RESEARCH REAGENTS

 

Human islet glutamic acid decarboxylase (GAD65) cDNA

 

Our laboratory cloned the human islet GAD65 cDNA (Karlsen et al Cloning and primary structure of human islet isoform of glutamic acid decarboxylase from chromosome 10. PNAS 88:8337-8341, 1991.) Originally the GAD65 cDNA was available to researchers as a modified pcDNAII vector called pEX9 (Grubin et al A novel radioligand binding assay to determine diagnostic accuracy of isoform-specific glutamic acid decarboxylase antibodies in childhood IDDM. Diabetologia 37:344-350, 1994.)  Recently we have subcloned the human GAD65 cDNA into pTnT^TM Vector (Promega PN L5610) that improves the translation performance and produces a protein that runs as a single band on an SDS polyacrylamide gel.