Publications:
Publications:
Qi J, Yu JY, Shcherbata HR, Mathieu J, Wang AJ, Seal S, Zhou W, Stadler BM, Bourgin D, Wang L, Nelson A, Ware C, Raymond C, Lim LP, Magnus J, Ivanovska I, Diaz R, Ball A, Cleary MA, Ruohola-Baker H., microRNAs Regulate Human Embryonic Stem Cell Division., Cell Cycle. 2009 Nov 15;8(22):3729-41. Epub 2009 Nov 10.
Reynolds SH, Ruohola-Baker H., PIWI goes solo in the soma. , Dev Cell. 2009 May;16(5):627-8.
Yu JY, Reynolds SH, Hatfield SD, Shcherbata HR, Fischer KA, Ward EJ, Long D, Ding Y, Ruohola-Baker H. (2009)Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells., Development. 2009 May;136(9):1497-507. Epub 2009 Mar 31.
Yatsenko AS, Kucherenko MM, Pantoja M, Fischer KA, Madeoy J, Deng WM, Schneider M, Baumgartner S, Akey J, Shcherbata HR, Ruohola-Baker H. (2009) The conserved WW-domain binding sites in Dystroglycan C-terminus are essential but partially redundant for Dystroglycan function, BMC Dev Biol. 2009 Feb 27;9:18.
Bar, M., Wyman, S.K., Fritz, B.R.,
Qi, J., Garg, K.S., Parkin, R., Kroh,
E.M., Bendoraite, A.A., Mitchell, P.S., Nelson, A., Ruzzo, W.L., Ware,
C., Radich, J., Gentleman, R., Ruohola-Baker, H., Tewari, M. (2008)
MicroRNA discovery and profiling in human embryonic stem cells by deep
sequencing of small RNA libraries. Stem Cells,
(10):2496-505 [Epub
Jun 26]
Stadler, B.M and Ruohola-Baker,
H. (2008)
Small RNAs: Keeping Stem
Cells in Line. Cell, 132:563-566.
Reynolds, S. and Ruohola-Baker,
H. (2008) microRNA’s Role in Germline
Differentiation. July 31, StemBook, ed. The Stem cell Research Community,
StemBook, doi/10.3824/stembook.1.17.1,
http://www.stembook.org
Kucherenko MM, Pantoja M, Yatsenko AS, Shcherbata HR, Fischer KA, Maksymiv DV, Chernyk YI, Ruohola-Baker H. (2008) Genetic Modifier screens reveal new Drosophila DG-Dys complex interactors. PlosOne, 3(6):e2418
Hatfield, S. and Ruohola-Baker,
H. (2008)
microRNA and
stem cell
function. Cell Tissue Res., 331:57-66
Shcherbata, H.R., Ward, E.J.,
Fischer, K.A., Yu, J-Y., Reynolds, S.H.,
Chen, C-H., Xu, P., Hay, B.A. and Ruohola-Baker, H. (2007)
Stage-Specific Differences in the Requirements for Germline Stem Cell
Maintenance in the Drosophila Ovary. Cell Stem Cell,
1:685-698
Yatsenko, A.S., Gray, E.E., Shcherbata, H.R., Patterson, L.B., Sood, V., Baker, D., and Ruohola-Baker, H. (2007) A putative SH3-domain binding motif but not the C-terminal Dystrophin WW-domain binding motif is required for Dystroglycan function in cellular polarity in Drosophila, J Biol. Chem. 282:15159-69.
Shcherbata, H.R., Yatsenko, A.S., Patterson, L.B., Sood, V.D., Nudel, U., Yaffe, D., Baker, D., and Ruohola-Baker, H. (2007) Dissecting Muscle and Neuronal Disorders in Drosophila model for Muscular Dystrophy. EMBO J. 26:481-93.
