HHV8 Real Time Quantitative PCR on all types of sample: Plasma, Serum, Saliva, Swabs, Whole Blood, etc.
Viruses may be present in a variety of clinical samples including saliva, genital secretion, CSF, plasma, serum, body fluids and tissues. The viral DNA can be extracted and quantified using PCR techniques. The extraction of DNA from the sample can be done by different procedures with different chemistry. Qiagen chemistry and single column procedure, which have been proved to be effective and efficient, have been adopted and is currently used by our lab.
After DNA is extracted, Real-Time Taqman PCR technology utilizing fluorescent labeled probes is used to amplify, detect and quantify a variety of HHV8 viral DNA in the sample.
Primers and probes specific to the ORF73 and T.07-K12 regions of the HHV8 genome have been designed and tested for sensitivity and specificity at the University of Washington Molecular Diagnostic Lab in Seattle, Washington, USA. An internal control is spiked into each PCR reaction to ensure that no non-specific inhibition of the reaction has taken place. A negative result is accepted only if the internal control amplification is detected.