Z-Buffer (Miller 1972)

1. Na2HPO4·7H2O 2. NaH2PO4·H2O 3. KCl 4. MgSO4·7H2O 5. DI H2O 6. pH to 7.0 using HCL 7. bring up to final volume with DI H2O 8. Aliquot 500 ml per 1L bottle 9. Autoclave

1 L 16.1 g 5.5 g 0.75 g 0.24 g ~800 ml

2L 32.2 g 11.0 g 1.5 g 0.492 g ~1600 ml

Western Transfer Buffer (not for the Invitrogen gel system)

	1 L	10 L	20 L
Glycine	2.9 g	29 g	58 g
Tris Base	5.8 g	58 g	116 g Trizma Base
SDS	0.37 g	3.7 g	7.4 g
Methanol	200 ml	2 L	4 L

1x SDS Gel Running Buffer (20 L) (not for the Invitrogen gel system)

	4 L	7.5 L	20 L
Tris Base	12.12 g	22.73 g	60.6 g
Glycine	75.08 g	140.78 g	375.4 g
10% SDS	40 ml	75 ml	200 ml
dH2O	up to 4 L	up to 7.5 L	up to 20 L