Organic bioelectronics refers to the coupling of conducting polymer based devices with biological systems, proven repeatedly in the last decade to provide numerous advantages to a wide variety of biomedical applications in terms of sensitivity, specificity and most importantly, bridging of the biotic/abiotic interface. We focus on the unique properties of organic electronic materials that allow easy processing, and flexibility in design as well as chemical tunability, to develop state-of-the-art tools to (1) develop relevant in vitro models by creating more ‘in vivo’ like environments and (2) monitor cells i.e. for diagnostic purposes following exposure to toxins or pathogens. We have successfully demonstrated the use of the organic electrochemical transistor (OECT) for monitoring in vitro models of the gastrointestinal tract, the kidney and the blood brain barrier. For each application, we attempt to recreate the in vivo conditions through the use of microfluidics, biofunctionalised materials, combinations of different cell types, while simultaneously designing the materials/devices in the most appropriate form factor to suit the model at hand. Our goal is to develop physiologically relevant in vitro models with integrated monitoring systems that obviate the need for animal experimentation in diagnostics, toxicology or drug development. In this presentation, I will focus on new work that we have carried out to increase the sensitivity of our devices for monitoring a broader selection of tissues in vitro, integration of our devices with cells in 3D formats, and finally, inclusion of multi-parameter monitoring by additional functionalities such as metabolite sensing and high resolution optical imaging into our devices.