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MICROARRAY DATA

PROCEDURES

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PICTURES

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LAB MEETINGS

SEATTLE FLY CLUB

LAB SUPPLIES & RESOURCES

• Antibody staining

• Dacapo staining

• Human ES culture techniques

• Human ES cell media

• Embryo staining

• Competent cells for Electroporation

• Chemical transformation

• Chemically competent E.coli cells

• DEAE Extraction of DNA from agarose gels

•  Fruit Juice Agar/Egg-laying plates recipe

• Ovary RNA/DNA in situ protocol

• Making Dig labeled DNA/RNA probes

• Amino-ally Reverse Transcription Labeling

• LacZ staining protocol

• MIDI PREP by alkaline lysis/Qiagen-tip 100 isolation

• Nuclear isolation from Ovaries and FACS Collection of Nuclei

• Preparation of Follicle cells from whole ovaries for FACS analysis or RNA extraction

• Guanidinium-Hot Acidic Phenol RNA isolation

• Sequencing protocol (Big Dye Ter-minator)

• Tris-Tricine Protein/Peptide Sepa-ration Gels

• Western Total Lysate Preparation Protocol

• WW protein expression, purification and polarization assay

• Zhan's cell lysis for westerns-protocol

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