Mark M. Wurfel, MD, phd
Division of Pulmonary and Critical Care Medicine
Harborview Medical Center
325 Nnth Avenue, Box 359640
Seattle, WA 98104
Academic Office: 206-897-5387
Fax: (206) 897-5392
Education and Training
BA in Economics, University of Michigan, Ann Arbor, MI 1989.
Ph.D. in Immunology, The Rockefeller University, New York, NY 1995.
Research Fellow, Rockefeller University, New York, NY 1996.
MD, Cornell University Medical College, New York, NY 1997.
Internship, University of Washington, Seattle WA 1998.
Residency, Traditional Track, University of Washington, Seattle WA 2000.
Chief Residency, Swedish Medical Center, SeattleWA 2000.
Basic and translational research on host susceptibility to sepsis and acute lung injury,
with a focus on genetic factors. We have found that there exists a high level of inter-individual variability in the magnitude of inflammation generated by healthy human leukocytes after exposure to bacterial products. We use a simple ex vivo assay to screen innate immune inflammatory responses in healthy volunteers and then identify genetic variants (polymorphisms) that control this variability using high throughput genotyping techniques. We then determine whether genetic variants found to cause very high or very low responses in the healthy population predict bacteremia, lung injury, and other organ dysfunction in critically ill patients with sepsis. In addition, we use a combination of in vitro techniques such as cloning, site-directed mutagenesis, siRNA knockdown, and surface and intracellular flow cytometry to understand the molecular and cellular alterations caused by these genetic variants.
Wurfel MM, Mons BG, Ingalls RR, Dedrick RL, Delude R, Zhou D, Lamping N, Schumann RR, Thieringer R, Fenton MJ, Wright SD, Golenbock D. Targeted deletion of the lipopolysaccharide (LPS)-binding protein gene leads to profound suppression of LPS responses ex vivo, whereas in vivo responses remain intact. J Exp Med 1997;186:2051-6.
Wurfel MM, Park WY, Radella F, Ruzinski J, Sandstrom A, Strout J, Bumgarner RE, Martin TR. Identification of high and low responders to lipopolysaccharide in normal subjects: an unbiased approach to identify modulators of innate immunity. J. Immunol. 2005;175:2570-2578.
Hallstrand TS, Fischer ME, Wurfel MM, Afari N, Buchwald D, Goldberg J. Genetic pleiotropy between asthma and obesity in a community-based sample of twins. J Allergy Clin Immunol 2005;116(6):1235-41.
Lee JS, Wurfel MM, Matute-Bello G, Frevert CW, Rosengart MR, Ranganathan M, Wong VW, Holden T, Sutlief S, Richmond A, Peiper S, Martin TR. The Duffy antigen modifies systemic and local tissue chemokine responses following LPS stimulation. J Immunol 2006 Dec 1;177(11):8086-94.
Wurfel MM. Microarray-based analysis of ventilator-induced lung injury. Proc Am Thorac Soc 2007 Jan;4(1):77-84.
Storey JD, Madeoy J, Strout JL, Wurfel MM, Ronald J, Akey JM. Gene-expression variation within and among human populations. Am J Hum Genet. 2007 Mar;80(3):502-9.