Treponema Laboratories
University of Washington • Department of Medicine

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Giacani L, Godornes C, Puray-Chavez M, Guerra-Giraldez C, Tompa M, Lukehart SA.TP0263 is a modulator of promoter activity of tpr Subfamily II genes of Treponmema pallidum. Mol Microbiol. 2009 April 30 ARTICLE

Brissette CA, Pham TT, Coats SR, Darveau RP, Lukehart SA. Treponema denticola does not induce production of common innate immune mediators from primary gingival epithelial cells.
Oral Microbiol Immunol. 2008 Dec; 23(6):474-81ARTICLE

Marra CM, Maxwell CL, Tantalo LC, Sahi SK, Lukehart SA.Normalization of serium rapid plasma regain titer predicts normalization of cerebrospinal fluid and clinical abnormalitites after treatment of neurosyphilis. Clin Infect Dis. 2008 Oct 1; 47(7):893-9. ARTICLE

Mulligan CJ, Norris SJ, Lukehart SA.Molecular Studies in Treponema pallidum Evolution: Toward Clarity? PLoS Negl Trop Dis. 2008 Jan 23; 2(1):e184. ARTICLE

Lukehart SA.Scientific monogamy: thirty years dancing with the same bug: 2007 Thomas Parran Award Lecture. Sex Transm Dis. 2008 Jan; 35(1)2-7. ARTICLE

Giacani L, Lukehart SA, Centurion-Lara A.Length of guanosine homopolymeric repeats modulates promoter activity of subfamily II tpr genes of Treponema pallidum ssp. pallidum. FEMS Immunol Med Microbiol. 2007 Nov; 51 (2):289-301ARTICLE

Pandori MW, Gordones C, Castro L, Engelman J, Siedner M, Lukehart S, Klausner J.Detection of azithromycin resistence in Treponema pallidum by real-time PCR. Antimicrob Agents Chemother. 2007 Sep;51(9):3425-30 ARTICLE

Godornes C, Leader BT, Molini BJ, Centurion-Lara A, Lukehart SA. Quantitation of Rabbit Cytokine mRNA by Real-Time RT-PCR. Cytokine 38:1-7, 2007. ARTICLE

Leader BT, Godornes C, Vanvoorhis WC, Lukehart SA. CD4+ Lymphocytes and IFN gamma Predominate in the Local Immune Responses in Early Experimental Syphilis. Infect Immun 75:3021-6, 2007. Article

Brissette CA, Lukehart SA. Mechanisms of Decreased Susceptibility to ß-defensins by Treponema denticola. Infect Immun 75, 2007. Article

Marra CM, Colina AP, Godornes C, Tantalo LC, Puray M, Centurion-Lara A, Lukehart SA. Antibiotic selection may contribute to increases in macrolide-resistant Treponema pallidum. J Infect Dis 194:1771-3, 2006. Article

Giacani L, Molini B, Godornes C, Barrett L, Van Voorhis Wesley, Centurion-Lara A, Lukehart SA. Quantitative analysis of tpr gene expression in Treponema pallidum isolates: differences among isolates and correlation with T-cell responsiveness in experimental syphilis. Infect Immun 75:104-12, 2007. Article

LaFond RE, Molini BJ, Van Voorhis WC, Lukehart SA. Antigenic variation of TprK V regions abrogates specific antibody binding in syphilis. Infect Immun 74:6244-51, 2006. Article

Leader BT, Van Voorhis WC , Lukehart SA. Expression of rabbit interleukin-4 and characterization of its biologic activity on T and B-cells. Vet Immunol Immunopathol 113:421-7, 2006. Article

Centurion-Lara A, Molini BJ, Godornes C, Sun E, Hevner K, Van Voorhis WC, Lukehart SA. Molecular differentiation of Treponema pallidum subspecies. J Clin Microbiol 44:3377-80, 2006. Article

LaFond RE, Centurion-Lara A, Godornes C, Van Voorhis WC, Lukehart SA. TprK sequence diversity accumulates during rabbit infection with Treponema pallidum subsp. pallidum, Nichols strain. Infect Immun 74:1896-906, 2006. Article

Cameron CE, Brouwer NL, Tisch LM, Kuroiwa JMY. Defining the interaction of the Treponema pallidum adhesin Tp0751 with laminin. Infect Immun 73: 7485-94, 2005. Article

Giacani L, Hevner K, Centurion-Lara A. Gene organization and transcriptional analysis of the tprJ, tprI, tprG, and tprF loci in Treponema pallidum strains Nichols and Sea 81-4. J Bacteriol 187:6084-93, 2005. Article

Giacani L, Sambri V, Marangoni A, Cavrini F, Storni E, Donati M, Corona S, Lanzarini P, Cevenini R. Immunological evaluation and cellular location analysis of the TprI antigen of Treponema pallidum subsp. pallidum. Infect Immun 73:3817-22, 2005. Article

