Rubel 2048-02 Chemical agents/drugs administered to animals

3/2/06

1. Regarding your work with the agents noted below. If these agents are in powder form they must be weighed, reconstituted, and diluted in a chem fume hood or a ducted biosafety cabinet wearing compatible chem resistant gloves and other appropriate Personal Protective Equipment (PPE) including safety eyewear.

If these agents are in vial form requiring reconstitution, they must be reconstituted in a chem fume hood or a ducted biosafety cabinet wearing compatible chem resistant gloves and other appropriate PPE including safety eyewear.

As these agents are either: (a) have a Health Hazard rating of 3, (b) a reproductive hazard, (c) are noted as toxic, (d) may cause harm to an unborn child, (e) are sensitizers or may cause sensitization through the inhalation or dermal route, or (f) may cause heritable genetic effects, personnel working with any of these agents must read their Material Safety Data Sheet (MSDS) to address the requirement for hazard awareness training.

ALK-5 inhibitor

BrdU

Ethacrynic acid

Furosemide

Gentamicin

Inhibitors of mitochondrial membrane potential: bongkrekic acid, cyclosporin A, and rotenone

Kanamycin

Lead salts: lead acetate

Nembutal

Neomycin

Non- Steroidal Anti-inflammatory agents: Indomethacin, Sulindac, Sulindac sulfate, Sulindac sulfone)

Streptomycin

Urethane

Oxymorphone

Ketamine

Atropine

Pentobarbital

2. Regarding your work with Cisplatin, I have attached EH&S’ SOP for Anti-neoplastic agent use in the lab, for your implementation.

3. Regarding your work with the agents listed below. You must contact Katia Harb, Industrial Hygienist, at 616-3771 to develop or review your SOPs for work with these agents. If these agents are in powder form they must be weighed, reconstituted, and diluted in a chem fume hood or a ducted biosafety cabinet wearing compatible chem resistant gloves and other appropriate Personal Protective Equipment (PPE) including safety eyewear.

If these agents are in vial form requiring reconstitution, they must be reconstituted in a chem fume hood or a ducted biosafety cabinet wearing compatible chem resistant gloves and other appropriate PPE including safety eyewear.

As these agents have a Health Hazard rating of 4, personnel working with either of these agents must read their Material Safety Data Sheet (MSDS) to address the requirement for hazard awareness training.

CN (cyanide)

Tetrodotoxin

4. Regarding your work with the agents noted below. Radionuclide work per Radiation Safety Office.

14 C-2-deoxyglucose

³H Thymidine

5. Regarding your work with the agents noted below. Please review their MSDS for recommendations for exposure control and health hazards. Exposure control methods are found in Section 8 of the MSDS. Please follow their recommendations. Health hazard information is found in Section 3. If any of these agents have: (a) have a Health Hazard rating of 3, (b) a reproductive hazard, (c) are noted as toxic, (d) may cause harm to an unborn child, (e) are sensitizers or may cause sensitization through the inhalation or dermal route, or (f) may cause heritable genetic effects, personnel working with any of these agents must read their Material Safety Data Sheet (MSDS) to address the requirement for hazard awareness training.

GSK-3 inhibitors

Metafane

MitoFox Green

Succinimidyl esters of Texas Red conjugated neomycin and gentamicin

All labels noted in the zebra fish section under #4. Administration of drugs/reagents/cells.