pradeep singh, MD
Professor, Medicine and Microbiology
Division of Pulmonary and Critical Care Medicine
University of Washington Medical Center
1959 N.E. Pacific, Campus Box 357242
Seattle, WA 98195
Academic Office: 206-221-7151
Fax: (206) 616-2938
Education and Training
BS in Biology (Concentration in Biophysics), Columbia University, NY, NY 1985.
MD, Northwestern University Medical School, Evanston, IL, 1989.
Residency, Department of Internal Medicine, University of Iowa Hospitals and Clinics, Iowa City, IA, 1989-92.
Fellowship, Pulmonary Diseases, Critical Care, and Occupational Medicine, University of Iowa Hospitals and Clinics,Iowa City, IA, 1995-99.
My research focuses on the biofilm mode of bacterial growth. In many chronic infections bacteria live in biofilms rather than as free-living individual organisms. Biofilms are communities of bacteria, living associated with a surface, encased in a polymeric matrix. Biofilm infections include bacterial endocarditis; osteomyelitis; certain skin, urinary and biliary tract infections; dental caries; and most medical device infections. A prototypical example of a biofilm disease is the chronic P. aeruginosa airway infections that afflict patients with cystic fibrosis and other forms of bronchiectasis. Like other biofilm diseases, these infections are extremely difficult to eradicate.
Currently our work focuses on the following areas: We have found that short-term biofilm growth generates extensive genetic diversity in biofilm bacteria, and this process increases the ability of the biofilm community to withstand physiological stress. We are investigating the mechanisms that produce this diversity. We are also studying mechanisms through which bacteria leave established biofilms, and return to the free living state. Interestingly, biofilm bacteria themselves possess active mechanisms to separate from biofilms, and it is possible that these mechanisms could be exploited in therapeutic approaches. We are also investigating other agents that have the potential to inhibit biofilm formation.
Singh PK, Schaefer AL, Parsek MR, Moninger TO, Welsh MJ, Greenberg EP. Quorum-Sensing Signals Indicate that Cystic Fibrosis Lungs are Infected with Bacterial Biofilms. Nature, 407(6805):762-764, 2000.
Singh PK, Parsek MR, Greenberg EP, Welsh MJ. A Component of Innate Immunity Prevents Bacterial Biofilm Development. Nature, 417:552-555, 2002.
Parsek MR, Singh PK. Bacterial Biofilms: An Emerging Link to Disease Pathogenesis. Ann. Rev. Microbiol., 57:677-701, 2003.
Boles BR, Thoendel M, Singh PK. Self-Generated Diversity Produces "Insurance Effects" in Biofilm Communities. Proc. Natl. Acad. Sci. USA, 101(47):16630-5, 2004.
Boles BR, Thoendel M, Singh PK. Rhamnolipids Mediate Detachment of Pseudomonas aeruginosa from Biofilms. Mol. Microbiol., 57(5):1210-23, 2005.