1x, 10x TBE (20 liters, in the carboy by the sink)

 

20 L 1x

1 L 10x

1. Tris

      216 g

      108 g

2. Boric Acid

      110 g

      55 g

3. EDTA

      18.6 g

      9.3 g

4. H2O

___ to 20 liter mark, stir overnight at room temp

 

 

 

 

1X TBS (20 liters, in the 4 degree carboy)

 

20 L 1x

 

1. 1M Tris, pH 7.5

      200 ml

    

2. 5M NaCl

       600 ml

    

3. H2O

      to 20 liter mark

    

 

 

 

PBS (8 liters, in the 4 degree carboy)

 

8 L 1x

 

1. NaCl

      64 g

    

2. KCl

       1.6 g

    

3. Na2HPO4

      11.52 g

    

4. KH2PO4

___1.92 g

 

5. H2O

___ to 8 liter mark, stir overnight at room temperature

 

 

 

 

PBS

 

1 L

2 L

8 L

1. DI H2O

      800 ml

      1.6 L

      6.4 L

2. NaCl

      8g

      16 g

      64 g

3. KCl

      0.2 g

      0.4 g

      1.6 g

4. Na2HPO4

      1.44 g

      5.434 g

      11.52 g

5. KH2PO4

      0.24 g

      0.48 g

      1.92 g

pH to 7.4 w/ HCl

 

 

 

Bring up to volume

 

 

 

 

 

 

50X TAE

 

 

1. Trizma base

2. Glacial Acetic Acid

3. 0.5M EDTA (18.16 g EDTA/100 ml)

4. pH to 8.0

5. Autoclave

1L

 

     242 g

     57.1 g

     100 ml

2 L

 

     484 g

     114 ml

     200 ml    

 

20X SSC

 

 

1. Sodium Chloride

2. Sodium Citrate (hydrous)

3. Conc. HCl to pH 7.0

4. Autoclave

1L

 

     175 g

     88.3 g

1.5 L

 

     263 g

     132 g

2 L

 

     350 g

     166 g

 

 

 

 

 

 

 

STERILE PBS

Dissolve in 800 ml dH2O:

            NaCl                8 g

            KCl                 0.2 g

            Na2HPO4        1.44 g

            KH2PO4          0.24 g

 

Adjustt the pH to 7.4 with HCl.

Bring up to 1L with dH2O.

Dispense solution into aliquots and sterilize.

Store at room temperature.