Publications describing yeast protein interactions that include work from the laboratory are listed here:

Montpetit B, Hazbun TR, Fields S, Hieter P. Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation. J Cell Biol. 2006 Aug 28;174(5):653-63. download pdf


Jin F, Hazbun T, Michaud GA, Salcius M, Predki PF, Fields S, Huang J. A pooling-deconvolution strategy for biological network elucidation. Nature Methods. 2006 Mar;3(3):183-9. download pdf

Fields S. (2005) High-throughput two-hybrid analysis. The promise and the peril. FEBS J. Nov;272(21):5391-9. download pdf

Antoniacci LM, Kenna MA, Uetz P, Fields S, Skibbens RV. (2004) The spindle pole body assembly component mps3p/nep98p functions in sister chromatid cohesion. J Biol Chem. Nov 19;279(47):49542-50. download pdf

Guo, D., Hazbun, T.R., Xu, X.-J., Ng, S.-L., Fields, S. and Kuo, M.-H. (2004) A tethered catalysis two-hybrid system to identify protein-protein interactions requiring post-translational modifications. Nature Biotechnology Jul;22(7):888-892. download pdf

Hazbun, T.R., Malmström, L., Anderson, S., Graczyk, B.J., Fox, B., Riffle, M., Sundin, B.A., Aranda, J.D., McDonald, W.H., Chun, C., Snydsman, B.E., Bradley, P., Muller, E.G.D., Fields, S., Baker, D., Yates, J.R. III and Davis, T.N. (2003) Assigning function to yeast proteins by integration of technologies. Molecular Cell 12:1353-1365. download pdf

Guo, M., Uetz, P., Burchett, S.A., Fields, S. and Dohlman, H.G. (2003) The yeast G protein alpha subunit Gpa1 transmits a signal through a novel effector RNA-binding protein Scp160. Mol. Cell, 12: 517-524. download pdf

Shang, C., Hazbun, T.R., Cheeseman, I.M., Aranda, J., Fields, S., Drubin, D.G. and Barnes, G. (2003) Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p. Molecular Biology of the Cell14(8):3342-55. download pdf

Kitagawa, K., Abdulle, R., Bansal, P.K., Cagney, G., Fields, S. and Hieter, P. (2003) Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint. Molecular Cell 11:1201-1213. download pdf

Phizicky, E., Bastiaens, P. I. H., Zhu, H., Snyder, M. and Fields, S. (2003) Protein analysis on a proteomic scale. Nature 422:208-215. download pdf

Pot, I., Measday, V., Snydsman, B., Cagney, G., Fields, S., Muller, E., Davis, T.N. and Hieter, P. (2002) Chl4p and Iml3p are two new members of the budding yeast outer kinetochore. Mol. Biol. Cell 14(2):460-76. download pdf

Stagljar, I. and Fields, S. (2002) Analysis of membrane protein interactions using yeast-based technologies. Trends in Biochemical Sciences 27: 559-563. download pdf

von Mering, C., Krause, R., Snel, B., Cornell, M., Oliver, S.G., Fields, S. and Bork, P. (2002) Comparative assessment of large-scale data sets of protein-protein interactions. Nature 417:399-403. download pdf

Burchett, S.A., Flanary, P., Aston, C.W., Jiang, L., Young, K.H., Uetz, P., Fields, S. and Dohlman, H.G. (2002) Regulation of stress response signaling by the N-terminal DEP (dishevelled/EGL-10/pleckstrin) domain of Sst2, a regulator of G protein signaling in Saccharomyces cerevisiae. J. Biol. Chem. 277:22156-67. download pdf

Measday, V., Hailey D., Pot, I., Givan, S., Hyland, K., Cagney, G., Fields, S., Davis, T. and Hieter, P. (2002) The Mis6 budding yeast homologue, Ctf3p, is an outer kinetochore protein that interacts with Mcm22p and Mcm16p but is not required for Cse4p/CENP-A to bind centromere DNA . Genes and Development 16:101-113. download pdf

Gera, J.F., Hazbun, T.R. and Fields, S. (2002) Array-based methods for identifying protein-protein and protein-nucleic acid interactions. Methods Enzymol. 350:499-512.

Tong, A. H., Drees, B., Nardelli, G., Bader, G. D., Brannetti, B., Castagnoli, L., Evangelista, M., Ferracuti, S., Nelson, B., Paoluzi, S., Quondam, M., Zucconi, A., Hogue, C. W., Fields, S., Boone, C., and Cesareni, G. (2001) A combined experimental and computational strategy to define protein interaction networks for peptide recognition modules. Science 295:284-7. download pdf

Cagney, G., Uetz, P. and Fields, S. (2001) Two-hybrid analysis of the Saccharomyces cerevisiae 26S proteasome. Physiol. Genomics 7:27-34. download pdf

Drees,B.L., Sundin, B., Brazeau, E., Caviston, J.P., Chen, G.-C., Guo, W., Kozminski, K.G., Lau, M.W., Moskow, J.J., Tong, A., Schenkman, L.R., McKenzie, A. III, Brennwald, P., Longtine, M., Bi, E., Chan, C., Novick, P., Boone, C., Pringle, J.R., Davis, T.N., Fields, S. and Drubin, D.G. (2001) A protein interaction map for cell polarity development. J. Cell Biol. 154: 549-571. download pdf

Hazbun, T.R. and Fields, S. (2001) Networking proteins in yeast. Proc. Natl. Acad. Sci. U.S.A. 98: 4277-4278. download pdf

Cagney, G., Uetz, P. and Fields, S. (2000) High-throughput screening for protein-protein interactions using two-hybrid assay. Meth. Enzym. 328: 3-14.

Schwikowski, B., Uetz, P. and Fields, S. (2000) A network of protein-protein interactions in yeast. Nature Biotech. 18: 1257-1261. download pdf

HHMI,
Department of

Genome Sciences
& Medicine,
Univ. of Washington

USPS Mail:
Univ. of Washington
Box 355065
Seattle, WA 98195
Phone: (206)616-4523
Fax: (206)685-9720


Parcel Mailing:
1705 NE Pacific St
Foege Building,
S313
Seattle, WA 98195