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User:Klavins - SOSwiki

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(BioCircuits Log)
(BioCircuits Log)
:[[image:Biocircuits-lab2-sampledata.jpg|thumb|right|140px|Our first growth curve. The 2nd to the last data point might be an error.]] We also looked at the bugs in the microscope and made a nice movie of them swimming around. They all seemed quite please with the LB. We grew the cultures in 500ml flasks, which we had to clean. Our autoclave sterilizes, but makes things dirty somehow on the outside of the flasks. I think the basket is rusty.
:[[image:Biocircuits-lab2-sampledata.jpg|thumb|right|140px|Our first growth curve. The 2nd to the last data point might be an error.]] We also looked at the bugs in the microscope and made a nice movie of them swimming around. They all seemed quite please with the LB. We grew the cultures in 500ml flasks, which we had to clean. Our autoclave sterilizes, but makes things dirty somehow on the outside of the flasks. I think the basket is rusty.
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* '''2/8''': Restriction analysis.
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* '''2/8, 2/15''': We tried the restriction analysis twice. The first time, we all got terrible gels. The second time, it got a bit better, mostly due to the fact that we had all the proper reagents. But we still need to produce gels reliably, so I think we will practice some more. In the mean time, we got a new shaker-incubator -- it is beginning to look like a real lab!
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* '''2/22-2/23''': I was out of town, but Josh and Ranjana did the e coli transformation. It seemed to work, but not fabulously. I think we need to use freshly plated e coli -- so we'll try it again.

Revision as of 19:35, 26 February 2007