Quellos High Throughput Screening Core
Small Molecule Screening
High throughput screening (HTS) of chemical (small molecule) libraries against cells or classical biochemical targets has been the mainstay of the pharmaceutical industry generating drug leads that can be further optimized for "first-in-man" clinical studies. The Quellos High Throughput Screening Core's Small Molecule Library currently contains more than 115,000 separate chemical entities selected for their "drug-like" behaviors and diverse coverage of the bioactive chemical or pharmacophore space. We are currently offering services for assay development, optimization and screening programs (called the "Informer Screen" and the "Secondary" screen) that are directed towards the Quellos High Throughput Screening Core's libraries of small molecules.
Chemical Library
The primary portion of our small molecule library was acquired from ChemBridge Corporation. Initially it will contain approximately 115,000 separate entities at 10mM concentration in 100% DMSO and stored in 96 well plates (72 compounds per plate, ~1,600 plates). DIVERSet is 50K subset of compounds from the library that corresponds to our "Informer Screen." Secondary Screens will be performed on a subset of the remaining library (>64K compounds) corresponding to ChemBridges' CNS-Set, KINASet and ION Channel Set. Other facility collections will be included in the derivation of the screening subset.
Another collection of our library is the NCI Diversity Set #1 that was derived from a Developmental Therapeutics Program (DTP) at the National Cancer Institute, National Institute of Health. The set contains nearly 2,000 entities derived from the original library of 140,000 compounds created by submission from external investigators worldwide or DTP directed syntheses. This set was recently obtained and added to the Quellos library and will be made available for screening and/or cherry picking.
Future planned acquisitions include:
- Inclusion of 1824 compounds that are representative of a larger library of 20,000 peptide mimetic compounds from a Commercial Enterprise…
- 1-2K known drugs, experimental bioactives and pharmaceutically active compounds
Contact:
Timothy J. Martins, Ph.D.
Co-Director
Phone: 206-616-3115
E-mail: tmartins@uw.edu