Ward, E.J., Shcherbata, H.R., Reynolds, S., Fischer, K.A., Hatfield, S., and Ruohola-Baker, H. (2006) Stem Cells Signal to the Niche through the Notch pathway in the Drosophila ovary. Curr. Biol. 16:2352-2358.
Ward, E.J., Zhou, X., Riddiford, L.M., Berg, C.A., Ruohola-Baker, H. (2006) Border of Notch activity establishes a boundary between the two dorsal appendage tube cell types. Dev.Biol. 297:461-470.
Hatfield, S., Shcherbata, H.R., Ward, E.J., Reynolds, S., Fischer, K.A., and Ruohola-Baker, H. (2006) MicroRNAs and their involvement in Stem Cell division, in microRNAs; Biology, Function and Expression, ed. Clarke N. and Sanseau, P., DNA Press
Shcherbata, H.R., Hatfield, S., Ward, E.J., Reynolds, S., Fischer, K.A., and Ruohola-Baker, H. (2006) The MicroRNA pathway plays a regulatory role in stem cell division. Cell Cycle 5:172-5.
Jordan, K.C., Schaeffer, V., Fischer, K.A., Gray, E.E., and Ruohola-Baker, H. (2006) Notch signaling through Tramtrack bypasses the mitosis promoting activity of the JNK pathway in the mitotic-to-endocycle transition of Drosophila follicle cells, BMC Dev. Biol. 6:16
Hatfield SD, Shcherbata HR, Fischer KA, Nakahara K, Carthew RW, Ruohola-Baker H. (2005) Stem cell division is regulated by the microRNA pathway. Nature. 435:974-8.
Althauser, C., Jordan, K., Deng, W-M., and Ruohola-Baker, H (2005) Fringe dependent Notch activation through Tramtrack is required for specification of the polar cells in Drosophila oogenesis, DevDyn Drosophila Special Issue, 232:1013-1020.
Jordan,
K., Hatfield, S., Tworoger, M., Ward, E.J., Fischer, A, Bowers, S., and
Ruohola-Baker, H. (2005)
Genome wide analysis of transcript levels after perturbation of the EGFR pathway
in the Drosophila ovary, Dev Dyn. 232:709-724.
Shcherbata, H.R., Althauser, C., Schaeffer,V., Findley, S.D., and Ruohola-Baker, H. (2004) The mitotic-to-endocycle switch in Drosophila follicle cells is executed by Notch dependent regulation of G1/S-, G2/M- and M/G1- cell cycle transitions, Development, 131(13):3169-81
Off with mitosis, on with endocycling
Usually, each step in the cell cycle depends on the completion
of the previous stage. But some cells – for example, cardiomyocytes,
Drosophila egg follicle cells and many cancer cells – can
endocycle, where DNA synthesis continues without mitosis. On
p. 3169, Shcherbata and
colleagues investigate how Notch signalling controls the mitotic-to-endocytic
transition in Drosophila follicle cells at mid-oogenesis. By
identifying genes whose transcription is responsive to Notch at
this transition, the researchers show that Notch activity: (1)
blocks the mitotic (M) phase of the cycle by downregulating
the G2/M regulator String; (2) allows G1 entry by activating
Hec/CdhFrz, a regulator of the APC ubiquitination complex;
and (3) ensures S-phase entry by repressing Dacapo, a cyclinE/CDK
complex inhibitor. As the researchers discuss, a better understanding
of how external signalling pathways control physiological cell-cycle
transitions could identify how abnormal cell-cycle control occurs in
cancer cells.
Development 2004 131: 3169-3181.
First published online on 2 June 2004 as 10.1242/dev.01172
Schaeffer, V., Althauser, C., Shcherbata, H.R., Deng,W-M., and Ruohola-Baker, H. (2004) Notch-dependent expression of Fizzy-related/Hec1/Cdh1 is required for the mitotic-to-endocycle transition in Drosophila follicle cells, Curr. Biology, 14:630-636.
