Laboratory Methods in Synthetic Biology
- Offered as BIOE 425C, CSE 488C, EE 425C
- Winter 2012
- Instructor: Eric Klavins
- Teaching Assistant: Robert Egbert
- Schedule: The first meetings of the quarter will be 1/3 or 1/4 from 3:30 - 5:00 -- you can attend either one. The second meetings will be 1/10 or 1/11 from 3:30-5:00. Starting 1/23 we will also meet with everyone on Mondays from 3:30 - 4:20 in 031 (note that we do *not* meet 1/9 or 1/16). You will also need to meet with your team and schedule your own lab work, which can be any time that works for you.
- Meeting Room: EE 031 (basement)
- Lab Room: EE B031 (sub-basement)
In this course we design and build synthetic gene regulatory circuits for bacteria that can perform simple functions. This quarter we will focus on two applications: diagnostics and biofuels. Students will learn gene regulatory network design, specification, gene synthesis, cloning, experiment design, microscopy, cytometry, and other fundamental methods in synthetic biology. The course is organized around projects, with small teams, weekly meetings to present progress, and open scheduling for lab work. Pre-requisite: Introduction to Synthetic Biology or a similar course, or permission from the instructor.