Minimal (M9) plates

1. H2O 2. M9 Salts (see below) 3. Agar 4. Autoclave and cool to 50°C 5. 1M MgSO4 6. 1M CaCl2 7. 50% Glucose 8. 1% Thiamine 9. 50 mg/ml Ampicillin (optional)

500 ml 450 ml 50 ml 15 g 1 ml 0.1 ml 4 ml 0.25 ml 0.5 ml

1 L 900 ml 100 ml 30 g 2 ml 0.2 ml 8 ml 0.5 ml 1 ml

M9AA: salt mix

1. Na2HPO4 2. KH2PO4 3. NaCl 4. NH4Cl 5. Autoclave

1 L 60 g 30 g 5 g 10 g

2 L 120 g 60 g 10 g 20 g