YPD + G418 (300 µg/ml) plates

1. Yeast Extract 2. Bactopeptone 3. Agar 4. Autoclave and cool to 50°C 5. 100x Ade solution 6. 100x Ura solution 7. 50% Glucose 8. G418 stock (80 mg/ml)

1L 10 g 20 g 20 g 10 ml 10 ml 40 ml 5.04 ml

1.5 L 15 g 30 g 30 g 15 ml 15 ml 60 ml 7.56 ml

2 L 20 g 40 g 40 g 20 ml 20 ml 80 ml 10.08 ml

YPD + clonNAT (100 µg/ml) plates

1. Yeast Extract 2. Bactopeptone 3. Agar 4. Autoclave and cool to 50°C 5. 100x Ade solution 6. 100x Ura solution 7. 50% Glucose 8. ClonNAT stock (200 mg/ml)

1 L 10 g 20 g 20 g 10 ml 10 ml 40 ml 0.5 ml

1.5 L 15 g 30 g 30 g 15 ml 15 ml 60 ml 0.75 ml

2 L 20 g 40 g 40 g 20 ml 20 ml 80 ml 1.0 ml