An Introduction to Mass Spectrometry (cont.)

IV. Determining the Amino Acid Sequence of a Peptide

By using tandem mass spectrometry, data specific to an individual peptide is collected. Fragmentation information can be used to determine the amino acid sequence of a peptide.

Shown is the manner in which peptides fragment by the bonds that have been observed to dissociate. The most common fragment ions are the b and y-type ions, which provide overlapping information about the sequence.

b ions <-------------------------------------> y ions

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