Stock solutions for the shelves

Solution 1 M Tris Desired pH: pH 7.4 pH 7.6 pH 8.0	Method of Preparation Dissolve 121.1 g tris base in 800 ml of H2O. Adjust the pHt to the desire value by adding concentrated HCl. Approximate amount of concentrated HCl: 70 ml 60 ml 42 ml Allow the solution to cool to room temperature before making final adjustments to the pH. Make up the volume of the solution to 1 liter. Dispense into aliquots and sterilize by autoclaving.	If the 1 M solution has a yellow color, discard it and obtain a better quality Tris. Although many types of electrodes do not accurately measure the pH of Tris solutions, suitable electrodes and be obtained from most manufacturers.
50% glucose	Slowly add 500g of glucose (dextrose) to ~500 ml dH2O. Microwave 5 minutes. Bring level up to 1000 ml with dH2O. Aliquot 100 ml per bottle. Autoclave.
0.5 M EDTA (pH 8.0)	Add 186.1 g of disodium ethylene tetraacetate·2H2O to 800 ml of H2O. Stir vigorously on a magnetic stirrer. Adjust pH to 8.0 with NaOH (~20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving.	The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to ~8.0 by the addition of NaOH.
5 M NaCl	Dissolve 292.2 g of NaCl in 800 ml of H2O. Adjust volume to 1 liter. Dispense into aliquots and sterilize by autoclaving.
1 M MgCl2	Dissolve 203.3 g of MgCl2·6H2O in 800 ml of H2O. Adjust volume to 1 liter. Dispense into aliquots and sterilize by autoclaving.	MgCl2 is extremely hygroscopic. Buy in small bottles (e.g. 100 g) and do not store opened bottles for long periods of time.
3 M Sodium acetate (pH 5.2)	Dissolve 408.1 g sodium acetate·3H2O in 800 ml of H2O. Adjust pH to 5.2 with glacial acetic acid. Adjust volume to 1 liter. Dispense into aliquots and sterilize by autoclaving.
1 M Dithiothreitol (DTT)	Dissolve 3.09 g of DTT in 20 ml of 0.01 M sodium acetate (pH 5.2). Filter sterilize. Dispense into 1 ml aliquots and store at -20°C.	Do not autoclave DTT or solutions containing DTT.
B-Mercaptoethanol (BME)	Usually obtained as a 14.4 M solution. Store in a dark bottle at 4°C.	Do not autoclave BME or solutions containing BME.
10% Sodium dodecyl sulfate (SDS) [aka sodium lauryl sulfate]	Dissolve 100 g of electrophoresis-grade SDS in 900 ml of H2O. Heat to 68°C to assist dissolution. Adjust pH to 7.2 by adding a few drops of conc. HCl. Adjust volume to 1 liter. Dispense into aliquots.	Wear a mask when weighing SDS. There is no need to sterilize 10% SDS
1 M Magnesium acetate	Dissolve 214.46 g of magnesium acetate· 4H2O in 800 ml of H2O. Adjust volume to 1 liter. Filter sterilize.
5 M Ammonium acetate	Dissolve 385 g of ammonium acetate in 800 ml of H2O. Adjust volume to 1 liter. Filter sterilize.
5 M Potassium Acetate (3 M KOAc)	To 60 ml of 5 M potassium acetate add 11.5 ml of glacial acetic acid and 28.5 ml of H2O. The resulting solution is 3 M with respect to potassium and 5 M with respect to acetate
20x SSX	Dissolve 175.3 g of NaCl and 88.2 g of sodium citrate·2H2O in 800 ml of H2O. Adjust pH to 7.0 with a few drops of a 10 N solution of NaOH. Adjust volume to 1 liter. Dispense into aliquots and filter sterilize.
20x SSPE	Dissolve 174 g of NaCl, 27.6 g of NaH2PO4·H2O and 7.4 g of EDTA in 800 ml of H2O. Adjust pH to 7.4 with NaOH (~6.5 ml of a 10 N solution). Adjust the volume to 1 liter. Dispense into aliquots. Filter sterilize.
Ethidium bromide, 10 mg/ml	Add 1 g of ethidium bromide to 100 ml of H2O. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolve. Wrap the container in foil or transfer to a dark bottle and store at 4°C.	Ethidium bromide is a powerful mutagen. Wear gloves, a lab coat and a mask when weighing it out.
Trichloracetic acid (TCA), 100% solution	To a bottle containing 500 g of TCA, add 227 ml of H2O. The resulting solution will contain 100% (w/v) TCA.	Handle carefully. Wear gloves and a lab coat. Work with TCA in the hood.
NaD aliquots	Prepare a 25 nM solution to place in 6 ml sterile tubes in 1 ml, 2 ml and 3 ml quantities. MW NaD = 663.5 To make 200 ml of solution: [663.5 g/L] * [0.2 L] = 132 g * 0.025 = 3.3 g Place 3.3 g NaD in 200 ml sterile dH2O and distribute it after it dissolves. Freeze and place in marked boxes in the freezer. 0.825 g/ 50 mls