Drop Out Plates w/G418, clonNAT or hygromycin

Þ Trp- or Ura- + G418, ClonNAT, or hygromycin

1. Yeast Nitrogen Base w/o aa and AmSO4 2. Glutamic acid (monosodium salt)* 3. tyrosine 4. agar 5. Autoclave and cool to 50°C 6. 100x Ade solution 7. 50% Glucose 8. 10% CAA 9a. 100x Ura solution for Trp- plates OR 9b. 100x Trp solution for Ura- plates (Leave out step 9 for Trp- Ura- plates) 10a. G418 (80 mg/ml) OR 10b. ClonNAT (200 mg/ml) OR 10c. hygromycin (check container for concentration, final is 300 microg/ml)

1 L 1.7 g 1 g 30 mg 20 g 10 ml 40 ml 20 ml 10 ml 10 ml 5.04 ml 500 microliters

Drop-Out Plates with G418, ClonNAT, or hygromycin

Þ any type except Trp- or Ura- (e.g. Leu- or Trp- or His-) + G418, ClonNAT, or hygromycin

1. Yeast Nitrogen Base w/o aa and AmSO4 2. Glutamic acid (monosodium salt)* 3. tyrosine 4. agar 5. Autoclave and cool to 50°C 6. 100x Trp solution 7. 100x Ade solution 8. 100x Ura solution 9. 50% Glucose 10. drop-out solution (Leu-, Trp-, His-, etc.) 11a. G418 (80 mg/ml) OR 11b. ClonNAT (200 mg/ml) OR 11c. hygromycin (check container for concentration, final is 300 microg/ml)

1 L 1.7 g 1 g 30 mg 20 g 10 ml 10 ml 10 ml 40 ml 10 ml 5.04 ml 500 microliters