L broth and L plates
1. Tryptone 2. Yeast Extract 3. Sodium Chloride 4. 10N NaOH to pH 7.5 For plates only: 5. Agar For plates and Broth: 6. Autoclave and cool to 50¡C. |
1 L 10 g 5 g 5 g 14 g |
1.5 L 15 g 7.5 g 7.5 g 21 g |
2 L 20 g 10 g 10 g 28 g |
NZY+ broth
|
1 L |
1. NZ amine (casein hydrolysate) |
__ 10 g |
2. yeast extract |
__ 5 g |
3. NaCl |
__ 5 g |
4. MgSO4 |
__ 2 g |
5. 1 M MgCl2 |
__ 12.5 ml |
6. Aliquot ~100 mls per 200 ml bottle |
|
7. Autoclave |
|
8. Add 50% glucose |
__ 0.8 ml per bottle |