RESEARCH and ACTIVITIES

January 1, 2003 — December 31, 2003

Graduate Students

Susan Grose continued her study on the phylogenetic analysis of two groups in the Bignoniaceae (the tribe Crescentieae and the genus Tabebuia). She is looking at the evolutionary relationships of these groups and examining the development of the unilocular, indehiscent fruit characteristic of the Crescentieae, which differs from the bilocular, dehiscent fruit typical of the rest of the family. She has been databasing specimens from large loans from institutions including the Smithsonian, the Missouri Botanical Garden, and the New York Botanical Garden. In 2003, Susan was awarded a Doctoral Dissertation Improvement Grant from the National Science Foundation in support of her ongoing research. Susan was lead Teaching Assistant for Plant Classification and Identification (Bot. 113) in Spring Quarter, and was Course Instructor for Bot. 113 in Summer Quarter. Additionally, Susan received the Ingrith Dreyup Olsen Teaching Award. She presented a talk at Botany 2003 in Mobile, AL entitled ,"Patterns of Evolution in neotropical Bignoniaceae: Relationships within Crescentieae". Susan was also involved with public outreach at the Kennidale Elementary School where she spoke to students about her work as a botanist.

Suzanne Joneson completed her Master's of Science in Botany in Joe Ammirati's lab, where she studied the phylogeny of the Ramalina almquistii complex. In August, she began her doctoral research in lichenology at Duke University with Dr. Francois Lutzoni. Suzanne has ongoing work with Dr. Katherine Glew to study and map the distribution of the lichen taxa Thamnolia subuliformis and Thamnolia vermicularis in North America.

P. Brandon Matheny completed his doctoral research in Joe Ammirati's and Ben Hall’s labs on the

molecular phylogeny and taxonomy of the mushroom genus Inocybe (Agaricales, Basidiomycota) using RPB1, RPB2, and nuclear large subunit rDNA genes. The taxonomic component of his work relied on comparing fresh material with dried specimens from the Herbarium and those on loan from other institutions. Sources of DNA extraction included the dried fruitbodies from specimens that are housed at WTU and those borrowed from other herbaria. The scope of his dissertation included an effort to sample globally among species of Inocybe, including North America, Europe, temperate Asia, southeast Asia, the neotropics, temperate areas of South America, the paleotropics of Africa, New Zealand, and Australia. As a result of this broad sampling, many new species have been discovered: (1) four new neotropical species that associate with the caesalpinioid tree, Dicymbe corymbosa, from Guyana; (2) two new species of Inocybe from northern California and Arizona; (3) and, remarkably, about thirty new species of Inocybe from New South Wales and Western Australia. Taxonomic and phylogenetic emphasis was placed on the subgenus Mallocybe, which is discovered to encompass two separate major clades, both of which will be proposed at the genus rank. Brandon has also recently participated in the description of a new mushroom species from the genus Cortinarius, C. helodes, from the Issaquah plateau just east of Seattle.

Brandon accepted a post-doctoral position with Dr. David Hibbett at Clark University in Worcester, MA, where he is collaborating on the NSF-sponsored Assembling the Fungal Tree of Life project.

Valerie Soza began her doctoral research in the lab of Dick Olmstead in Fall 2003. Valerie came to UW from the Rancho Santa Ana Herbarium where she was responsible for the rare plant monitoring program. Valerie is in the process of selecting her research topic.

David Tank is conducting a phylogenetic analysis of the subtribe Castillejinae (Orobanchaceae). By using DNA sequence data from variable chloroplast and nuclear ribosomal DNA regions, he will be able to test hypotheses of monophyly in Castillejinae, and to determine relationships within and among the main lineages of Castillejinae. David is also using phylogenetic analyses to assess interspecific relationships within Castilleja, a genus renowned for its morphological complexity. He is interested in determining the relative contributions of polyploidy, hybridization, and rapid/recent radiation to this morphological complexity. David is also working on a side project examining the phylogenetic position of Nesogenes based on cpDNA ndhF data, and is preparing two papers for publication based on his Castilleja research.

David attended the 50th Annual Systematics Symposium at the Missouri Botanical Garden in St. Louis, MO.

