambridge, MA, 1988.
Fellowship in Respiratory Biology Program, Harvard University, Cambridge, MA, 1989.
Fellowship in Physiology Program completed at Harvard University, Cambridge, MA, 1995.
Our laboratory investigates the cellular and molecular mechanisms that regulate inflammatory responses in the lungs. The research in my laboratory focuses on the interaction of chemokines with extracellular matrix and the regulation of cytokine and chemokine production in the lungs. We use a variety of different methods, including techniques to study whole animals, techniques to study intact leukocytes and other cells in vitro, and molecular techniques to study morphological changes in tissue.
A major goal of this laboratory is to investigate the basic mechanisms that regulate the activity of chemokines (chemotactic cytokines) in normal and inflamed lungs. Several lines of evidence from in vitro studies suggest that chemokines bind to glycosaminoglycans and that this binding modulates the interactions between chemokines and their cognate receptors (e.g., CXCR1). There is also strong evidence that specific binding domains are located on chemokines and on glycosaminoglycans. This suggests that the interaction of chemokines to glycosaminoglycans is a specific interaction mediated by a subset of proteoglycans in the lungs (e.g., chemokine-binding proteoglycans). Currently the laboratory is focusing on four areas of chemokine-proteoglycan interactions.
Frevert CW, Huang SL, Danaee H, Paulauskis JD, Kobzik L. Functional characterization of the rat chemokine KC and its importance in neutrophil recruitment in a rat model of pulmonary inflammation. J Immunol 1995;154:335-344
Frevert CW, Weller E, Warner AE, Brain JD. The effect of endotoxin on in vivo rat alveolar macrophage phagocytosis. Experimental Lung Research 1998;24:745-758.
Frevert CW, Wong VA, Goodman RB, Goodwin R, Martin TR. Rapid fluorescence-based measurement of neutrophil migration in vitro. J Immunol Methods 1998;213:41-52.
Frevert CW, Matute-Bello G, Skerrett SJ, Goodman RB, Kajikawa O, Sittipunt T, Martin TR. Effect of CD14 Blockade in rabbits with Escherichia coli pneumonia and sepsis. J of Immunol 2000;164:5439-5445.
Matute-Bello G, Frevert CW, Liles WC, Nakamura M, Ruzinski JT, Ballman K, Wong VA, Vathanaprida C, Martin TR. The Fas/FasL system mediates epithelial injury, but not pulmonary host defenses, in response to inhaled bacteria. Infect and Immun 2001;69:5768-5776
Frevert CW, Goodman RB, Kinsella MG, Kajikawa O, Ballman K, Clark-Lewis I, Proudfoot AEI, Wells TNC, Martin TR. Tissue-specific mechanisms control the retention of interleukin-8 in lungs and skin. J Immunol 2002; 168(7):3550-3556.