Findley SD, Tamanaha M, Clegg NJ, Ruohola-Baker H. (2003) Maelstrom, a Drosophila spindle-class gene, encodes a protein that colocalizes with Vasa and RDE1/AGO1 homolog, Aubergine, in nuage, Development 130, 859-71.
Deng, W-M, Schneider, M., Frock, R., Castillejo-Lopez, C. Gaman E.A., baumgartner, S., and Ruohola-Baker, H. (2003) Dystroglycan is required for polarizing the epithelial cells and the oocycte in Drosophila, Development 130, 173-184.
Deng, W-M, Althauser, C. and Ruohola-Baker, H. (2001) Notch-Delta signaling induces a transition from mitotic cell cycle to endocycle in Drosophila follicle cells, Development 128, 4737-4746.
Clegg, N., Findley, S., Mahowald, A.P. and Ruohola-Baker, H. (2001) Maelstrom is required to position the MTOC in stage 2-6 Drosophila oocytes. Dev Genes Evol. 211:44-48.
Jordan, K.J., Clegg, N.J., Blasi, J.A., Morimoto, A.M., Sen, J., Stein, D., McNeill, H., Deng, W-M., Tworoger, M. and Ruohola-Baker, H. (2000) The homeobox gene mirror links EGF signalling to embryonic dorso-ventral axis formation through Notch activation. Nature Genetics, 24, 429-433.
Deng, W-M. and Ruohola-Baker, H. (2000) Laminin A is required in the follicle cell-oocyte signaling which leads to the establishment of the anterior-posterior axis in Drosophila. Curr.Biol, 11:683-686.
Bryant,Z, Subrahnmanyan,L, LaTray,L., Li,M-J, van den Engh, G., and Ruohola-Baker,H. (1999) Characterization of differentially expressed genes in purified Drosophila follicle cells: towards a general strategy for cell type-specific developmental analysis. Proc Natl Acad Sci U S A. 96, 5559-5564.
Tworoger,M., Larkin,M.K., Bryant,Z., and Ruohola-Baker,H. (1999) Mosaic analysis in the Drosophila ovary reveals a common, Hedgehog inducible precursor stage for stalk and polar cells. Genetics, 151, 739-748.
Larkin,M.K., Deng,W-M, Tworoger,M., Holder,K., and Ruohola-Baker,H. (1999) Role of Notch pathway in terminal follicle cell differentiation in Drosophila oogenesis. DGE, 209, 301-311.
Clegg,N.J., Frost,D.M., Larkin,M.K., Subrahmanyan,L., Bryant,Z., and Ruohola- Baker,H. (1997) Maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA. Development, 124, 4661-4671.
Morimoto,A.M., Jordan,K.C., Tietze,K., Britton,J.S., O'Neill,E.M., and Ruohola- Baker,H. (1996) Pointed, an ETS domain transcription factor, negatively regulates the EGF receptor pathway in Drosophila oogenesis. Development 122, 3745-3754.
Larkin, M.K., Holder, K., Yost,C., Giniger,E., and Ruohola-Baker,H. (1996) Expression of constitutively active Notch arrests follicle cells at a precursor stage during Drosophila oogenesis and disrupts the anterior-posterior axis of the oocyte. Development 122, 3639-3650.
Ruohola-Baker,H., Jan,L.Y., and Jan,Y.N. (1994) The role of gene cassettes in axis formation during Drosophila oogenesis. Trends in Genetics, 10, 89-94
Clark,I., Giniger,E., Ruohola-Baker,H., Jan,L.Y., and Jan,Y.N. (1994) Microtubule polarity provides an anteriorposterior axis to the Drosophila oocyte. Current Biology 4, 289-300.
