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ben101120032003@yahoo.com.cn Education BS Biology, Sichuan University 2007 Research summary Lab work in Carl Anthony Blau’s lab in the School of Medicine (Hematology), University of Washington The projects I am highly involved with include:
Methods such as quantitative rtPCR, western blot, multi-color flow cytometry etc are extensively practiced. Basic molecular experiments including transformation, maxiprep, miniprep, extraction of genomic/plasmid DNA, RNA from different sources are also broadly carried out. Lentivirus production for gene therapy(transduction&transfection) is done nicely. Notably, I got full training in purifying CD34+ cells from the cord blood and dog bone marrow (also linage depletion for bone marrow) and have been in charge of the purifying work with satisfying results. Different mouse work such as intraperitoneal injection, tail vein injection, blood collection from orbital sinus in the secured mouse facility of UW has been successfully conducted after rigorous training and exams. Comprehensive tissue culture work has been practiced including culturing tumor cells/human ES cells. Cell growth/drug inhibition tests are done by MTS assay and regular staining. Fundamental hematology work WBC, RBC, platelets, hematecrits count/cytospin/ smear staining (Wright‘s and Giemsa’s) is carefully done. Besides, I have been taking an active part in weekly lab meetings and other relevant meetings such as Gene Therapy Club including presenting updated research achievements and cutting-edge breakthroughs in related fields. My selection of certain articles has been adopted in one of the latest grant in the lab. (The critically important issue was first presented by me in one of the lab meetings) Honors Poster
Highlights 2005.9 Volunteering in the ASUW(The Associated Students of the University of Washington) DAWG DAZE 2005.10-2006.6 Volunteering for three quarters in the Education Outreach Program of Fiuts(Foundation for International Understanding Through Students) in UW(including writing to and meeting 5 pen pals from local elementary schools)in different quarters 2006. 1 Volunteering in the community service on Martin Luther King, Jr. Day (group project included the thorough cleaning of a blind man’ s house in the Seattle area) 2006.2 Written paragraph on New Year luck published in the Northwest Asian Weekly 2006.2 Being one of the emcees of the Spring Festival Celebration (with over 800 attendants) and performed a traditional Chinese Cross Talk 2006.4 Being one of the group leaders from UW in welcoming the visit of Chairman Hu Jingtao to Seattle and meeting with the Chief Consulate from San Francisco Life is short
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