PEOPLE
FACULTY
FACULTY
STAFF
STAFF
GRADUATE STUDENTS
GRADUATE STUDENTS
POSTDOCTORAL FELLOWS
POSTDOCTORAL FELLOWS
Liangcai Gu
Liangcai Gu
Assistant Professor of Biochemistry and the Institute for Protein Design
Adjunct Assistant Professor of Genome Sciences
B.S. 1998, Lanzhou University
M.S. 2002, Tsinghua University
Ph.D. 2008, University of Michigan
Off.: J583
 
Ph.: 206.221.7730
 
Fax: 206.897.1335

Research

We use quantitative protein interaction profiling to understand molecular recognition and guide computational protein design. We develop protein interaction sequencing technologies by coupling ‘protein barcoding’ techniques—e.g., molecular attachment of proteins to barcoding DNAs, display of proteins on phage or cell surfaces, and indirect barcoding of unlabeled proteins by using DNA barcoded affinity reagents such as antibodies—to massively parallel 'in situ DNA sequencing' to quantitate protein interactions at a single-molecule or single-cell level. One example of protein interaction sequencing is a single-molecular-interaction sequencing (SMI-seq) technology (Gu, et al., Nature, 2014) for large-scale molecular counting of proteins and complexes. Our current research interests include (1) engineering of ligand-responsive protein assemblies, (2) human protein interactome profiling and drug screening, and (3) functional profiling of B-cell and T-cell receptors.

Publications:

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