Greenberg Lab masthead
School of MedicineUniversity of Washington • Box 357735 • 1705 NE Pacific St • Seattle WA 98195
 

UW CFF RDP Project: Epithelial-leukocyte interactions in regulation of pulmonary inflammation in cystic fibrosis

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Principal Investigator: John McGuire, M.D.

The objective of this project is to evaluate the potential role of regulatory T-cells (Tregs) in the control of pulmonary inflammation induced by P. aeruginosa infection. We proposed that interactions between the adhesion protein E-cadherin and the leukocyte-specific E-cadherin receptor CD103 activate CD103+ dendritic cells to induce a Treg response and an increase in anti-inflammatory IL-10 in the lung after P. aeruginosa infection and hypothesized that disruption of this mechanism in CF could contribute to low IL-10 levels and chronic inflammation in lungs of CF patients. We proposed three aims: (1) Define the role of CD103 and Tregs in P. aeruginosa pneumonia in mice. (2) Evaluate whether Treg numbers and function are impaired in CF mice. (3) Validate these concepts by assessing samples of lung tissues from CF patients.

 

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Cycstic Fibrosis Foundation University of Washington