NK Cell Functional Assays

  • NK cell immunophenotyping: can include inhibitory and activating receptors for NK cells.
  • Viral Suppression Assay (VSA): Measures the ability of NK cells to inhibit viral replication in autologous CD4 cells. This inhibition measures a combination of both direct cytotoxicity and chemokine/cytokine secretion.
  • Cytotoxicity Assay: Measures the direct killing activity of NK cells when they encounter HIV-infected targets. This is a flow-based assay.
  • Antibody-dependent Cell-mediated Viral Inhibition (ADCVI): Measures the ability of HIV-specific antibodies to mediate killing of HIV-infected cells via NK cell activation. Assay can be performed with autologous or heterologous NK cells.

For more information on how to access these services, contact Ana Gervassi at ana.gervassi at seattlebiomed.org.