L broth and L plates

 

 

1. Tryptone

2. Yeast Extract

3. Sodium Chloride

4. 10N NaOH to pH 7.5

 

For plates only:

5. Agar

 

For plates and Broth:

6. Autoclave and cool to 50¡C.

 

1 L

 

      10 g

      5 g

      5 g

 

 

 

      14 g

 

1.5 L

 

      15 g

      7.5 g

      7.5 g

 

 

 

       21 g

 

2 L

 

      20 g

      10 g

      10 g

 

 

 

     28 g

 

 

 

NZY+ broth

 

1 L

1. NZ amine (casein hydrolysate)

__ 10 g

2. yeast extract

__ 5 g

3. NaCl

__ 5 g

4. MgSO4

__ 2 g

5. 1 M MgCl2

__ 12.5 ml

6. Aliquot ~100 mls per 200 ml bottle

 

7. Autoclave

 

8. Add 50% glucose

__ 0.8 ml per bottle