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User:Klavins - SOSwiki

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(BioCircuits Log)
(BioCircuits Log)
* '''1/26''': We took the plates out of the incubator and put them in the refrigerator that we inherited with a bunch of possibly abandoned stuff in it. The frig doesn't smell very good. We will need to figure out how to dispose of everything and then clean the frig really well.  Josh and I also perused the biobricks wiki and dreamed of the possibilities. It looks like there will be more equipment that we will need if we want to do anything beyond what is in our textbook. Sigh. In for a penny in for a pound, I suppose.
* '''1/26''': We took the plates out of the incubator and put them in the refrigerator that we inherited with a bunch of possibly abandoned stuff in it. The frig doesn't smell very good. We will need to figure out how to dispose of everything and then clean the frig really well.  Josh and I also perused the biobricks wiki and dreamed of the possibilities. It looks like there will be more equipment that we will need if we want to do anything beyond what is in our textbook. Sigh. In for a penny in for a pound, I suppose.
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* '''2/1''': We transfer plated e. coli to LB suspensions today. Our shaker incubator has not arrived yet, so we had to "invent" something: a vortexer with a styrofoam tub holder taped to the top -- all stuck into an incubator. [[image:Homemade-shaker-incubator.jpg|thumb|right|140px|Homemade Shaker-Incubator]] It is a little too vigourous. I hope it holds up for the next 20 hours. This is definitely not a long term solution! We also visited the chem-shop for supplies: test-tube racks, foil and pens. The new water-bath and another set of pippetors showed up too.
+
[[image:Homemade-shaker-incubator.jpg|thumb|right|140px|Homemade Shaker-Incubator]]
 +
 
 +
* '''2/1''': We transfer plated e. coli to LB suspensions today. Our shaker incubator has not arrived yet, so we had to "invent" something: a vortexer with a styrofoam tub holder taped to the top -- all stuck into an incubator. It is a little too vigourous. I hope it holds up for the next 20 hours. This is definitely not a long term solution! We also visited the chem-shop for supplies: test-tube racks, foil and pens. The new water-bath and another set of pippetors showed up too.
* '''2/2''': Well, the shaker sort of fell apart sometime in the night, but things seem fine anyway. Nothing spilled. Today we made a new suspension and measured the OD every 20 minutes to see the growth curve. This worked great!  
* '''2/2''': Well, the shaker sort of fell apart sometime in the night, but things seem fine anyway. Nothing spilled. Today we made a new suspension and measured the OD every 20 minutes to see the growth curve. This worked great!  

Revision as of 17:43, 6 February 2007