We are interested in developing simple genetic strategies in yeast to analyze protein functions.
These strategies include assays that analyze proteins, DNA, RNA, and small molecules.
 RNA Technology:
We are identifying transcription factor binding sites with a DNaseI footprinting assay, investigating the role of DrnI in intron turnover, and selecting for RNAs that contain 3' cyclic phosphate moeities.
DNA Technology:
We are analyzing chromosome conformation in yeast.
Protein Technology:
We are developing a random-addressed protein array format, developing a malaria protein array, and identifying protein targets of ubiquitin ligases.
Yeast Metabolism:
We are using capillary electrophoresis & mass spectrometry to analyze the yeast metabolome.
Yeast Protein Interactions:
We are developing a dual-reporter 2-hybrid assay to eliminate technical false positives. Also, we have been carrying out two-hybrid analysis on yeast proteins using a protein array format and collaborated with CuraGen Corporation in a complementary genomic analysis of yeast interactions. Please see the NCRR Yeast Resource Center page in order to work with us on a screen for your favorite yeast protein.
Previous Projects:
In previous work, we have carried out a genomewide indentification of spliced introns, attempted to identify proteins that function in recombination, studied yeast aging using replicative assays and chronological assays, analyzed WW domain interactions using yeast protein arrays, identified interactions of yeast membrane proteins, used the two-hybrid array to analyze interactions of spindle-associated proteins, used the tethered catalysis two-hybrid system to study chromatin modification, participated in a collaborative effort to characterize unknown yeast ORFs, carried out a yeast screen to identify protein-protein interactions of the malaria parasite P. falciparum and mefloquine resistance genes, used the two-hybrid assay to screen for interactions of Toll-like receptors, devised a new screening strategy involving the use of leucine zippers to identify proteins that function in a common process, carried out chemical profiling of the yeast deletion collection, developed a biosensor that reports the binding of small molecules, and applied a genomewide screen for DNA-binding activities.
NCRR Yeast Resource Center:
Our lab is part of a Resource Center that provides expertise and access to two-hybrid assays, mass spectrometry, deconvolution fluorescence microscopy and protein prediction. The mission of the Center is to facilitate the identification and characterization of protein complexes in the yeast Saccharomyces cerevisiae.
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