Ruohola-Baker,H., Grell,E., Baker,D., Jan,L.Y., and Jan,Y.N. (1993) Spatially localized rhomboid is required for establishment of the dorsal-ventral axis in Drosophila oogenesis. Cell 73, 953-965
Ruohola,H., Bremer,K.A., Baker,D., Swedlowe,J.R., Jan,L., and Jan,Y.N. (1991) Role of neurogenic genes in establishment of follicle cell fate and oocyte polarity during oogenesis in Drosophila. Cell 66, 433-449
Ferro-Novick,S., Newman,A.P., Groesch,M., Ruohola,H., Rossi,G., Graf,J., and Shim,J. (1991) An analysis of Bet1, Bet2, and Bos1. Three factors mediating ER to Golgi transport in yeast. Cell Biophysics 19, 25-33
Ferro-Novick,S., Newman,A.P., Groesch,M., Ruohola,H., Rossi,G., Graf,J., and Shim,J. (1990) Defining components, in vitro and in vivo, required for transport from the endoplasmic reticulum. AN Richards Symposium on Cellular Mechanism of Protein Sorting and Processing-Philadelphia 1990.
Groesch,M., Ruohola,H., Bacon,R., Rossi,G., and Ferro-Novick,S. (1990) Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast, J.Cell Biol., 111,45-55
Bacon,R.A., Salminen,A., Ruohola,H., Novick,P., and Ferro-Novick,S. (1989) The GTP-binding protein Ypt1 is required for transport in vitro: The Golgi apparatus is defective in ypt1 mutants. J. Cell Biol., 109,1015-1022
Walworth,N., Goud,B., Ruohola,H. and Novick,P. (1989) Fractionation of Yeast Organelles. Methods in Cell Biology, 31 (A.M. Tartakoff-ed.)
Ruohola,H., Kabcenell,A.K., and Ferro-Novick,S. (1989) Reconstitution of transport from ER to the Golgi complex in yeast using microsomes and permeabilized yeast cells. Methods in Cell Biology, 31 (A.M. Tartakoff-ed.)
Ruohola,H., Kabcenell,A.K., and Ferro-Novick,S. (1988) Reconstitution of protein transport from endoplasmic reticulum to the Golgi complex in yeast: The acceptor Golgi compartment is defective in the sec23 mutant. J. Cell Biol. 107,1465-1476.
Ruohola,H., Baker,S., and Platt,T. (1988) Orientation-dependent function of a short CYC1 DNA fragment in directing mRNA 3` end formation in yeast. Proc. Natl. Acad. Sci. U.S.A. 85,5041-5045.
Ruohola H, Ferro-Novick S. Sec53, a protein required for an early step in secretory protein processing and transport in yeast, interacts with the cytoplasmic surface of the endoplasmic reticulum. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8468-72. PMID: 3317409
Korhola,M., Liljeström,P., Kopu,H., Ruohola,H., and
Tokkeli,T. (1987) Structure and expression of Saccharomyces MEL 1 gene.
Biological Research on Industrial Yeasts. CRC Press, Inc. (Steward,G.-ed), 353.
Ruohola,H., Liljeström,P., Torkkeli,T., Kopu,H., Lehtinen,P., Kalkkinen,N.,
and Korhola,M. (1986) Expression and regulation of the yeast MEL 1 gene. FEMS
Microbiology Letters 34,179.
Nikula,P., Ruohola,H., Alhonen-Hongisto,L., and Jänne,J. (1985) Carnitine
prevents the early mitochondrial damage induced by
methylglyoxalbis(guanylhydrazone) in L1210 leukemia cells. Biochem.J. 228,513.
Seppanen P, Ruohola H, Janne J.
Ethylglyoxal bis(guanylhydrazone) as an inhibitor of polyamine biosynthesis
in L1210 leukemia cells. Biochim Biophys Acta. 1984 Apr
16;803(4):331-7.
Paulin,L., Ruohola,H.,
Nykänen,I., and Pösö,H. (1983) The incorporation of 1,3-diaminopropane into
thermine by an extreme thermophile: a model route for the biosynthesis of
polyamines. FEMS Microbiology Letters 19,299.