2003 was a very active field season for him that included participation in the Herbarium Foray to the Okanogan National Forest in Washington, and collecting of Castilleja in Oregon, Idaho, and Costa Rica. In these travels, David collected voucher specimens, silica-dried leaf material for DNA extraction, and flower buds for cytological studies. His research has also required extensive sampling in the Herbarium, where he has collected tissue from taxa within the genera Orthocarpus, Cordylanthus, and Tryphysaria, and from additional Castilleja taxa. David received the 2003 Delzie Demaree Travel Award ($250), which is given to one graduate student each year to aid in travel expenses for attending a scientific meeting. He also submitted a Doctoral Dissertation Improvement Grant to the National Science Foundation. David served as a Teaching Assistant for Biology 441 (Anatomy and Morphology of Land Plants), and Biology 117/317 (Plant Identification and Classification). He also led a field trip for Biology 180 to Perry Creek to study fern and bryophyte diversity.

Research Associates

Dr. Michelle Seidl continued her research on fungal taxonomy, with emphasis on the genus Cortinarius. Michelle completed work on a three-year grant that she received from the United States Department of the Interior, Bureau of Land Management (BLM). In this work Michelle identified Cortinarius/Dermocybe specimens, and gilled and non-gilled Basidiomycetes, excluding corals, as part of the Northwest Forest Plan and the Survey & Manage Program in Washington and Oregon. Her duties included identification, photography and descriptions of potential and listed species, drying specimens, and packaging and sending specimens to the Forest Service Mycology Team in Corvallis, Oregon.

Michelle participated in a number of professional activities in 2003. She continued her work with the Pacific Northwest Key Council, and in May participated in the preliminary meeting of Pacific Northwest Fungal Database Project at UW's Pack Forest, near Eatonville, WA. Michelle served as journal editor or peer reviewer for publications in Mycotaxon, Mycologia, and Fremontia. She was also involved in a number of public outreach efforts that included commenting on SEIS for the BLM/Forest Service's Northwest Forest Plan, consulting as a fungal expert for the BLM on global and state ranking strategies for fungi, serving as a Volunteer Identification Consultant for Mushroom Poison Cases, and the North American Mycological Association (NAMA), and serving as a volunteer mushroom identifier for annual mushroom shows organized by the Puget Sound Mycological Society, and the Kitsap Peninsula Mushroom Association.

Mark Egger conducts research on the genus Castilleja, and will author the treatment of this genus for the Flora of North America project. Mark is working towards a monographic revision for the entire genus. Over the past year, he focused his efforts on the Castilleja of Mexico, and annotating specimens on loan from several herbaria, along with a number of smaller packets of "gifts for ID" from other institutions and individuals. A recent focus has been the documentation and verification of Castilleja type collections, and the corresponding preparation of a fully annotated synonymy for the entire genus. Through his work on loans this past year, Mark identified a sizeable number of previously undocumented type specimens from other institutions. He identified over 30 such types, including several holotypes, from specimens borrowed from the Herbarium of the University of Lyon, France.

Mark's fieldwork included an April trip to California where he collected Castilleja in the central and north-western regions of the state. In August, he traveled to Mexico where he collected in seven states in the west-central portion of the country. Mark conducted herbarium-based research at the University of California-Berkeley (UC/JEPS), California Academy of Science (CAS), and the IEB Herbarium in Patzcuaro, Michoacan.

Dr. Katherine Glew researches rare lichens species of Washington, alpine lichens, and lichen biodiversity of Far East Russia. She served as a technical advisor to the Washington Department of Natural Resources for the updated publication of the Natural Heritage Program's "Rare lichens for Washington State". She also provided technical assistance to the U.S. Forest Service regarding rare lichens species collected as part of the Survey & Manage program. Katherine taught biology for the University of Puget Sound in the spring quarter, and received an Enrichment Grant from the University of Puget Sound for her research project "Secondary Compounds found in Alpine lichens from Washington State using Thin-layer Chromatography". She also attended the Northwest Science Annual Meeting at Boise State in Idaho, where she gave a talk entitled, "Alpine Lichens from Mount Rainier National Park, Washington State, USA".

In July and August, Katherine represented the WTU on the NSF-supported biotic survey of the Sakhalin Island in Far East Russia, where she collected lichens. She served as journal editor and peer reviewer for The Bryologist (Journal for the American Bryological and Lichenological Society), and Northwest Science (Journal for the Northwest Scientific Association). She also serves as Board Member for both of these scientific organizations. Katherine was also involved in public outreach, leading a lichen field trip to the Hylebos Wetlands in Federal Way, WA for the Washington Native Plant Society, Central Puget Sound Chapter

Dr. Linda Ann Vorobik continued her ongoing revisionary work on Arabis for the Oregon Flora Project. Her particular focus is the purple-flowered A. macdonaldiana complex. Preliminary ITS sequence work has been completed on these and related taxa and will be contributed to a paper on Arabis phylogeny led by Dr. Bitty Roy. She is also conducting research on the reproductive biology of Arabis, and the post-Biscuit Fire vegetation of Babyfoot Lake Botanical Area in Josephine County, Oregon .

