Hydrogel microbead based immunoassay
The droplet microfluidics and external gelation setup is used to fabricate alginate microspheres immobilized with antibodies. The specific binding events of functionalized alginate spheres show specificity comparable to that of the ELISA assay. Our alginate microsphere approach can be utilized for specificity tests, disease diagnosis, targeted drug delivery and drug discovery.
- W. Chen, J.H. Kim, D. Zhang, K.H. Lee, G. A. Cangelosi, S. D. Soelberg, C.E. Furlong, J.-H. Chung, A. Q. Shen, Microfluidic one-step synthesis of alginate microspheres immobilized with antibodies, Journal of the Royal Society Interface, 2013, 10, 20130566.