Allyl alcohol plates

1 L 1.5 L

1. Yeast Extract 2. Bactopeptone 3. Agar 4. Autoclave and cool to 50°C 5. 50% Glucose 6. 100x Adenine solution 7. 100x Uracil solution 8. For 20mM AA 9. For 2mM AA

10 g 20 g 20 g 40 ml 10 ml 10 ml 1.4 ml 0.14 ml

15 g 30 g 30 g 60 ml 15 ml 15 ml 2.1 ml 0.21 ml