Linda is instrumental to the operations of several major native plant endeavors. She served as Illustrations Editor for the Manual of Grasses of the Continental United States and Canada, Mary Barkworth, editor (out of Utah State University, as part of the Flora of North America project), published in 2003. Linda also serves as Editor of the California Native Plant Society's journal Fremontia. She is authoring a book entitled Sierra Nevada Plants, Wildflowers to Trees, with photographer Stephen Sharnoff. Additionally, Linda serves as Illustrator for the forthcoming books Flora of San Nicolas Island, and Flora of Santa Catalina Island. Linda's teaching efforts include "Crash Course in Vascular Plant Families" for the Siskiyou Field Institute, and "Basic Botanical Illustration" for Jepson Workshops, at the University of California at Berkeley's Jepson Herbarium.

Peter Zika was involved in a wide range of research on Pacific Northwest floristics. The focus of his efforts this year included the genera Botrychium, Cardamine, Carex, Impatiens, Prunus, Juncus, Crataegus, and Cotoneaster. In support of this research, Peter visited herbaria throughout North America, including the New York Botanical Garden, Harvard University's Gray Herbaria, North Cascades National Park, Olympic National Park, University of British Columbia, Oregon State University, and the Rancho Santa Ana Botanical Garden. Peter is also studying bird dispersal of introduced vascular plants in the Pacific Northwest.

Peter served as manuscript editor for American Fern Journal and Madroño, and reviewed several conservation plans for the US Forest Service and New England Wildflower Society. Peter taught a course on invasive knotweed identification for King County Noxious Weed Control Board, and attended a course in sedge taxonomy and ecology at Eagle Hill, Maine that was taught by the University of Michigan's Dr. A. A. Reznicek . His work with the National Park Service to document the flora of Crater Lake resulted in a book published in 2003. During 2003, Peter made over 1,100 collections, and most of these specimens were donated to the University of Washington Herbarium. Some of the places outside of the Pacific Northwest where Peter collected were Arizona, Utah, Colorado, Maine, Québec, New Brunswick, Massachusetts, Chile, and Hawai'i

Staff

David Giblin served as mentor for two Advanced Program Biology students from Seattle's Franklin High School. In addition to training the students in Herbarium management practices, David oversaw the students' development and completion of individual projects based on their experience in the Herbarium. The project of one of the students, Margareth Tran, won a countywide science fair at the end of the program. In February, he established a collaboration with Dr. Peter Dunwiddie of The Nature Conservancy to conduct botanical inventories on TNC properties in Washington State. In March, David coordinated a $10,000 facilities improvement project to the Herbarium that included the installation of new research benches and ethernet ports. David organized and led three overnight collecting trips in 2003 to TNC's Ellsworth Creek Preserve in southwestern Washington. David also organized and participated in one-day collecting trips in April, May, and September to gather new county records for WTU's holdings. Other attendees on these trips were WTU's Ben Legler, WTU Research Associate Peter Zika, and WTU volunteer Don Knoke.

David worked with the National Park Service to obtain additional funds ($20,000) in support of ongoing botanical survey work at national parks in the Pacific Northwest. The funds supported one collecting trip to North Cascades National Park in August, with additional trips to Ebey's Landing National Historic Reserve, and Mount Rainier and North Cascades national parks scheduled for 2004. In July, David and Dick Olmstead submitted a $500,000 grant proposal to the National Science Foundation's Biological Research Collection program. The proposal requests funds to database over 200,000 western North America vascular plant specimens, upgrade existing specimen storage equipment, and hire a curator for the bryophyte and lichen collections. In July, David organized and led the eight annual Herbarium Foray, which took place July 24- 28 in the Okanogan National Forest. The Foray was attended by 25 amateur and professional botanists.

David was a guest lecturer in Spring and Summer quarters for Botany 113. He continued his service on the Editorial Board of the Washington Native Plant Society, provided training to volunteers from People for Puget Sound on plant collecting techniques, and gave a lecture to the Snohomish County Native Plant Steward Training program on basic plant morphology. David oversaw undergraduate student Scott Anderson's Herbarium practicum in Fall Quarter. Scott received training in all phases of Herbarium management, and worked with TNC specimens to gain experience in the identification of graminoids. David is also co-advising Lan Ting, an undergraduate student in the UW-Sichuan University exchange program, who is conducting research on hybridization in native and non-native species of Impatiens. David is collaborating with Drs. Kern Ewing and Kee Dae Kim in the College of Forestry on a research project studying the efficacy of willow stake plantings on controlling invasive reed canarygrass (Phalaris arundinacea). In September, David assumed responsibility for the management of the Erna Gunther Ethnobotanical Garden at the entrance to the Burke Museum.