Tantalo LC, Lukehart SA, Marra CM. Treponema pallidum strain-specific differences in neuroinvasion and clinical phenotype in a rabbit model. J Infect Dis 191:75-80, 2005. Article

Cameron CE, Brown EL, Kuroiwa JMY, Schnapp LM, Brouwer NL. Treponema pallidum fibronectin-binding proteins. J Bacteriol 186:7019-22, 2004. Article

Giacani LG, Sun ES, Hevner K, Molini BJ, Van Voorhis WC, Lukehart SA, Centurion-Lara A. The Tpr homologs in Treponema paraluiscuniculi, Cuniculi A strain. Infect Immun 72:6561-76, 2004. ARTICLE

Brissette CA, Simonson LG, Lukehart SA. Resistance to human ß-defensins is common among oral spirochetes. Oral Micro and Immunol 19:403-5, 2004. Article

Sun ES, Molini BJ, Barrett LK, Centurion-Lara A, Lukehart SA, Van Voorhis WC. Subfamily I Treponema pallidum repeat protein family: sequence variation and immunity. Microb Infect 6: 725-37, 2004. Article

Lukehart SA, Godornes C, Molini BJ, Sonnett P, Hopkins S, Mulcahy F, Engelman J, Mitchell SJ, Rompalo AM, Marra CM, Klausner JD. Macrolide resistance in Treponema pallidum in the United States and Ireland. New Eng J Med 351:154-8, 2004. Article

Centurion-Lara A, LaFond RE, Hevner K, Godornes C, Molini BJ, Van Voorhis WC, Lukehart SA. Gene conversion: a mechanism for generation of heterogeneity in the tprK gene of Treponema pallidum during infection. Molec Micro 52:1579-96, 2004. Article

Marra CM, Maxwell CL, Tantalo L, Eaton M, Rompalo AM, Raines C, Stoner BP, Corbett JJ, Augenbraun M, Zajackowski M, Kee R, Lukehart SA. Normalization of CSF abnormalities after neurosyphilis therapy: does HIV status matter? Clin Inf Dis 38:1001-6, 2004. Article

Marra CM, Maxwell CL, Smith SL, Lukehart SA, Rompalo AM, Eaton M, Stoner BP, Augenbraun M, Barker DE, Corbett JJ, Zajackowski M, Raines C, Nerad J, Kee R, Barnett SH. Cerebrospinal fluid abnormalities in patients with syphilis: association with clinical and laboratory features. J Infect Dis 189:369-76, 2004. - Article

LaFond RE, Centurion-Lara A, Godornes C, Rompalo AM, Van Voorhis WC, Lukehart SA. Sequence Diversity of Treponema pallidum subsp. pallidum tprK in human syphilis lesions and rabbit-propagated isolates. J Bacteriol 185: 6262-8, 2003. Article

Leader BT, Hevner K, Molini BJ, Barrett LK, Van Voorhis WC, Lukehart SA. The antibody response elicited against the Treponema pallidum repeat (Tpr) proteins differs during infection with different isolates of Treponema pallidum subsp. pallidum. Infect Immun 71:6054-7, 2003. Article

Cameron CE. Identification of a Treponema pallidum laminin-binding protein. Infect Immun 71:2525-33, 2003. Article

Morgan CA, Lukehart SA, Van Voorhis WC. Protection against syphilis correlates with specificity of antibodies to the variable regions of Treponema pallidum repeat protein K. Infect Immun 71:5605-12, 2003. Article

Van Voorhis WC, Barrett LK, Lukehart SA, Schmidt B, Schriefer M, Cameron CE. . J Clin Micro 41:3668-74, 2003. Article

Morgan CA, Lukehart SA, Van Voorhis WC. Immunization with the N-terminus of Treponema pallidum repeat protein K attenuates syphilitic lesion development in the rabbit model. Infect Immun 70:6811-6, 2002. Article

Morgan CA, Molini BJ, Lukehart SA, Van Voorhis WC. Segregation of B and T cell epitopes of Treponema pallidum TprK to variable and conserved regions during experimental syphilis infection. J Immun 169:952-7, 2002. Article

Brissette CA, Lukehart SA. Treponema denticola is resistant to human β-defensins. Infect Immun 70:3982-4, 2002. Article

O’Regan A, Castro C, Lukehart SA, Kasznica J, Joyce-Brady M. Barking up the wrong tree? Use of polymerase chain reaction to diagnose syphilitic aortitis. Thorax 57:917-8, 2002. Article

Centurion-Lara A, Sun ES, Barrett LK, Castro C, Lukehart SA, Van Voorhis WC. Multiple Alleles of Treponema pallidum repeat Gene D in Treponema pallidum isolates. J Bacterol 182:2332-5, 2000. Article

Centurion-Lara A, Godornes C, Castro C, Van Voorhis WC, Lukehart SA. The tprK is heterogeneous among Treponema pallidum strains and has multiple alleles. Infect Immun 68:824-31, 2000. Article

