LB Amp or Tet

1. Tryptone 2. Yeast Extract 3. Sodium Chloride 4. 10N NaOH to pH 7.5 5. Agar 6. Autoclave and cool to 50°C AMP Plates: 7. Dissolve ampicillin in Sterile H2O. 8. Filter sterilize and add to media. TET Plates: 9. Dissolve tetracycline in Sterile H2O. 10. Filter sterilize and add to media.

1 L 10 g 5 g 5 g 14 g 70 mg 10 ml 30 mg 10 ml

1.5 L 15 g 7.5 g 7.5 g 21 g 105 mg 15 ml 45 mg 15 ml

2 L 20 g 10 g 10 g 28 g 140 mg 20 ml 60 mg 20 ml