Our research focuses on the regulation of myogenic stem cell function in adult life. Our long-term goal is to identify means to ameliorate age-related muscle deterioration (sarcopenia) and combat muscle wasting in muscular dystrophy. We investigate satellite cells, classically defined tissue specific myogenic stem cells that reside beneath the myofiber basal lamina, as well as non-myogenic progenitors associated with the microvasculature that may contribute to myogenesis by myogenic reprogramming.
The following research areas are pursued:
- Mechanisms involved in supporting myogenic commitment and renewal of satellite cells.
The role of FGF-FGFR system in regulating satellite cells.
The role of Klotho genes in the balance between myogenicity and adiposity of skeletal muscle.
- Origin and cellular/molecular distinctions of satellite cells from extraocular muscles (EOM) that contribute to enhanced stem cell performance and sparing from muscular dystrophy.
- Origin and significance of unconventional progenitors that may function to replace myonuclei during myofiber maintenance. Emphasis is given to the role of cells associated with the microvasculature, in particular the pericytes.
Current funding: National Institutes of Health
Publications [from PubMed]
- Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell.Stuelsatz P, Keire P, Yablonka-Reuveni ZMethods in molecular biology (Clifton, N.J.). 2017 ; 1556 : 51-102
- Expression profile and overexpression outcome indicate a role for βKlotho in skeletal muscle fibro/adipogenesis.Phelps M, Stuelsatz P, Yablonka-Reuveni ZThe FEBS journal. 2016 May; 283 9: 1653-68
- Myogenic-specific ablation of Fgfr1 impairs FGF2-mediated proliferation of satellite cells at the myofiber niche but does not abolish the capacity for muscle regeneration.Yablonka-Reuveni Z, Danoviz ME, Phelps M, Stuelsatz PFrontiers in aging neuroscience. 2015 ; 7 : 85
- Extraocular muscle satellite cells are high performance myo-engines retaining efficient regenerative capacity in dystrophin deficiency.Stuelsatz P, Shearer A, Li Y, Muir LA, Ieronimakis N, Shen QW, Kirillova I, Yablonka-Reuveni ZDevelopmental biology. 2015 Jan; 397 1: 31-44
- Phelps M, Pettan-Brewer C, Ladiges W, Yablonka-Reuveni ZBiogerontology. 2013 Dec; 14 6: 729-39