Cameron CE, Lukehart SA, Castro C, Molini B, Godornes C, Van Voorhis WC. Opsonic potential, protective capacity and sequence conservation of the Treponema pallidum subsp. pallidum Tp92. J Infect Dis 181:1401-13, 2000. Article

Kolman CJ, Centurion-Lara A, Lukehart SA, Owsley DW, Tuross N. Identification of Treponema pallidum subsp. pallidum in a 200-year-old skeletal specimen. J Infect Dis 180:2060-3, 1999. Article

Centurion-Lara, A, Castro C, Barrett L, Cameron C, Mostowfi M, Van Voorhis WC, Lukehart SA. Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response. J Exp Med 189:647-56, 1999. Article

Cameron CE, Castro C, Lukehart SA, Van Voorhis WC. Sequence conservation of glycerophosphodiester phosphodiesterase among Treponema pallidum strains. Infect Immun 67:3168-70, 1999. Article 

Arroll TW, Lukehart SA, Van Voorhis WC. T cell responses to Treponema pallidum subsp. pallidum antigens during the course of experimental syphilis infection. Infect Immun 67:4757-63, 1999. Article

Marra CM, Castro C, Kuller L, Dukes AC, Centurion-Lara A, Morton WR, Lukehart SA. Mechanisms of clearance of Treponema pallidum from the cerebrospinal fluid in a non-human primate model. Neurol 51:957-61, 1998. Article

Centurion-Lara A, Castro C, Castillo R, Shaffer JM, Van Voorhis WC, Lukehart SA. The flanking region sequences of the 15 kD lipoprotein gene differentiate pathogenic treponemes. J Infect Dis 177:1036-40, 1998. Article

Cameron CE, Castro C, Lukehart SA, Van Voorhis WC. Function and protective capacity of the Treponema pallidum subsp. Pallidum glycerophosphodiester phosphodiesterase. Infect Immun 66:5763-70, 1998. Article

Stebeck CE, Shaffer JM, Arrol TW, Lukehart SA, VanVoorhis WC. Identification of the Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase homologue. FEMS Microbiol Letters 154:303-10, 1997. Article

Centurion-Lara A, Castro C, Shaffer JM, Van Voorhis WC, Marra CM, Lukehart SA. Detection of Treponema pallidum by a sensitive reverse transcriptase polymerase chain reaction. J Clin Micro 35:1348-52, 1997. Article

Centurion-Lara A, Arroll TA, Castillo R, Shaffer JM, Castro C, Van Voorhis WC, Lukehart SA. Conservation of the 15 kd lipoprotein among Treponema pallidum subspeies, strains, and other pathogenic treponemes: Genetic and antigenic analyses. Infect Immun 65:1440-4, 1997. Article

VanVoorhis WC, Barrett LK, Nasio JM, Plummer FA, Lukehart SA. Lesions of primary and secondary syphilis contain activated cytolytic T cells. Infect Immun 64:1048-53, 1996. Article

VanVoorhis WC, Barrett LK, Koelle DM, Nasio JM, Plummer FA, Lukehart SA. Primary and secondary syphilis lesions contain mRNA for Th1 cytokines. J Infect Dis 173:491-5, 1996. ARTICLE

Marra CM, Longstreth WT, Maxwell CL, Lukehart SA. Resolution of serum and cerebrospinal fluid abnormalities after treatment of neurosyphilis: Influence of concomitant human immunodeficiency virus infection. Sex Trans Dis 23:184-9, 1996. Article 

Cummings MC, Lukehart SA, Marra CM, Smith BL, Shaffer J, DeMoele R, Castro C, McCormack WM. Comparison of methods for the detection of Treponema pallidum in lesions of early syphilis. Sex Trans Dis 23:366-9, 1996. Article 

Centurion-Lara A, Castro C, Van Voorhis WC, Lukehart SA. Two 16S-23S ribosomal DNA intergenic regions in different Treponema pallidum subspecies contain tRNA genes. FEMS Microbiol Letters 143:235-40, 1996. Article 

Marra CM, Critchlow CW, Hook EW III, Collier AC, Lukehart SA. Cerebrospinal fluid treponemal antibodies in untreated early syphilis. Arch Neurol 52:68-72, 1995. Article 

Gordon SM, Eaton ME, George R, Larsen S, Lukehart SA, Kuypers J, Marra C, Thompson S. Response of symptomatic neurosyphilis to high dose intravenous penicillin G in persons infected with human immunodeficiency virus. New Engl J Med 331:1469-73, 1994. Article 

Shaffer JM, Baker-Zander SA, Lukehart SA. Opsonization of Treponema pallidum is mediated by IgG antibodies induced only by pathogenic treponemes. Infect Immun 61:781-4, 1993. ARTICLE

Lukehart SA, Shaffer JM, Baker-Zander SA. A subpopulation of Treponema pallidum is resistant to phagocytosis: Possible mechanism of persistence. J Infect Dis 166:1449-53, 1992 ARTICLE