Fluorescence Resonance Energy Transfer (FRET)

FRET is an elegant experimental tool to examine the spatial relationship between two proteins in vivo.

This page is the first in a series of linked web pages that describe our approach. The pages include

• Introduction to FRET
• Microscope set up
• Preparation of the yeast
• Mounting cells
• Image acquisition
• Image analysis-Spillover factors
• Determine FRETR values

To skip to a section click on the link.