Sarah Gage continued as Project Coordinator for the ongoing botanical survey project conducted with the National Park Service. Sarah led a four-day trip with support from National Park Service staff and six WTU volunteers to the Fisher Creek Basin in North Cascades National Park, and assisted with identifying these specimens in the Herbarium. Photos that she took during previous North Cascades National collecting trips were published in Time Magazine's supplement, "Time For Kids". Sarah continued processing plants that she collected on the International Kuril Island Project (IKIP), and participated in the Herbarium's Okanogan National Forest Foray. During the summer, she worked as a Paleobotany Research Assistant with the Evolving Earth Foundation, morphotyping fossils collected in British Columbia. In August, she collected plants in support of a Lewis and Clark exhibit being developed at Fort Canby State Park. Sarah led a plant taxonomy and plant keying session in September for the Snohomish County Washington Native Plant Society Native Plant Stewardship program.

In May, Sarah earned her Master of Fine Arts degree in Creating Writing from the University of Arizona (UA). In addition to her degree, Sarah received the 2003 Intro Journals Award from the Associated Writing Programs at UA, and was a finalist in the Writers at Work Fellowship Competition in Salt Lake City, Utah. She was also the recipient of the UA's 2003 John Weston Scholarship in Creative Writing. She delivered an invited seminar at UA on teaching the persuasive research paper unit, was a Manuscript Screener for UA's Poetry Center Residency Competition, and taught First-Year Composition. Here in Seattle, Sarah taught Introduction to College Writing in the fall at Edmonds Community College.

Ben Legler continued his work on developing WTU's vascular plant database and Web site. Ben also developed databases for the lichen and fungal collections, the latter of which he demonstrated for the Pacific Northwest Fungi Project workshop at Pack Forest May 16-18. He also worked with Fred Weinmann to create an online county-level Carex atlas for Washington using data from seven Pacific Northwest herbaria, and is currently working with Fred and Peter Zika to create more detailed and functional specimen dot maps for the Carex Atlas. Ben made several individual collecting trips to counties throughout the state, which yielded several hundred new county records for the WTU vascular plant collections. For part of the summer, he was a Field Botanist on a research project led by Dr. Cara Nelson in the College of Forest Resources. He represented WTU in the final year of the ISIP biotic survey and inventory project, where he made over 700 collections during his seven weeks on Sakhalin Island. Ben also completed the databasing of the 2002 North Cascades National Park project specimens, and wrote the final report summarizing the year's activities.

Sharon Rodman was contracted by the National Park Service (NPS) to create a database of label information from WTU specimens collected on NPS land. By federal law, all plants collected on NPS land after April 1984 belong to the NPS, however they are on permanent loan to the herbaria which currently house the specimens. The work is part of an on-going inventory of plants collected in parks of the North Coast and Cascade network, which includes Ebey’s Landing National Historic Reserve, Fort Clatsop National Memorial, Fort Vancouver National Historic Site, North Cascades National Park Service Complex, Mount Rainier National Park, Olympic National Park, and San Juan National Historic Park. In July Sharon participated in the Herbarium Foray to the Okanogan National Forest, and in the fall served as Botany Instructor for Bastyr University.

Faculty

Joe Ammirati, chair of the Department of Botany, continued his long-term research on the taxonomy of the mushroom genus Cortinarius in North and Central America, with emphasis on the boreal and montane species of the Pacific Northwest and the Rocky Mountains. His main collaborators are Meinhard Moser (University of Innsbruck) and Michelle Seidl. He also works with Roy Halling (New York Botanical Garden) on fungi of Costa Rica and with Greg Mueller (Field Museum). Other long-term interests include the taxonomy and distribution of agaric species and macrofungi of the Pacific Northwest, and species richness and fungus diversity in conifer ecosystems in the Olympic and Cascade Mountains. Joe is actively involved with the Mycological Society of America (MSA), serving the MSA in a variety of ways: as a member on both the International Committee and the Biodiversity Committee, and as Co-Chair of the Awards Committee. He was a peer-reviewer for the journals Mycotaxon, Mycologia, and Canadian Journal of Botany.

