Drop out plates (-his, -leu, -trp, -ura, etc.)

*****Do not use this recipe for Drop-out plates +G418, ClonNAT or hygromycin.****

(See the recipes for “Dropout plates with G418…”)

Drop out plates (aa - )

1. Yeast Nitrogen Base w/o aa and AmSO4 2. ammonium sulfate 3. tyrosine 4. agar 5. autoclave and cool to 50°C 6. 100x Ade solution 7. 100x Ura solution 8. 50% Glucose 9. Appropriate drop-out solution

1 L 1.7 g 5.0 g 30 mg 20 g 10 ml 10 ml 40 ml 10 ml

1.5 L 2.6 g 7.5 g 45 mg 30 g 15 ml 15 ml 60 ml 15 ml

*****Do not use this recipe for Drop-out plates +G418, ClonNAT or hygromycin.****

(See the recipes for “Dropout plates with G418…”)

Ura - plates

1. Yeast Nitrogen Base w/o aa and AmSO4 2. ammonium sulfate 3. tyrosine 4. agar 5. autoclave and cool to 50°C 6. 100x Ade solution 7. 100x Trp solution 8. 50% Glucose 9. 10% CAA

1 L 1.7 g 5.0 g 30 mg 20 g 10 ml 10 ml 40 ml 20 ml

1.5 L 2.6 g 7.5 g 45 mg 30 g 15 ml 15 ml 60 ml 30 ml

*****Do not use this recipe for Drop-out plates +G418, ClonNAT or hygromycin.****

(See the recipes for “Dropout plates with G418…”)

Trp - plates

1. Yeast Nitrogen Base w/o aa and AmSO4 2. ammonium sulfate 3. tyrosine 4. agar 5. autoclave and cool to 50°C 6. 100x Ade solution 7. 100x Ura solution 8. 50% Glucose 9. 10% CAA

1 L 1.7 g 5.0 g 30 mg 20 g 10 ml 10 ml 40 ml 20 ml

1.5 L 2.6 g 7.5 g 45 mg 30 g 15 ml 15 ml 60 ml 30 ml

*****Do not use this recipe for Drop-out plates +G418, ClonNAT or hygromycin.****

(See the recipes for “Dropout plates with G418…”)

Trp - Ura- plates

1. Yeast Nitrogen Base w/o aa and AmSO4 2. ammonium sulfate 3. tyrosine 4. agar 5. autoclave and cool to 50°C 6. 100x Ade solution 8. 50% Glucose 9. 10% CAA

1 L 1.7 g 5.0 g 30 mg 20 g 10 ml 40 ml 20 ml

1.5 L 2.6 g 7.5 g 45 mg 30 g 15 ml 60 ml 30 ml