FRET-Image acquisition end

The precise details for acquistion will depend on the microscope. We illustrate with screen captures from our DeltaVision microscope running Softworx. Our typical experimental protocol is shown here. We image with the 100X objective, the camera is set for 2 x 2 binning, 3 sequential 0.4 second exposures capture the YFP, FRET and CFP channels and finally a 0.05 second DIC image is captured.

Microscope Settings	Acquisition Parameters