Eugene N. Kozloff, Professor Emeritus of Zoology, is currently working on a flora of Oregon, Washington, and British Columbia west of the Cascades. The anticipated publication date is early 2005. Permanently based at Friday Harbor Laboratories, Gene visits the Herbarium frequently to examine specimens and to select others for additional study at Friday Harbor. He has used over 2,000 WTU specimens to develop dichotomous identification keys in the book and to locate plants in the field. The book will be substantially complete for the region, and will be useful to amateurs, non-specialist professionals in various kinds of environmental work, and students in courses on plant classification and identification. Its general organization includes keys to families, genera, and species, along with over 700 color photographs and line drawings. The book will be similar to that of Beidleman and Kozloff: Plants of the San Francisco Bay Region, revised edition, 2003, published by University of California Press. His work in the Herbarium has been generously aided by specialists who work there.

Arthur R. Kruckeberg, Professor Emeritus of Botany, continued his long-term efforts on the floristics and ecology of unusual substrates, especially serpentines. Art has three ongoing book projects: "Plant Hunters of the Pacific Northwest", "Natural History of Sagebrush Country", and "Wildflower Hikes in Washington". He continues to conduct research on serpentine tolerance of western North America vascular plants, and seed germination of plants native to the Pacific Northwest. Art gave lectures on the geology of plant life for the Northwest Geological Association in Seattle, on Puget Sound natural history for the town of Port Townsend, WA, for the Snohomish County Native Plant Stewards Training Program, for Seattle Central Community College, and for the Lummi Indian community. He traveled to Havana, Cuba and Stanford University where he delivered talks on the preservation of serpentine areas in North America. In addition, Art led field trips to the Wenatchee Mountains for the British Fern Society, and to the Methow Valley and Mount Adams for the North Cascades Institute.

Richard Olmstead, Professor of Botany in the Biology Department, Herbarium Curator, and Burke Museum Curator of Botany, has projects ongoing in the macrosystematics of Scrophulariaceae, Lamiaceae, Boraginaceae, Bignoniaceae, as well as basal land plants. He received a $6,000 REU (Research Experience for Undergraduates) supplement to his NSF-supported research into seed plant and basal angiosperm phylogeny (NSF Research Grant: "Chloroplast DNA Phylogeny of Seed Plants and Basal Angiosperms" DEB- 0090313. 2001-2004. $240,000). Dick continued work on collaborative projects with scientists at UW and other institutions (NSF Research Grant 2002-2007: "The Plant and Algal BAC Library Project: A Fundamental Public Resource for Studying Evolution, Physiology and Development." (D. Mandoli, Project Director, R. Olmstead, C. dePamphilis, R. Wing, J. Banks, Co-PI, and NSF Research Grant 2002-2007 ATOL: Deep Green Plant Phylogenetics: Novel Analytical Methods for Scaling Data from Genomics to Morphology." DEB-0228660, UW budget: $642,000; Co-PI with D. Mandoli).

In February, Dick traveled to Puerto Rico to where he visited herbaria at the University of Puerto Rico (UPRRP) and the Puerto Rico Botanical Garden (UPR), and collected plants throughout the island. He traveled to CalState - Chico in June to attend the jointly held annual meetings of the Society of Systematic Biologists/Society for the Study of Evolution. In July, he co-led the Eighth Annual UW Herbarium Foray to the Okanogan National Forest in northern Washington. He attended the Missouri Botanical Garden's annual systematics symposium in October, and visited the Garden's herbarium as well. Dick was a contributor to two talks at the jointly held annual meetings of the American Society of Plant Taxonomists/Botanical Society of America ("Patterns of evolution in Neotropical Bignoniaceae: relationships within Crescentieae", with Susan Grose; "Molecular phylogenies from nuclear and chloroplast genes support the connection of the Macronesian genus Bystropogon (Lamiaceae) to the radiation of the New World Mentheae" with J. Trusty, D. Bogler, A. Santos-Guerra, and J. Francisco-Ortega). Dick also hosted Drs. Jack Sullivan from the University of Idaho, and Quentin Cronk from the University of British Columbia Botanical Garden during their visits to UW as invited speakers in the Biology Department seminar series.

Dick taught Plant Molecular Systematics and Evolution (Bot 527) during winter, spring, and fall quarters, and Plant Identification and Classification (Bot 113) in spring quarter. He reviewed 12 manuscripts for each of eight different journals, one treatment for Families and Genera of Flowering Plants, one book chapter on asterid systematics, and 12 NSF and one National Geographic Society grant proposals. Dick also served as a panel member for NSF's Tree of Life Program. He served on the German Research Panel for "Phylogenetic Radiations" in Bad Honnef, Germany, which included the review of 12 grant proposals. Additionally, Dick participated in local public outreach by teaching class sessions for the Washington Native Plant Society Native Plant Steward training programs.